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super script iii reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher super script iii reverse transcriptase kit
    Super Script Iii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/super+script+iii+reverse+transcriptase+system+kit/pmc11346181-331-10-16
    Average 86 stars, based on 1 article reviews
    super script iii reverse transcriptase kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: A multiplex inhalation platform to model in situ like aerosol delivery in a breathing lung-on-chip
    Article Snippet: Purity and concentration of RNA was analyzed using a Nano-Drop Spectrophotometer (Thermo Fischer Scientific, Switzerland). .. Next, cDNA was transcribed using the Super Script III Reverse Transcriptase kit (Life Technologies, Switzerland). .. Finally, qRT-PCR reactions were performed in triplicates with SYBR ® Select Master Mix (Thermo Scientific) in an ABI7500 Fast (Applied Biosystems) real-time PCR system.

    Article Title: A maternally inherited Chromosomal Passenger Complex regulates germ plasm ribonucleoparticle aggregation in Zebrafish
    Article Snippet: .. Total RNA was extracted from pools of five wild-type embryos using Trizol (Invitrogen) according to manufacturer instructions. cDNA was synthesized using the Super Script III reverse transcriptase kit (Invitrogen) with a random hexamer primer. ..

    Article Title: miR-451a suppresses papillary thyroid cancer cell proliferation and invasion and facilitates apoptosis through targeting DCBLD2 and AKT1.
    Article Snippet: Papillary thyroid cancer (PTC) is a common malignancy.. MicroRNAs (miRNAs) may act as oncogenes or tumor suppressor genes.. However, the role of miR-451a in PTC is not fully understood.

    Article Title: Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients
    Article Snippet: Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc., USA), and RNA was quantified using a Nanodrop 200° ND-1000 UV–vis spectrophotometer (Nanodrop Technologies, USA). .. The reverse transcriptase reaction was performed using 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (Thermo Fisher Scientific, France) in a Thermal Cycler 2720 thermocycler (Applied Biosystems, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). .. PCR amplification was performed using an Absolute Blue SYBR Green Fluorescein Kit (Roche Molecular Biochemicals, France) and run on a StepOnePlus system (Life Technologies).

    Article Title: Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells
    Article Snippet: The RNA amount and purity of each sample were quantified using NanoDrop (ND-1000, Wilmington, DE, USA). .. The mRNA was reverse transcribed into first-strand cDNA using the Super Script III reverse transcriptase kit (Invitrogen, 18,080–085), followed by second-strand cDNA synthesis using the Second Strand cDNA synthesis kit (Bio Wavelet, BWR001). ..

    Article Title: Mechanical Resistance to Micro-Heart Tissue Contractility unveils early Structural and Functional Pathology in iPSC Models of Hypertrophic Cardiomyopathy
    Article Snippet: Cell or tissue lysis and RNA recovery were performed using RNAqueous®-Micro Kit (Thermo Scientific). .. At least 320 ng RNA per sample was synthesized for cDNA using Super Script III Reverse Transcriptase Kit (Thermo Scientific). .. Gene expression was performed using Applied Biosystems Step One Plus Light Cycler instrument using SYBR green master mix (Thermo Scientific).

    Synthesized:

    Article Title: A maternally inherited Chromosomal Passenger Complex regulates germ plasm ribonucleoparticle aggregation in Zebrafish
    Article Snippet: .. Total RNA was extracted from pools of five wild-type embryos using Trizol (Invitrogen) according to manufacturer instructions. cDNA was synthesized using the Super Script III reverse transcriptase kit (Invitrogen) with a random hexamer primer. ..

    Article Title: miR-451a suppresses papillary thyroid cancer cell proliferation and invasion and facilitates apoptosis through targeting DCBLD2 and AKT1.
    Article Snippet: Papillary thyroid cancer (PTC) is a common malignancy.. MicroRNAs (miRNAs) may act as oncogenes or tumor suppressor genes.. However, the role of miR-451a in PTC is not fully understood.

    Article Title: Mechanical Resistance to Micro-Heart Tissue Contractility unveils early Structural and Functional Pathology in iPSC Models of Hypertrophic Cardiomyopathy
    Article Snippet: Cell or tissue lysis and RNA recovery were performed using RNAqueous®-Micro Kit (Thermo Scientific). .. At least 320 ng RNA per sample was synthesized for cDNA using Super Script III Reverse Transcriptase Kit (Thermo Scientific). .. Gene expression was performed using Applied Biosystems Step One Plus Light Cycler instrument using SYBR green master mix (Thermo Scientific).

    Random Hexamer:

    Article Title: A maternally inherited Chromosomal Passenger Complex regulates germ plasm ribonucleoparticle aggregation in Zebrafish
    Article Snippet: .. Total RNA was extracted from pools of five wild-type embryos using Trizol (Invitrogen) according to manufacturer instructions. cDNA was synthesized using the Super Script III reverse transcriptase kit (Invitrogen) with a random hexamer primer. ..

    RNA Extraction:

    Article Title: miR-451a suppresses papillary thyroid cancer cell proliferation and invasion and facilitates apoptosis through targeting DCBLD2 and AKT1.
    Article Snippet: Papillary thyroid cancer (PTC) is a common malignancy.. MicroRNAs (miRNAs) may act as oncogenes or tumor suppressor genes.. However, the role of miR-451a in PTC is not fully understood.

    Isolation:

    Article Title: miR-451a suppresses papillary thyroid cancer cell proliferation and invasion and facilitates apoptosis through targeting DCBLD2 and AKT1.
    Article Snippet: Papillary thyroid cancer (PTC) is a common malignancy.. MicroRNAs (miRNAs) may act as oncogenes or tumor suppressor genes.. However, the role of miR-451a in PTC is not fully understood.

    Incubation:

    Article Title: Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients
    Article Snippet: Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc., USA), and RNA was quantified using a Nanodrop 200° ND-1000 UV–vis spectrophotometer (Nanodrop Technologies, USA). .. The reverse transcriptase reaction was performed using 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (Thermo Fisher Scientific, France) in a Thermal Cycler 2720 thermocycler (Applied Biosystems, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). .. PCR amplification was performed using an Absolute Blue SYBR Green Fluorescein Kit (Roche Molecular Biochemicals, France) and run on a StepOnePlus system (Life Technologies).

    other:

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    cDNA Synthesis:

    Article Title: Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells
    Article Snippet: The RNA amount and purity of each sample were quantified using NanoDrop (ND-1000, Wilmington, DE, USA). .. The mRNA was reverse transcribed into first-strand cDNA using the Super Script III reverse transcriptase kit (Invitrogen, 18,080–085), followed by second-strand cDNA synthesis using the Second Strand cDNA synthesis kit (Bio Wavelet, BWR001). ..



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