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20x ssc solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 20x ssc solution
    20x Ssc Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ssc+buffer+solution/SSC+buffer/pmc12706008-498-30-33
    Average 99 stars, based on 1 article reviews
    20x ssc solution - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Saline:

    Article Title: PhuS conformational dynamics are essential for DNA binding and heme-responsive control of the prrF operon in Pseudomonas aeruginosa
    Article Snippet: The qRT-PCR reactions were carried out on StepOne and QuantStudio3 (Applied Biosystems) qPCR machines using TaqMan FastStart Universal Probe Master Mix (Roche) according to manufacturer’s instructions and gene specific primers ( ). .. The membranes were then washed in 2X saline-sodium citrate (SSC) buffer (Invitrogen) for 5 min at 25 °C and RNA immobilized by UV crosslinking for 2 min. .. The membrane was prehybridized in ULTRAhyb Ultrasensitive Hybridization Buffer (ThermoFisher) for 30 min at 68 ̊C, followed by overnight hybridization at 42 ̊C with 0.1 nM PrrF1 or PrrF2 biotinylated northern blot probes.

    Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1
    Article Snippet: This was followed by a 30-min wash with 4,6-diamidino-2-phenylindole (DAPI) in wash buffer A to counterstain the nuclei (5 ng/ml; D9542 Sigma) at 37°C in the dark. .. The last wash was done with Stellaris wash buffer B (SMF-WB1-20, LGC Biosearch Technologies) at RT for 5 min. For signal enhancement, coverslips were equilibrated with Base Glucose Buffer composed of 2× saline-sodium citrate (SSC) buffer (1051520320× UltraPure Invitrogen , Fisher Scientific), 0.4% glucose solution (∼20% in H 2 O BioUltra, 49163, Sigma–Aldrich), and 20 mM RNase-free Tris pH 8.0 (1 M, 10259194, Fisher Scientific) in RNase-free H 2 O. .. Then, coverslips were incubated for 5 min in Base Glucose Buffer supplemented with a 1:100 dilution of glucose oxidase (3.7 mg/ml; G7141, Sigma–Aldrich) and catalase (4 mg/ml; C1345, Sigma–Aldrich).

    Article Title: A Novel Long Noncoding RNA – LNC000133 Associated With Steroid‐Induced Osteonecrosis of the Femoral Head Promotes Osteoblast Differentiation Through Bone Marrow Mesenchymal Stem Cells‐Derived Exosomes Pathway: A Bioinformatics Validation and Detailed Mechanistic Study
    Article Snippet: .. After hybridization, the membrane was washed twice at 42°C in 2× Saline‐Sodium Citrate (SSC) buffer with 0.1% SDS (Invitrogen Life Technologies, Carlsbad, CA) for 20 min each wash. Signal detection was achieved by exposing the membrane to Kodak XAR‐5 film (Sigma‐Aldrich) [ ]. ..

    Fluorescence In Situ Hybridization:

    Article Title: Direct targeting of C9ORF72 repeat RNA with fluorinated antisense oligonucleotides
    Article Snippet: DNA oligonucleotide fluorescent probe (G 2 C 4 ) 3 Alexa647 complementary to the expansion repeats of C9ORF72 gene was purchased from IDT. .. On the day of the FISH staining, cells were rinsed for 2 min in 2× SSC buffer (Thermo Fisher Scientific) with 15% formamide. ..

    Staining:

    Article Title: Direct targeting of C9ORF72 repeat RNA with fluorinated antisense oligonucleotides
    Article Snippet: DNA oligonucleotide fluorescent probe (G 2 C 4 ) 3 Alexa647 complementary to the expansion repeats of C9ORF72 gene was purchased from IDT. .. On the day of the FISH staining, cells were rinsed for 2 min in 2× SSC buffer (Thermo Fisher Scientific) with 15% formamide. ..

    Magnetic Beads:

    Article Title: On-site amplification-free electrochemical detection of the plant pathogen Xylella fastidiosa via cathodic potential-induced DNA adsorption.
    Article Snippet: Plant diseases pose a growing threat to global food security, with invasive bacterial pathogens presenting particular challenges for early detection and containment.. Xylella fastidiosa is among the most destructive of these pathogens, infecting hundreds of plant species and posing a severe biosecurity risk to agricultural systems, including those in Australia.. Despite its significance, routine detection still relies on laboratorybased molecular amplification methods that are slow, costly, and poorly suited to field deployment.

    Hybridization:

    Article Title: A Novel Long Noncoding RNA – LNC000133 Associated With Steroid‐Induced Osteonecrosis of the Femoral Head Promotes Osteoblast Differentiation Through Bone Marrow Mesenchymal Stem Cells‐Derived Exosomes Pathway: A Bioinformatics Validation and Detailed Mechanistic Study
    Article Snippet: .. After hybridization, the membrane was washed twice at 42°C in 2× Saline‐Sodium Citrate (SSC) buffer with 0.1% SDS (Invitrogen Life Technologies, Carlsbad, CA) for 20 min each wash. Signal detection was achieved by exposing the membrane to Kodak XAR‐5 film (Sigma‐Aldrich) [ ]. ..

    Membrane:

    Article Title: A Novel Long Noncoding RNA – LNC000133 Associated With Steroid‐Induced Osteonecrosis of the Femoral Head Promotes Osteoblast Differentiation Through Bone Marrow Mesenchymal Stem Cells‐Derived Exosomes Pathway: A Bioinformatics Validation and Detailed Mechanistic Study
    Article Snippet: .. After hybridization, the membrane was washed twice at 42°C in 2× Saline‐Sodium Citrate (SSC) buffer with 0.1% SDS (Invitrogen Life Technologies, Carlsbad, CA) for 20 min each wash. Signal detection was achieved by exposing the membrane to Kodak XAR‐5 film (Sigma‐Aldrich) [ ]. ..

    other:

    Article Title: Comparative analysis of flavivirus sfRNA dynamics and secondary structure
    Article Snippet: RNA was transferred to Brightstar-Plus Positively Charged Nylon membrane (Invitrogen, AM10102) at 300 mA for 35 minutes in 0.5x TBE in an Owl Hep1 Electroblotter (Thermo Scientific, HEP-1).

    Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer
    Article Snippet: After blocking with PBS containing 5% normal goat serum (MilliporeSigma) at room temperature for 1 h, the cells were incubated with the primary antibody (Anti-USP4; 1:500; cat. no. ab181105; Abcam) overnight at 4°C, followed by incubation with Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody (1:500; cat. no. A11008; Thermo Fisher Scientific, Inc.) for 1 h at room temperature in the dark.



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