Review




Structured Review

Bio-Rad pellet
Pellet, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us11951159-296-1-32
Average 93 stars, based on 114 article reviews
pellet - by Bioz Stars, 2026-09
93/100 stars

Images

Related Articles

Centrifugation:

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: The pellet was washed three times using Reagent 1 plus FOCUS ProteaseArrest (G-Bioscience) and centrifuged for 30 minutes at 16,100 g at room temperature. .. The supernatant was discarded after each centrifugation and, after the final wash, the pellet was resuspended with Reagent 2 (8 M urea, 4% w/v CHAPS, 40 mM Tris, 0.2% w/v Bio-Lyte 3/10 ampholyte)+tributyl phosphine (TBP) (ReadyPrep Sequential Extraction Kit) (Bio-Rad), followed by incubation at room temperature for 1 hour shaking at 225 rpm and centrifugation as previously described. ..

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: .. The pellet was resuspended with Reagent 3 (5 M urea, 2 M thiourea, 2% w/v CHAPS, 2% w/v SB 3-10, 40 mM Tris, 0.2% w/v Bio-Lyte 3/10 ampholyte)+TBP (ReadyPrep Sequential Extraction Kit, Bio-Rad) followed by incubation and centrifugation as described above. ..

Extraction:

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: The pellet was washed three times using Reagent 1 plus FOCUS ProteaseArrest (G-Bioscience) and centrifuged for 30 minutes at 16,100 g at room temperature. .. The supernatant was discarded after each centrifugation and, after the final wash, the pellet was resuspended with Reagent 2 (8 M urea, 4% w/v CHAPS, 40 mM Tris, 0.2% w/v Bio-Lyte 3/10 ampholyte)+tributyl phosphine (TBP) (ReadyPrep Sequential Extraction Kit) (Bio-Rad), followed by incubation at room temperature for 1 hour shaking at 225 rpm and centrifugation as previously described. ..

Article Title: Is Melanoma a stem cell tumor? Identification of neurogenic proteins in trans-differentiated cells
Article Snippet: .. Each cell pellet was washed again 2× with PBS and proteins were extracted sequentially by a modified two-step solubilization procedure using two reagents containing different concentrations of 5–8 M Urea, 2%–4% CHAPS, 2 M thiourea and other non-ionic and/zwitterionic detergents (BioRad Sequential extraction kit CA, USA). ..

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: .. The pellet was resuspended with Reagent 3 (5 M urea, 2 M thiourea, 2% w/v CHAPS, 2% w/v SB 3-10, 40 mM Tris, 0.2% w/v Bio-Lyte 3/10 ampholyte)+TBP (ReadyPrep Sequential Extraction Kit, Bio-Rad) followed by incubation and centrifugation as described above. ..

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: .. Aqueous soluble proteins were extracted by adding Reagent 1 (40 mM Tris) (ReadyPrep Sequential Extraction Kit; Bio-Rad, Hercules, California, USA) and FOCUS ProteaseArrest (G-Bioscience; St. Louis, Missouri, USA) to the samples and grinding with a polytron (PCU-2-110) (Brinkmann Instruments; Westbury, New York, USA) for 10 seconds, returned to ice for 30 seconds, repeating this twice. .. Ribonuclease A from Bovine Pancreas (Sigma Aldrich, St. Louis, Missouri, USA) was added and the samples incubated at room temperature for 45 minutes on an orbital shaker at 225 rpm.

Article Title: Horn fly protein as active antigen in anti-horn fly vaccine for protection of bovines against horn fly infestations
Article Snippet: .. The adult flies used in the study were from the reference laboratory strain that has been kept in colony feeding upon citrated bovine blood at the USDA-ARS Knipling-Bushland U.S. Livestock Research Laboratory (KBUSLIRL) for over 50 years (Lohmeyer & Kammlah, Southwest Entomol., (2006) 31:83-5) Aqueous- and urea-extractible proteins were extracted using the ReadyPrep Sequential Extraction Kit (Bio-Rad) following the manufacturer's recommendation with a few modifications. ..

Article Title: Improving the genome and proteome annotations of the marine model diatom Thalassiosira pseudonana using a proteogenomics strategy.
Article Snippet: .. The first method used a Sequential Extraction Kit (Cat No. 1632100, BioRad) to extract proteins using reagents in three steps according to protein solubility. ..

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: .. To determine if the selected tick proteins for evaluation as vaccine candidates could be detected in tick tissues, aqueous- and urea-extractible proteins were extracted using the ReadyPrep Sequential Extraction Kit (Bio-Rad) following the manufacturer's recommendation with a few modifications. ..

Incubation:

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: The pellet was washed three times using Reagent 1 plus FOCUS ProteaseArrest (G-Bioscience) and centrifuged for 30 minutes at 16,100 g at room temperature. .. The supernatant was discarded after each centrifugation and, after the final wash, the pellet was resuspended with Reagent 2 (8 M urea, 4% w/v CHAPS, 40 mM Tris, 0.2% w/v Bio-Lyte 3/10 ampholyte)+tributyl phosphine (TBP) (ReadyPrep Sequential Extraction Kit) (Bio-Rad), followed by incubation at room temperature for 1 hour shaking at 225 rpm and centrifugation as previously described. ..

Article Title: Cattle fever tick-infestation vaccines and uses thereof
Article Snippet: .. The pellet was resuspended with Reagent 3 (5 M urea, 2 M thiourea, 2% w/v CHAPS, 2% w/v SB 3-10, 40 mM Tris, 0.2% w/v Bio-Lyte 3/10 ampholyte)+TBP (ReadyPrep Sequential Extraction Kit, Bio-Rad) followed by incubation and centrifugation as described above. ..

Modification:

Article Title: Is Melanoma a stem cell tumor? Identification of neurogenic proteins in trans-differentiated cells
Article Snippet: .. Each cell pellet was washed again 2× with PBS and proteins were extracted sequentially by a modified two-step solubilization procedure using two reagents containing different concentrations of 5–8 M Urea, 2%–4% CHAPS, 2 M thiourea and other non-ionic and/zwitterionic detergents (BioRad Sequential extraction kit CA, USA). ..

Solubility:

Article Title: Improving the genome and proteome annotations of the marine model diatom Thalassiosira pseudonana using a proteogenomics strategy.
Article Snippet: .. The first method used a Sequential Extraction Kit (Cat No. 1632100, BioRad) to extract proteins using reagents in three steps according to protein solubility. ..



Similar Products

93
Bio-Rad readyprep sequential extraction kit
Readyprep Sequential Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us12274741-290-26-30
Average 93 stars, based on 1 article reviews
readyprep sequential extraction kit - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Bio-Rad urea buffer
Urea Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit+Reagent+2/pmc11257952-255-18-20
Average 93 stars, based on 1 article reviews
urea buffer - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Bio-Rad pellet
Pellet, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us11951159-296-1-32
Average 93 stars, based on 1 article reviews
pellet - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Bio-Rad aqueous soluble proteins
Aqueous Soluble Proteins, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/us11951159-290-0-16
Average 93 stars, based on 1 article reviews
aqueous soluble proteins - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

97
Bio-Rad ready prep sequential extraction kit
Ready Prep Sequential Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/Lysis+Buffer/pmc10322688-255-21-28
Average 97 stars, based on 1 article reviews
ready prep sequential extraction kit - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier

93
Bio-Rad sequential extraction kit
Sequential Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequential+extraction+kit/ReadyPrep+Sequential+Extraction+Kit/pm37073328-170-5-11
Average 93 stars, based on 1 article reviews
sequential extraction kit - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results