semi quantitative analysis (Bio-Rad)
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semi quantitative analysis
Semi Quantitative Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/semi+quantitative+analysis/Image+Lab+Software/pm39799997-151-6-13
Average 99 stars, based on 35737 article reviews
Semi Quantitative Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/semi+quantitative+analysis/Image+Lab+Software/pm39799997-151-6-13
Average 99 stars, based on 35737 article reviews
semi quantitative analysis - by Bioz Stars,
2026-11
99/100 stars
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other:Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals Article Snippet: After washing and incubating with an appropriate Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on Software:Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression Article Snippet: The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution. .. Band intensities were quantified using Article Title: Curcumin-Induced Vacuolizing Stress Reveals a Vulnerability Associated with NF1 Loss in Schwann Cells. Article Snippet: Membranes were probed with the following antibodies: anti-NF1 #sc-376886 (1:200) by Santa Cruz Biotechnology (Dallas, TX, USA), anti-PARP #10872500 (1:1000) by Roche, anti-NRF2 #PA58804 (1:1000) by Life Technologies, anti-TRX #EPR6110 (1:1000) by Abcam (Cambridge, UK), anti-SOD1 #TA50495 (1:1000) by OriGene Technologies, (Rockville, MD, USA), anti-cleaved caspase-3 (Asp175) #9661 (1:1000) by Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH-HRP #MA5-15738-HRP (1:5000) by Life Technologies. .. Bound antibodies were detected by chemiluminescence using WesternBright ECL (#K12045D50 Advansta, San Jose, CA, USA), and band intensities were quantified by densitometry with Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores. Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Staining:Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores. Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the Imaging:Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores. Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Incubation:Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using |
