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Ridom GmbH seqsphere+ software
Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom <t>Seqsphere</t> + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site
Seqsphere+ Software, supplied by Ridom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/seqsphere++software/seqsphere/pmc12278488-119-12-15
Average 90 stars, based on 1 article reviews
seqsphere+ software - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering"

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering

Journal: BMC Microbiology

doi: 10.1186/s12866-025-04152-8

Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site
Figure Legend Snippet: Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site

Techniques Used: Construct, Generated, Isolation, Plasmid Preparation

Related Articles

Construct:

Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections.
Article Snippet: Genome processing was performed on the BV-BRC platform (https://www.bvbrc.org/) with Ridom SeqSphere + v9.0 software (Münster, Germany).

Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022)
Article Snippet: MLST and cgMLST were carried out using established typing schemes in Ridom SeqSphere+ v9.0.10 ( , ).

Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background.
Article Snippet: Sequences were trimmed to an average Phred quality score of 30 in 20 bp windows, assembled using optimized k- mer size, downsampled to 120× using version 1.1.04 of Velvet, and assigned multilocus sequence type (ST), clonal complex (CC), and core genome multilocus sequence typing (cgMLST) complex type [13] using version 7.2.0 of SeqSphere+ (Ridom GmbH, Münster, Germany).

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape [ ].

Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management.
Article Snippet: The resulting genome sequences were analysed using Ridom SeqSphere (v8 4.1).

Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine.
Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 (Ridom, Germany) using the cgMLST schemes developed for P. aeruginosa [36] and A. baumannii [37] with a cutoff of 90%.

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Core-genome MLST (cgMLST) analysis for 286 R. equi genomes was performed in Ridom Seqsphere+ (v9.0.1; Ridom GmbH, Münster, Germany).

Generated:

Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections.
Article Snippet: Genome processing was performed on the BV-BRC platform (https://www.bvbrc.org/) with Ridom SeqSphere + v9.0 software (Münster, Germany).

Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022)
Article Snippet: MLST and cgMLST were carried out using established typing schemes in Ridom SeqSphere+ v9.0.10 ( , ).

Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background.
Article Snippet: Sequences were trimmed to an average Phred quality score of 30 in 20 bp windows, assembled using optimized k- mer size, downsampled to 120× using version 1.1.04 of Velvet, and assigned multilocus sequence type (ST), clonal complex (CC), and core genome multilocus sequence typing (cgMLST) complex type [13] using version 7.2.0 of SeqSphere+ (Ridom GmbH, Münster, Germany).

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape [ ].

Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management.
Article Snippet: The resulting genome sequences were analysed using Ridom SeqSphere (v8 4.1).

Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine.
Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 (Ridom, Germany) using the cgMLST schemes developed for P. aeruginosa [36] and A. baumannii [37] with a cutoff of 90%.

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Core-genome MLST (cgMLST) analysis for 286 R. equi genomes was performed in Ridom Seqsphere+ (v9.0.1; Ridom GmbH, Münster, Germany).

Isolation:

Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections.
Article Snippet: Genome processing was performed on the BV-BRC platform (https://www.bvbrc.org/) with Ridom SeqSphere + v9.0 software (Münster, Germany).

Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022)
Article Snippet: MLST and cgMLST were carried out using established typing schemes in Ridom SeqSphere+ v9.0.10 ( , ).

Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background.
Article Snippet: Sequences were trimmed to an average Phred quality score of 30 in 20 bp windows, assembled using optimized k- mer size, downsampled to 120× using version 1.1.04 of Velvet, and assigned multilocus sequence type (ST), clonal complex (CC), and core genome multilocus sequence typing (cgMLST) complex type [13] using version 7.2.0 of SeqSphere+ (Ridom GmbH, Münster, Germany).

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape [ ].

Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management.
Article Snippet: The resulting genome sequences were analysed using Ridom SeqSphere (v8 4.1).

Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine.
Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 (Ridom, Germany) using the cgMLST schemes developed for P. aeruginosa [36] and A. baumannii [37] with a cutoff of 90%.

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Core-genome MLST (cgMLST) analysis for 286 R. equi genomes was performed in Ridom Seqsphere+ (v9.0.1; Ridom GmbH, Münster, Germany).

Plasmid Preparation:

Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections.
Article Snippet: Genome processing was performed on the BV-BRC platform (https://www.bvbrc.org/) with Ridom SeqSphere + v9.0 software (Münster, Germany).

Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022)
Article Snippet: MLST and cgMLST were carried out using established typing schemes in Ridom SeqSphere+ v9.0.10 ( , ).

Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background.
Article Snippet: Sequences were trimmed to an average Phred quality score of 30 in 20 bp windows, assembled using optimized k- mer size, downsampled to 120× using version 1.1.04 of Velvet, and assigned multilocus sequence type (ST), clonal complex (CC), and core genome multilocus sequence typing (cgMLST) complex type [13] using version 7.2.0 of SeqSphere+ (Ridom GmbH, Münster, Germany).

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape [ ].

Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management.
Article Snippet: The resulting genome sequences were analysed using Ridom SeqSphere (v8 4.1).

Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine.
Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 (Ridom, Germany) using the cgMLST schemes developed for P. aeruginosa [36] and A. baumannii [37] with a cutoff of 90%.

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Article Snippet: Core-genome MLST (cgMLST) analysis for 286 R. equi genomes was performed in Ridom Seqsphere+ (v9.0.1; Ridom GmbH, Münster, Germany).



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Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom <t>Seqsphere</t> + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site
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Image Search Results


Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site

Journal: BMC Microbiology

Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering

doi: 10.1186/s12866-025-04152-8

Figure Lengend Snippet: Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site

Article Snippet: Core-genome MLST (cgMLST) analysis for 286 R. equi genomes was performed in Ridom Seqsphere+ (v9.0.1; Ridom GmbH, Münster, Germany).

Techniques: Construct, Generated, Isolation, Plasmid Preparation