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semiconductor-based influenza a microarray  (CombiMatrix)

 
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    CombiMatrix semiconductor-based influenza a microarray
    Semiconductor Based Influenza A Microarray, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semiconductor-based+microarray/influenza+microarrays/pm27077877-156-4-7
    Average 90 stars, based on 1 article reviews
    semiconductor-based influenza a microarray - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Current Approaches for Diagnosis of Influenza Virus Infections in Humans
    Article Snippet: A semiconductor-based Influenza A microarray developed by CombiMatrix Corporation (Irvine, CA, USA) has been shown to detect all known subtypes of influenza A viruses in less than five hours [ , ].

    Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay.
    Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [28].

    Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay
    Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

    Article Title: Detection Methods of Human and Animal Influenza Virus—Current Trends
    Article Snippet: CombiMatrix Corporation developed influenza A microarray detecting all known so-far virus subtypes in less than 5 h [ ].

    Article Title: Current Approaches for Diagnosis of Influenza Virus Infections in Humans.
    Article Snippet: A semiconductor-based Influenza A microarray developed by CombiMatrix Corporation (Irvine, CA, USA) has been shown to detect all known subtypes of influenza A viruses in less than five hours [57,58].

    Purification:

    Article Title: Enhancement of deoxyribonucleic acid microarray performance using post-hybridization signal amplification.
    Article Snippet: Microarray performance depends upon the ability to screen samples against a vast array of probes with the appropriate sensitivity and selectivity.. While these factors are significantly influenced by probe design, they are also subject to the particular detection methodology and reagents employed.. Herein we describe the incorporation of super avidin–biotin system (SABS) and secondary enzymatic enhancement (SEE) as post-hybridization signal amplification techniques to improve the sensitivity of oligonucleotide microareywords: ombiMatrix icroarray lectrochemical detection uper avidin–biotin system econdary enzymatic enhancement rays.

    Hybridization:

    Article Title: Enhancement of deoxyribonucleic acid microarray performance using post-hybridization signal amplification.
    Article Snippet: Microarray performance depends upon the ability to screen samples against a vast array of probes with the appropriate sensitivity and selectivity.. While these factors are significantly influenced by probe design, they are also subject to the particular detection methodology and reagents employed.. Herein we describe the incorporation of super avidin–biotin system (SABS) and secondary enzymatic enhancement (SEE) as post-hybridization signal amplification techniques to improve the sensitivity of oligonucleotide microareywords: ombiMatrix icroarray lectrochemical detection uper avidin–biotin system econdary enzymatic enhancement rays.



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    Image Search Results


    Simplified schematic overview of the gold nanoparticle (NP)-based microarray assay . The target is whole viral genome RNA (blue line), end modified capture oligos are 40 bp in length (black line) printed on the amino-modified glass slide and intermediate oligos are 65 bp in length (purple line) including 25-mer poly (A) tail (red line). The probe is 15 nm gold nanoparticles (NP) functionalized with poly dT tail (green line). The specific hybridizations between the capture oligo, RNA (or DNA) and intermediate oligo take place to form sandwich complexes in Step 1, then the gold NP probes bind to the complexes in Step 2, and silver staining is in Step 3.

    Journal: BMC Biotechnology

    Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

    doi: 10.1186/1472-6750-10-74

    Figure Lengend Snippet: Simplified schematic overview of the gold nanoparticle (NP)-based microarray assay . The target is whole viral genome RNA (blue line), end modified capture oligos are 40 bp in length (black line) printed on the amino-modified glass slide and intermediate oligos are 65 bp in length (purple line) including 25-mer poly (A) tail (red line). The probe is 15 nm gold nanoparticles (NP) functionalized with poly dT tail (green line). The specific hybridizations between the capture oligo, RNA (or DNA) and intermediate oligo take place to form sandwich complexes in Step 1, then the gold NP probes bind to the complexes in Step 2, and silver staining is in Step 3.

    Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

    Techniques: Microarray, Modification, Silver Staining

    Image from a Verigene ID™ detection system . (A). Microarray layout with positive control capture (closed red circles on the top), negative control which uses printing buffer as capture (closed yellow circles), M, H5, and N1 gene captures (filled as variable color of closed circles, spotted in triplicate) are indicated. (B). The light shades represent strong silver staining signals. A portion of the microarray images for DNA oligonucleotide following hybridization with PCR fragment are shown and light shades represent greater silver intensities for the M and H5 genes. The spots at the top of each array are positive controls. (C). Silver staining image for individual or multiple PCR products are shown and labeled. (D). Typical genomic microarray silver staining image from 0.2 μg of H1N1, H3N2 or H5N1 viral RNA are shown. The boxed areas highlight hits for specific subtypes.

    Journal: BMC Biotechnology

    Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

    doi: 10.1186/1472-6750-10-74

    Figure Lengend Snippet: Image from a Verigene ID™ detection system . (A). Microarray layout with positive control capture (closed red circles on the top), negative control which uses printing buffer as capture (closed yellow circles), M, H5, and N1 gene captures (filled as variable color of closed circles, spotted in triplicate) are indicated. (B). The light shades represent strong silver staining signals. A portion of the microarray images for DNA oligonucleotide following hybridization with PCR fragment are shown and light shades represent greater silver intensities for the M and H5 genes. The spots at the top of each array are positive controls. (C). Silver staining image for individual or multiple PCR products are shown and labeled. (D). Typical genomic microarray silver staining image from 0.2 μg of H1N1, H3N2 or H5N1 viral RNA are shown. The boxed areas highlight hits for specific subtypes.

    Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

    Techniques: Microarray, Positive Control, Negative Control, Silver Staining, Hybridization, Labeling

    Results of genomic array detection of influenza A viruses with specific captures

    Journal: BMC Biotechnology

    Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

    doi: 10.1186/1472-6750-10-74

    Figure Lengend Snippet: Results of genomic array detection of influenza A viruses with specific captures

    Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

    Techniques:

    Resequencing microarray output for identification and characterisation of the 12 influenza A viral RNAs

    Journal: BMC Genomics

    Article Title: Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm

    doi: 10.1186/1471-2164-11-586

    Figure Lengend Snippet: Resequencing microarray output for identification and characterisation of the 12 influenza A viral RNAs

    Article Snippet: Recently, Combimatrix Corporation (Mukilteo, WA) developed a semiconductor-based microarray and an integrated microfluidic array enabling the detection of 15 HA and 9 NA [ , ].

    Techniques: Microarray

    Avian sequences reconstructed by the PathogenID v2.0 and BLASTN alignment

    Journal: BMC Genomics

    Article Title: Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm

    doi: 10.1186/1471-2164-11-586

    Figure Lengend Snippet: Avian sequences reconstructed by the PathogenID v2.0 and BLASTN alignment

    Article Snippet: Recently, Combimatrix Corporation (Mukilteo, WA) developed a semiconductor-based microarray and an integrated microfluidic array enabling the detection of 15 HA and 9 NA [ , ].

    Techniques: Sequencing, Microarray