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Yeasen Biotechnology superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+reverse+transcriptase/reverse+transcriptase/pmc13191876-60-21-34
Average 86 stars, based on 1 article reviews
superscript iii reverse transcriptase - by Bioz Stars, 2026-09
86/100 stars

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Reverse Transcription:

Article Title: CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts
Article Snippet: Total RNA was efficiently extracted using TRIzol reagent (15596026CN; Invitrogen, Waltham, MA, USA). .. Subsequently, Prime Script reverse transcriptase (11141ES60; Yeasen, Shanghai, China) was used for reverse transcribed the RNA into cDNA. qPCR SYBR Green Master Mix (11184ES08; Yeasen, Shanghai, China) was applied following the manufacturer’s instructions precisely. ..

Article Title: Growth hormone-releasing peptide 6 (GHRP-6) hydrogel for acute kidney injury therapy via metabolic regulation
Article Snippet: Total RNA was extracted from cells or tissues via TRIzol reagent (Takara, Japan) following the manufacturer’s instructions. .. Then, Reverse Transcriptase SuperMix (YEASEN, China) was used to reverse transcribe the RNA to synthesize complementary DNA (cDNA). .. Quantitative RT‒PCR analysis was performed via qPCR SYBR (YEASEN, China) on a CFX96 Touch System (Bio-Rad, CA).

Article Title: CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts.
Article Snippet: Total RNA was efficiently extracted using TRIzol reagent (15596026CN; Invitrogen, Waltham, MA, USA). .. Subsequently, Prime Script reverse transcriptase (11141ES60; Yeasen, Shanghai, China) was used for reverse transcribed the RNA into cDNA. qPCR SYBR Green Master (See figure on previous page.) ..

Article Title: Astragalus licorice prescription and its active components alleviate chemotherapy-induced intestinal mucositis by apoptosis and fatty acid β-oxidation: Integrative multi-omics approaches.
Article Snippet: Background: Chemotherapy-induced intestinal mucositis (CIM) is one of the most common side effects of chemotherapy agents.. Astragalus licorice prescription (ALP), a traditional Chinese formula, commonly used to treat gastrointestinal disorders, has an unclear mechanism and potential active components in alleviating CIM.. Purpose: This study aims to comprehensively explore the mechanism and bioactive components of ALP in alle-

Article Title: Expansion of the plant-parasitic nematode RNA viruses: Unprecedented diversity, intron-bearing viruses, and cross-kingdom evolutionary links
Article Snippet: For cloning of full-length viral sequences, a PC3-T7 loop adapter was ligated to the RNA termini using T4 RNA ligase (TaKaRa) at 16 °C. .. The ligated products were reverse-transcribed into cDNA using M-MLV reverse transcriptase, and the terminal regions were amplified using Taq DNA polymerase (Yeasen Biotechnology Co., Ltd.) with the PC2 primer and virus-specific primers. ..

Article Title: Prenatal phenotypic delineation of a de novo EYA1 likely pathogenic variant in branchio-oto-renal syndrome
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1 st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified. ..

Real-time Polymerase Chain Reaction:

Article Title: CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts
Article Snippet: Total RNA was efficiently extracted using TRIzol reagent (15596026CN; Invitrogen, Waltham, MA, USA). .. Subsequently, Prime Script reverse transcriptase (11141ES60; Yeasen, Shanghai, China) was used for reverse transcribed the RNA into cDNA. qPCR SYBR Green Master Mix (11184ES08; Yeasen, Shanghai, China) was applied following the manufacturer’s instructions precisely. ..

Article Title: CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts.
Article Snippet: Total RNA was efficiently extracted using TRIzol reagent (15596026CN; Invitrogen, Waltham, MA, USA). .. Subsequently, Prime Script reverse transcriptase (11141ES60; Yeasen, Shanghai, China) was used for reverse transcribed the RNA into cDNA. qPCR SYBR Green Master (See figure on previous page.) ..

Article Title: Prenatal phenotypic delineation of a de novo EYA1 likely pathogenic variant in branchio-oto-renal syndrome
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1 st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified. ..

SYBR Green Assay:

Article Title: CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts
Article Snippet: Total RNA was efficiently extracted using TRIzol reagent (15596026CN; Invitrogen, Waltham, MA, USA). .. Subsequently, Prime Script reverse transcriptase (11141ES60; Yeasen, Shanghai, China) was used for reverse transcribed the RNA into cDNA. qPCR SYBR Green Master Mix (11184ES08; Yeasen, Shanghai, China) was applied following the manufacturer’s instructions precisely. ..

Article Title: CAMKK2 restored mitochondrial dynamics homeostasis to alleviate pulmonary fibrosis via AMPK/PGC-1α signaling pathway in lung fibroblasts.
Article Snippet: Total RNA was efficiently extracted using TRIzol reagent (15596026CN; Invitrogen, Waltham, MA, USA). .. Subsequently, Prime Script reverse transcriptase (11141ES60; Yeasen, Shanghai, China) was used for reverse transcribed the RNA into cDNA. qPCR SYBR Green Master (See figure on previous page.) ..

other:

Article Title: PRMT5/Sohlh2/Sirt1 Signaling Pathway in Vascular Endothelial Cells Modulates Lung Metastasis of Triple-Negative Breast Cancer.
Article Snippet: TRIZOL reagent (Invitrogen) was used to extract RNA according to the manufacturer’s instructions and was reverse transcribed using reverse transcriptase (YEASEN).

Isolation:

Article Title: Astragalus licorice prescription and its active components alleviate chemotherapy-induced intestinal mucositis by apoptosis and fatty acid β-oxidation: Integrative multi-omics approaches.
Article Snippet: Background: Chemotherapy-induced intestinal mucositis (CIM) is one of the most common side effects of chemotherapy agents.. Astragalus licorice prescription (ALP), a traditional Chinese formula, commonly used to treat gastrointestinal disorders, has an unclear mechanism and potential active components in alleviating CIM.. Purpose: This study aims to comprehensively explore the mechanism and bioactive components of ALP in alle-

Amplification:

Article Title: Expansion of the plant-parasitic nematode RNA viruses: Unprecedented diversity, intron-bearing viruses, and cross-kingdom evolutionary links
Article Snippet: For cloning of full-length viral sequences, a PC3-T7 loop adapter was ligated to the RNA termini using T4 RNA ligase (TaKaRa) at 16 °C. .. The ligated products were reverse-transcribed into cDNA using M-MLV reverse transcriptase, and the terminal regions were amplified using Taq DNA polymerase (Yeasen Biotechnology Co., Ltd.) with the PC2 primer and virus-specific primers. ..

Virus:

Article Title: Expansion of the plant-parasitic nematode RNA viruses: Unprecedented diversity, intron-bearing viruses, and cross-kingdom evolutionary links
Article Snippet: For cloning of full-length viral sequences, a PC3-T7 loop adapter was ligated to the RNA termini using T4 RNA ligase (TaKaRa) at 16 °C. .. The ligated products were reverse-transcribed into cDNA using M-MLV reverse transcriptase, and the terminal regions were amplified using Taq DNA polymerase (Yeasen Biotechnology Co., Ltd.) with the PC2 primer and virus-specific primers. ..

Cell Culture:

Article Title: Prenatal phenotypic delineation of a de novo EYA1 likely pathogenic variant in branchio-oto-renal syndrome
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1 st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified. ..

cDNA Synthesis:

Article Title: Prenatal phenotypic delineation of a de novo EYA1 likely pathogenic variant in branchio-oto-renal syndrome
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1 st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified. ..

Polymerase Chain Reaction:

Article Title: Prenatal phenotypic delineation of a de novo EYA1 likely pathogenic variant in branchio-oto-renal syndrome
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1 st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified. ..



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