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superscript lambda system cdna synthesis k cloning kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript lambda system cdna synthesis k cloning kit
    Superscript Lambda System Cdna Synthesis K Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript%E2%84%A2+lambda+system+kit/pm15519295-35-56-62
    Average 90 stars, based on 1 article reviews
    superscript lambda system cdna synthesis k cloning kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Glycemic Variability Promotes Both Local Invasion and Metastatic Colonization by Pancreatic Ductal Adenocarcinoma
    Article Snippet: RNA extraction (74104; Qiagen) and cDNA synthesis (K1632; Thermo Fisher Scientific) were conducted according to the manufacturer’s instructions.

    Article Title: Whole genome transcriptome polymorphisms in Arabidopsis thaliana
    Article Snippet: Poly-(A) RNA was mixed with 166 ng random hexamer (Invitrogen) and subjected to first-strand cDNA synthesis (Invitrogen) according to the manufacturer's recommendations in a total volume of 40 μl at 42°C for 1 h. The 40 μl first-strand reaction was used in second-strand cDNA synthesis (Invitrogen) according to the manufacturer's recommendations in a total volume of 300 μl at 16°C for 2 h. Samples were then subjected to RNase treatment at 37°C for 20 minutes with 20 units RNaseH (Epicentre, Madison, Wisconsin, USA), 1 unit RNaseA and 40 units RNaseT (Ambion, Austin, Texas, USA).

    Article Title: Activated B-cells and Plasma cells are Resistant to Radiation Therapy
    Article Snippet: RNA (100 ng) was used for cDNA synthesis (Superscript IV VILO, Thermo Fisher Scientific, CA).

    Article Title: Characterization of a germline variant MSH6 c. 4001G > C in a Lynch syndrome family
    Article Snippet: Total RNA from the patient was extracted using the PAXgene BloodRNA Kit (PreAnalytiX, Qiagen, Valencia, CA) and was subsequently used for cDNA synthesis (Superscript III First‐Strand Synthesis SuperMix, Invitrogen Life Technologies, Carlsbad, CA).

    Article Title: Dual effects of noradrenaline on astroglial production of chemokines and pro-inflammatory mediators
    Article Snippet: TRIzol, Taq polymerase and cDNA synthesis reagents were obtained from Invitrogen (Carlsbad, CA, USA).

    Generated:

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)
    Article Snippet: .. Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD). ..

    cDNA Synthesis:

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)
    Article Snippet: .. Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD). ..

    Article Title: A better brain? Alternative spliced STIM2 in hominoids arises with synapse formation and creates a gain-of-function variant
    Article Snippet: .. For cDNA synthesis, SuperScriptTM II Reverse Transcriptase (Life Technologies) was used. qRT-PCRs were performed using QuantiTect SYBR Green Kit (Qiagen) and a CFX96 Real-Time System (Bio-Rad). ..

    Article Title: MicroRNA-221/222-expression in HSC and MPP safeguards their quiescence and multipotency by downregulating stress-independent and dependent expression of IEG and of several myelo/granulopoiesis-enhancing target genes
    Article Snippet: Isolated RNA was then quantified and qualified by Fragment Analyzer with the HS NGS Fragment Kit (1–6000 bp) (Agilent). .. RT-PCR was performed using SuperScript IV Reverse Transcriptase (Invitrogen) cDNA synthesis reaction and Oligo d(T)18 primer (Thermo Scientific) according to the user guide. .. Quantitive PCR was performed using QuantiTect SYBR Green PCR Master Mix (Qiagen) according to the user guide using following primer sets: Fos F: 5’-GCCCAGTGAGGAATATCTGGA-3’, R: 5’-ATCGCAGATGAAGCTCTGGT-3’, and Hprt F: 5’- AAGCTTGCTGGTGAAAAGGA-3’, R: 5’- TTGCGCTCATCTTAGGCTTT-3’ as endogenous control.

    Reverse Transcription:

    Article Title: A better brain? Alternative spliced STIM2 in hominoids arises with synapse formation and creates a gain-of-function variant
    Article Snippet: .. For cDNA synthesis, SuperScriptTM II Reverse Transcriptase (Life Technologies) was used. qRT-PCRs were performed using QuantiTect SYBR Green Kit (Qiagen) and a CFX96 Real-Time System (Bio-Rad). ..

    Article Title: MicroRNA-221/222-expression in HSC and MPP safeguards their quiescence and multipotency by downregulating stress-independent and dependent expression of IEG and of several myelo/granulopoiesis-enhancing target genes
    Article Snippet: Isolated RNA was then quantified and qualified by Fragment Analyzer with the HS NGS Fragment Kit (1–6000 bp) (Agilent). .. RT-PCR was performed using SuperScript IV Reverse Transcriptase (Invitrogen) cDNA synthesis reaction and Oligo d(T)18 primer (Thermo Scientific) according to the user guide. .. Quantitive PCR was performed using QuantiTect SYBR Green PCR Master Mix (Qiagen) according to the user guide using following primer sets: Fos F: 5’-GCCCAGTGAGGAATATCTGGA-3’, R: 5’-ATCGCAGATGAAGCTCTGGT-3’, and Hprt F: 5’- AAGCTTGCTGGTGAAAAGGA-3’, R: 5’- TTGCGCTCATCTTAGGCTTT-3’ as endogenous control.

    SYBR Green Assay:

    Article Title: A better brain? Alternative spliced STIM2 in hominoids arises with synapse formation and creates a gain-of-function variant
    Article Snippet: .. For cDNA synthesis, SuperScriptTM II Reverse Transcriptase (Life Technologies) was used. qRT-PCRs were performed using QuantiTect SYBR Green Kit (Qiagen) and a CFX96 Real-Time System (Bio-Rad). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: MicroRNA-221/222-expression in HSC and MPP safeguards their quiescence and multipotency by downregulating stress-independent and dependent expression of IEG and of several myelo/granulopoiesis-enhancing target genes
    Article Snippet: Isolated RNA was then quantified and qualified by Fragment Analyzer with the HS NGS Fragment Kit (1–6000 bp) (Agilent). .. RT-PCR was performed using SuperScript IV Reverse Transcriptase (Invitrogen) cDNA synthesis reaction and Oligo d(T)18 primer (Thermo Scientific) according to the user guide. .. Quantitive PCR was performed using QuantiTect SYBR Green PCR Master Mix (Qiagen) according to the user guide using following primer sets: Fos F: 5’-GCCCAGTGAGGAATATCTGGA-3’, R: 5’-ATCGCAGATGAAGCTCTGGT-3’, and Hprt F: 5’- AAGCTTGCTGGTGAAAAGGA-3’, R: 5’- TTGCGCTCATCTTAGGCTTT-3’ as endogenous control.



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