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stratascript rt rnase h reverse transcriptase  (Agilent technologies)


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    Agilent technologies stratascript rt rnase h reverse transcriptase
    Stratascript Rt Rnase H Reverse Transcriptase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stratascript+(h-reverse+transcriptase/pm09340009-88-65-72
    Average 90 stars, based on 1 article reviews
    stratascript rt rnase h reverse transcriptase - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: A potent synthetic nanobody targets RBD and protects mice from SARS-CoV-2 infection
    Article Snippet: Selected mRNAs were purified and 460 reverse-transcripted into single-chain DNA with the primer 5’-461 CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent).

    Article Title: A potent synthetic nanobody targets RBD and protects mice from SARS-CoV-2 infection
    Article Snippet: Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent).

    Article Title: Early adaptation to oxygen is key to the industrially important traits of Lactococcus lactis ssp. cremoris during milk fermentation
    Article Snippet: Ten μg of total RNA were labeled overnight at 42°C using 2 μl of Cy5-dCTP (Amersham Pharmacia), 4 μl of reverse transcriptase (AffinityScript, StrataGene) and 5 μg of random hexamers (Bioprime DNA labeling System, Invitrogen).

    SYBR Green Assay:

    Article Title: Genomic Flexibility of Human Endogenous Retrovirus Type K
    Article Snippet: Where indicated (see Fig. S1B in the supplemental material), DNase-free RNase (Roche) was added at a 1:10 dilution to extracted nucleic acids and incubated for 30 min at 37°C to eliminate RNA. .. The HERV-K viral load was quantitated using the Brilliant III ultrafast SYBR green quantitative reverse transcription-PCR (qRT-PCR) kit (Agilent) with or without the included reverse transcriptase and using either HERV-K gag -specific primers (GagRTF, 5′-AGCAGGTCAGGTGCCTGTAACATT-3′; GagRTR, 5′-TGGTGCCGTAGGATTAAGTCTCCT-3′) or env -specific primers (env1RTF, 5′-GATGGTAACACCAGTCAC-3′; env1RTR, 5′-GGCAAGGTTTCCCTTTAG-3′; env2RTF, 5′-AGACACCGCAATCGAGCACCGTTGA-3′; env2RTR, 5′-ATCAAGGCTGCAAGCAGCATACTC-3′). .. The Applied Biosystems StepOnePlus real-time PCR system was used to quantify viral load by the standard curve method.

    Reverse Transcription:

    Article Title: Genomic Flexibility of Human Endogenous Retrovirus Type K
    Article Snippet: Where indicated (see Fig. S1B in the supplemental material), DNase-free RNase (Roche) was added at a 1:10 dilution to extracted nucleic acids and incubated for 30 min at 37°C to eliminate RNA. .. The HERV-K viral load was quantitated using the Brilliant III ultrafast SYBR green quantitative reverse transcription-PCR (qRT-PCR) kit (Agilent) with or without the included reverse transcriptase and using either HERV-K gag -specific primers (GagRTF, 5′-AGCAGGTCAGGTGCCTGTAACATT-3′; GagRTR, 5′-TGGTGCCGTAGGATTAAGTCTCCT-3′) or env -specific primers (env1RTF, 5′-GATGGTAACACCAGTCAC-3′; env1RTR, 5′-GGCAAGGTTTCCCTTTAG-3′; env2RTF, 5′-AGACACCGCAATCGAGCACCGTTGA-3′; env2RTR, 5′-ATCAAGGCTGCAAGCAGCATACTC-3′). .. The Applied Biosystems StepOnePlus real-time PCR system was used to quantify viral load by the standard curve method.

    Article Title: Differences in Virulence among PVY Isolates of Different Geographical Origins When Infecting an Experimental Host under Two Growing Environments Are Not Determined by HCPro
    Article Snippet: Briefly, total RNA was extracted from three pooled discs of 1 cm diameter each of systemic leaf tissue for each tested plant using TRIzol reagent (Invitrogen) following the manufacturer’s instructions, and contaminant DNA was removed by treatment with a TURBO DNA-free kit (Ambion, Austin, TX, USA) as described [ , ]. .. RT-qPCR was performed using a final reaction volume of 15 uL that contained 7.5 μL of Brilliant III Ultra-Fast RT-qPCR Master Mix (Agilent, Santa Clara, CA, USA), 1.8 μL of RNase-free water, 0.75 μL of reverse transcriptase (Agilent), 0.15 μL of 100 mM dithiothreitol (Agilent), 0.3 μM each primer, and 3 μL of total RNA extract (approximately 15 ng RNA/μL). ..

    Article Title: High Temperature, High Ambient CO 2 Affect the Interactions between Three Positive-Sense RNA Viruses and a Compatible Host Differentially, but not Their Silencing Suppression Efficiencies
    Article Snippet: TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was used to extract total RNA, and DNA contaminants were removed by treatment with TURBO DNA-free kit (Ambion, Austin, TX, USA). .. A one-step real-time quantitative reverse transcription (RT-qPCR) was performed using 15 μl of a reaction mix that contained 7.5 μl of Brilliant III Ultra-Fast RT-qPCR Master Mix (Agilent, Santa Clara, CA, USA), 1.8 μl of RNase-free water, 0.75 μl of reverse transcriptase (Agilent), 0.15 μl of 100 mM dithiothreitol (Agilent), 0.3 μM each primer, and 3 μl of total RNA extract (approximately 10 ng RNA/μl). ..

    Article Title: HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification
    Article Snippet: The number of people living with HIV continues to increase with the current total near 38 million, of which about 26 million are receiving antiretroviral therapy (ART).. These treatment regimens are highly effective when properly managed, requiring routine viral load monitoring to assess successful viral suppression.. Efforts to expand access by decentralizing HIV nucleic acid testing in lowand middle-income countries (LMICs) has been hampered by the cost and complexity of current tests.

    Quantitative RT-PCR:

    Article Title: Differences in Virulence among PVY Isolates of Different Geographical Origins When Infecting an Experimental Host under Two Growing Environments Are Not Determined by HCPro
    Article Snippet: Briefly, total RNA was extracted from three pooled discs of 1 cm diameter each of systemic leaf tissue for each tested plant using TRIzol reagent (Invitrogen) following the manufacturer’s instructions, and contaminant DNA was removed by treatment with a TURBO DNA-free kit (Ambion, Austin, TX, USA) as described [ , ]. .. RT-qPCR was performed using a final reaction volume of 15 uL that contained 7.5 μL of Brilliant III Ultra-Fast RT-qPCR Master Mix (Agilent, Santa Clara, CA, USA), 1.8 μL of RNase-free water, 0.75 μL of reverse transcriptase (Agilent), 0.15 μL of 100 mM dithiothreitol (Agilent), 0.3 μM each primer, and 3 μL of total RNA extract (approximately 15 ng RNA/μL). ..

    Article Title: High Temperature, High Ambient CO 2 Affect the Interactions between Three Positive-Sense RNA Viruses and a Compatible Host Differentially, but not Their Silencing Suppression Efficiencies
    Article Snippet: TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was used to extract total RNA, and DNA contaminants were removed by treatment with TURBO DNA-free kit (Ambion, Austin, TX, USA). .. A one-step real-time quantitative reverse transcription (RT-qPCR) was performed using 15 μl of a reaction mix that contained 7.5 μl of Brilliant III Ultra-Fast RT-qPCR Master Mix (Agilent, Santa Clara, CA, USA), 1.8 μl of RNase-free water, 0.75 μl of reverse transcriptase (Agilent), 0.15 μl of 100 mM dithiothreitol (Agilent), 0.3 μM each primer, and 3 μl of total RNA extract (approximately 10 ng RNA/μl). ..

    Detection Assay:

    Article Title: HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification
    Article Snippet: The number of people living with HIV continues to increase with the current total near 38 million, of which about 26 million are receiving antiretroviral therapy (ART).. These treatment regimens are highly effective when properly managed, requiring routine viral load monitoring to assess successful viral suppression.. Efforts to expand access by decentralizing HIV nucleic acid testing in lowand middle-income countries (LMICs) has been hampered by the cost and complexity of current tests.

    Recombinase Polymerase Amplification:

    Article Title: HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification
    Article Snippet: The number of people living with HIV continues to increase with the current total near 38 million, of which about 26 million are receiving antiretroviral therapy (ART).. These treatment regimens are highly effective when properly managed, requiring routine viral load monitoring to assess successful viral suppression.. Efforts to expand access by decentralizing HIV nucleic acid testing in lowand middle-income countries (LMICs) has been hampered by the cost and complexity of current tests.



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