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visium spatial gene expression profiles spatial transcriptome analysis  (10X Genomics)

 
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    Structured Review

    10X Genomics visium spatial gene expression profiles spatial transcriptome analysis
    Visium Spatial Gene Expression Profiles Spatial Transcriptome Analysis, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spatial+transcriptomic+profiling+visium/data+spatial+transcriptomic/pm39632980-245-3-12
    Average 86 stars, based on 1 article reviews
    visium spatial gene expression profiles spatial transcriptome analysis - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Spatial Transcriptomics:

    Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
    Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

    Sequencing:

    Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
    Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

    Article Title: Spatial single-cell landscape of tumor-associated macrophages and their crosstalk with the tumor microenvironment.
    Article Snippet: .. To minimize batch effects caused by differences in sequencing platforms and methodologies, all single-cell and spatial transcriptomic data were obtained exclusively from the 10x Genomics and 10x Visium platforms. ..

    Gene Expression:

    Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
    Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

    Single Cell:

    Article Title: Spatial single-cell landscape of tumor-associated macrophages and their crosstalk with the tumor microenvironment.
    Article Snippet: .. To minimize batch effects caused by differences in sequencing platforms and methodologies, all single-cell and spatial transcriptomic data were obtained exclusively from the 10x Genomics and 10x Visium platforms. ..

    Article Title: The Role of Tumor Necrosis Factor Signaling in Atherosclerosis and Stroke
    Article Snippet: .. To characterise TNF signaling within atherosclerotic plaques, we analysed two publicly available datasets: (i) an integrated single-cell RNA-sequencing (scRNA-seq) atlas of 259,116 cells from human carotid, coronary, and femoral plaques (73 donors), and (ii) Xenium (10x Genomics) spatial transcriptomic data comprising 120,164 cells from carotid endarterectomy specimens with pathologist-annotated subregions (12 donors). ..

    In Situ:

    Article Title: SARS-CoV-2 infection and vaccination elicit distinct pharyngeal mucosal B cell responses in children.
    Article Snippet: .. Spatial transcriptomic profiling with Xenium In Situ platform Slides were prepared following the manufacturer’s instructions and workflow for FFPE tissue samples (CG000578 Rev A; 10x Genomics). .. A 5-μm section from the tissue block containing the same paired tonsil and adenoid samples (one from INF donor and one from VAC donor) used for immunofluorescence were carefully attached to the sample area on a Xenium slide (Histoserv, MD).

    Article Title: An antioxidant therapy elicits distinct transcriptome responses in 22q11-deleted upper layer cortical projection neurons.
    Article Snippet: .. To assess L 2/3 PN transcriptional responses that underlie NAC’s therapeutic effects in vivo, we first established that spatial transcriptomic RNA quantification in situ (10X Genomics Xenium) securely identifies L 2/3 PNs and their neighbors, thus ensuring that transcriptional states can be assessed in intact cortices of early post-natal WT, LgDel, LgDel + NAC and WT + NAC L 2/3 mice. ..

    Article Title: Won't you be my neighbor? Control of the immune response by stromal and immune cell microenvironments within the lymph node.
    Article Snippet: Efficacious immune responses require the coordinated encounter of rare antigen-specific adaptive lymphocytes with their cognate innate antigen-presenting cells (APCs) in space and time.. This spatiotemporal problem of immunity is solved by secondary lymphoid organs, such as lymph nodes (LNs), which coordinate adaptive immune responses by recruiting APCs and lymphocytes into close juxtaposition with tissue antigens drained from the periphery.. A central tenet to the overall function of the LN is the spatial organization of leukocytes into discrete microenvironments orchestrated by the mesenchymal and endothelial cells, collectively termed LN stromal cells (LNSCs).

    Formalin-fixed Paraffin-Embedded:

    Article Title: SARS-CoV-2 infection and vaccination elicit distinct pharyngeal mucosal B cell responses in children.
    Article Snippet: .. Spatial transcriptomic profiling with Xenium In Situ platform Slides were prepared following the manufacturer’s instructions and workflow for FFPE tissue samples (CG000578 Rev A; 10x Genomics). .. A 5-μm section from the tissue block containing the same paired tonsil and adenoid samples (one from INF donor and one from VAC donor) used for immunofluorescence were carefully attached to the sample area on a Xenium slide (Histoserv, MD).

    In Vivo:

    Article Title: An antioxidant therapy elicits distinct transcriptome responses in 22q11-deleted upper layer cortical projection neurons.
    Article Snippet: .. To assess L 2/3 PN transcriptional responses that underlie NAC’s therapeutic effects in vivo, we first established that spatial transcriptomic RNA quantification in situ (10X Genomics Xenium) securely identifies L 2/3 PNs and their neighbors, thus ensuring that transcriptional states can be assessed in intact cortices of early post-natal WT, LgDel, LgDel + NAC and WT + NAC L 2/3 mice. ..

    RNA sequencing:

    Article Title: The Role of Tumor Necrosis Factor Signaling in Atherosclerosis and Stroke
    Article Snippet: .. To characterise TNF signaling within atherosclerotic plaques, we analysed two publicly available datasets: (i) an integrated single-cell RNA-sequencing (scRNA-seq) atlas of 259,116 cells from human carotid, coronary, and femoral plaques (73 donors), and (ii) Xenium (10x Genomics) spatial transcriptomic data comprising 120,164 cells from carotid endarterectomy specimens with pathologist-annotated subregions (12 donors). ..



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    10X Genomics visium spatial gene expression profiles spatial transcriptome analysis
    Visium Spatial Gene Expression Profiles Spatial Transcriptome Analysis, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spatial+transcriptomic+profiling+visium/data+spatial+transcriptomic/pm39632980-245-3-12
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    90
    10X Genomics spatial transcription dataset fresh frozen visium on cytassist: humanbreast cancer, probe-based whole transcriptome profiling
    Identifying npCR-associated fibroblasts by integrating proteomics and single-cell sequencing data. (A) Volcano plot of differentially expressed proteins between the pCR and npCR groups. (B) Upregulated pathways in the npCR group based on GO term enrichment analysis of differentially expressed proteins. (C–D) Uniform Manifold Approximation and Projection (UMAP) of major cell populations in our study, different colored dots represent different cell types. (E–F) The pCR and npCR phenotypes were mapped from proteomics to scRNA-seq data. (G) Pseudotime trajectories of fibroblast. (H–I) The GO/KEGG enrichment based on the GSVA algorithm. (J) <t>Transcription</t> factor regulation of npCR-related fibroblast compared with other fibroblast. (K–L) The intercellular cross-talk analysis between fibroblast and other cells in TME.
    Spatial Transcription Dataset Fresh Frozen Visium On Cytassist: Humanbreast Cancer, Probe Based Whole Transcriptome Profiling, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spatial+transcriptomic+profiling+visium/spatial+transcription+dataset+fresh+frozen+visium+on+cytassist++humanbreast+cancer++probe+based+whole+transcriptome+profiling/pmc11254208-140-1-18
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    Image Search Results


    Identifying npCR-associated fibroblasts by integrating proteomics and single-cell sequencing data. (A) Volcano plot of differentially expressed proteins between the pCR and npCR groups. (B) Upregulated pathways in the npCR group based on GO term enrichment analysis of differentially expressed proteins. (C–D) Uniform Manifold Approximation and Projection (UMAP) of major cell populations in our study, different colored dots represent different cell types. (E–F) The pCR and npCR phenotypes were mapped from proteomics to scRNA-seq data. (G) Pseudotime trajectories of fibroblast. (H–I) The GO/KEGG enrichment based on the GSVA algorithm. (J) Transcription factor regulation of npCR-related fibroblast compared with other fibroblast. (K–L) The intercellular cross-talk analysis between fibroblast and other cells in TME.

    Journal: International Journal of Surgery (London, England)

    Article Title: Integrative analysis identifies molecular features of fibroblast and the significance of fibrosis on neoadjuvant chemotherapy response in breast cancer

    doi: 10.1097/JS9.0000000000001360

    Figure Lengend Snippet: Identifying npCR-associated fibroblasts by integrating proteomics and single-cell sequencing data. (A) Volcano plot of differentially expressed proteins between the pCR and npCR groups. (B) Upregulated pathways in the npCR group based on GO term enrichment analysis of differentially expressed proteins. (C–D) Uniform Manifold Approximation and Projection (UMAP) of major cell populations in our study, different colored dots represent different cell types. (E–F) The pCR and npCR phenotypes were mapped from proteomics to scRNA-seq data. (G) Pseudotime trajectories of fibroblast. (H–I) The GO/KEGG enrichment based on the GSVA algorithm. (J) Transcription factor regulation of npCR-related fibroblast compared with other fibroblast. (K–L) The intercellular cross-talk analysis between fibroblast and other cells in TME.

    Article Snippet: The spatial transcription dataset (Fresh Frozen Visium on CytAssist: HumanBreast Cancer, Probe-Based Whole Transcriptome Profiling) was retrieved from 10X Genomics ( https://www.10xgenomics.com/ ).

    Techniques: Sequencing