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Promega wizard lambda preps kit
Wizard Lambda Preps Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+lambda+system+kit/wizard+lambda+prep+kit/pmc00111920-91-7-12
Average 90 stars, based on 1 article reviews
wizard lambda preps kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Clone Assay:

Article Title: Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene
Article Snippet: .. Small-scale phage DNAs were extracted from the rGSTA3positive clones using a Wizard lambda preps kit (Promega). ..

Article Title: Cloning and Sequence Analysis of a Highly Polymorphic Cryptosporidium parvum Gene Encoding a 60-Kilodalton Glycoprotein and Characterization of Its 15- and 45-Kilodalton Zoite Surface Antigen Products
Article Snippet: The libraries were screened in duplicate using a mixture of hybridoma culture supernatants containing monoclonal IgA antibodies CrA1 and CrA2; positive clones were detected using alkaline phosphatase-conjugated goat anti-mouse IgA secondary antibody (Southern Biotechnology Associates) followed by colorimetric development with nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP). .. Bacteriophage DNA from positive λgt11 clones was prepared using a Lambda Wizard Prep kit (Promega), digested with Eco RI, and fractionated by agarose gel electrophoresis. .. The recombinant DNA inserts were excised from the gel, purified using QIAEX II resin (Qiagen), ligated into Eco RI-cut phosphatase-treated pBlueScript II SK− plasmid (Stratagene), and transformed into E. coli JM109.

Article Title: The SSX gene family: characterization of 9 complete genes.
Article Snippet: Human SSX genes comprise a gene family with 6 known members.. SSX1, 2 and 4 have been found to be involved in the t(X;18) translocation characteristically found in all synovial sarcomas.. Four (SSX1, 2, 4 and 5) are known to be expressed in a subset of tumors and testis, and anti-SSX antibodies have been found in sera from cancer patients.

Article Title: Cloning and Sequence Analysis of a Highly Polymorphic Cryptosporidium parvum Gene Encoding a 60-Kilodalton Glycoprotein and Characterization of Its 15- and 45-Kilodalton Zoite Surface Antigen Products
Article Snippet: The libraries were screened in duplicate using a mixture of hybridoma culture supernatants containing monoclonal IgA antibodies CrA1 and CrA2; positive clones were detected using alkaline phosphatase-conjugated goat anti-mouse IgA secondary antibody (Southern Biotechnology Associates) followed by colorimetric development with nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP). .. Bacteriophage DNA from positive lgt11 clones was prepared using a Lambda Wizard Prep kit (Promega), digested with EcoRI, and fractionated by agarose gel electrophoresis. .. The recombinant DNA inserts were excised from the gel, purified using QIAEX II resin (Qiagen), ligated into EcoRI-cut phosphatase-treated pBlueScript II SK2 plasmid (Stratagene), and transformed into E. coli JM109.

Article Title: Human kcnq5 potassium channel, methods and compositions thereof
Article Snippet: .. DNA was isolated from plate lysates of the positive clones and purified using Promega's Wizard Lambda Prep kit. .. The DNA was enzymatically digested with EcoRI, and inserts were gel-purified using Clontech's Nucleotrap Kit.

Isolation:

Article Title: Methods of preparing and using a viral vector library
Article Snippet: .. Approximately 20 μg of phage DNA was isolated from 50,000 plaques utilizing a Promega Wizard Lambda Prep kit. .. The phage DNA was digested with SwaI and SpeI and purified using Qiagen's plasmid purification cartridge.

Article Title: Human kcnq5 potassium channel, methods and compositions thereof
Article Snippet: .. DNA was isolated from plate lysates of the positive clones and purified using Promega's Wizard Lambda Prep kit. .. The DNA was enzymatically digested with EcoRI, and inserts were gel-purified using Clontech's Nucleotrap Kit.

Article Title: Nucleic acids encoding interphotoreceptor matrix proteins
Article Snippet: .. Inserts contained within λgt11 are isolated using the Wizard Lambda Prep kit (Promega), released by EcoR1 digestion and subsequently ligated into pBluescript. .. Plasmid DNA is isolated using Wizard miniprep resins (Promega) and assayed for insert size by restriction enzyme digest and size fractionation on 0.7-1.5% agarose gels. i. Anchored PCR. cDNA amplification using one-sided, or “anchored,” PCR can be used to obtain missing sequence information.

Agarose Gel Electrophoresis:

Article Title: Cloning and Sequence Analysis of a Highly Polymorphic Cryptosporidium parvum Gene Encoding a 60-Kilodalton Glycoprotein and Characterization of Its 15- and 45-Kilodalton Zoite Surface Antigen Products
Article Snippet: The libraries were screened in duplicate using a mixture of hybridoma culture supernatants containing monoclonal IgA antibodies CrA1 and CrA2; positive clones were detected using alkaline phosphatase-conjugated goat anti-mouse IgA secondary antibody (Southern Biotechnology Associates) followed by colorimetric development with nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP). .. Bacteriophage DNA from positive λgt11 clones was prepared using a Lambda Wizard Prep kit (Promega), digested with Eco RI, and fractionated by agarose gel electrophoresis. .. The recombinant DNA inserts were excised from the gel, purified using QIAEX II resin (Qiagen), ligated into Eco RI-cut phosphatase-treated pBlueScript II SK− plasmid (Stratagene), and transformed into E. coli JM109.

Article Title: Cloning and Sequence Analysis of a Highly Polymorphic Cryptosporidium parvum Gene Encoding a 60-Kilodalton Glycoprotein and Characterization of Its 15- and 45-Kilodalton Zoite Surface Antigen Products
Article Snippet: The libraries were screened in duplicate using a mixture of hybridoma culture supernatants containing monoclonal IgA antibodies CrA1 and CrA2; positive clones were detected using alkaline phosphatase-conjugated goat anti-mouse IgA secondary antibody (Southern Biotechnology Associates) followed by colorimetric development with nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP). .. Bacteriophage DNA from positive lgt11 clones was prepared using a Lambda Wizard Prep kit (Promega), digested with EcoRI, and fractionated by agarose gel electrophoresis. .. The recombinant DNA inserts were excised from the gel, purified using QIAEX II resin (Qiagen), ligated into EcoRI-cut phosphatase-treated pBlueScript II SK2 plasmid (Stratagene), and transformed into E. coli JM109.

Recombinant:

Article Title: Isolation and Characterization of an Equine Foamy Virus
Article Snippet: .. Lambda recombinant DNA was purified using a Wizard Lambda Preps kit from Promega. ..

Purification:

Article Title: Isolation and Characterization of an Equine Foamy Virus
Article Snippet: .. Lambda recombinant DNA was purified using a Wizard Lambda Preps kit from Promega. ..

Article Title: The SSX gene family: characterization of 9 complete genes.
Article Snippet: Human SSX genes comprise a gene family with 6 known members.. SSX1, 2 and 4 have been found to be involved in the t(X;18) translocation characteristically found in all synovial sarcomas.. Four (SSX1, 2, 4 and 5) are known to be expressed in a subset of tumors and testis, and anti-SSX antibodies have been found in sera from cancer patients.

Article Title: Human kcnq5 potassium channel, methods and compositions thereof
Article Snippet: .. DNA was isolated from plate lysates of the positive clones and purified using Promega's Wizard Lambda Prep kit. .. The DNA was enzymatically digested with EcoRI, and inserts were gel-purified using Clontech's Nucleotrap Kit.

Library Screening:

Article Title: The SSX gene family: characterization of 9 complete genes.
Article Snippet: Human SSX genes comprise a gene family with 6 known members.. SSX1, 2 and 4 have been found to be involved in the t(X;18) translocation characteristically found in all synovial sarcomas.. Four (SSX1, 2, 4 and 5) are known to be expressed in a subset of tumors and testis, and anti-SSX antibodies have been found in sera from cancer patients.

Amplification:

Article Title: The SSX gene family: characterization of 9 complete genes.
Article Snippet: Human SSX genes comprise a gene family with 6 known members.. SSX1, 2 and 4 have been found to be involved in the t(X;18) translocation characteristically found in all synovial sarcomas.. Four (SSX1, 2, 4 and 5) are known to be expressed in a subset of tumors and testis, and anti-SSX antibodies have been found in sera from cancer patients.

Reverse Transcription Polymerase Chain Reaction:

Article Title: The SSX gene family: characterization of 9 complete genes.
Article Snippet: Human SSX genes comprise a gene family with 6 known members.. SSX1, 2 and 4 have been found to be involved in the t(X;18) translocation characteristically found in all synovial sarcomas.. Four (SSX1, 2, 4 and 5) are known to be expressed in a subset of tumors and testis, and anti-SSX antibodies have been found in sera from cancer patients.



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