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Yeasen Biotechnology hifair iii reverse transcriptase
Hifair Iii Reverse Transcriptase, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase/1st+cdna+for+hifair+iii+qpcr+strand+supermix+synthesis/pm42263544-125-7-11
Average 86 stars, based on 1 article reviews
hifair iii reverse transcriptase - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Reverse Transcription:

Article Title: Placenta-derived small extracellular vesicles transfer miR-520d-5p to mediate pancreatic β-cell injury in gestational diabetes mellitus by targeting SIRT1.
Article Snippet: The concentration and purity of the RNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. Subsequently, 1 μg of total RNA was reverse-transcribed into complementary DNA (cDNA) using the Hifair AdvanceFast 1st Strand cDNA Synthesis SuperMix (Yeasen, China). .. For miRNA analysis, cDNA was synthesized using the Hifair miRNA 1st Strand cDNA Synthesis Kit (Yeasen), following the manufacturer’s protocol.

Article Title: A Heterogeneously structured Mg-Zn laminate alloy for gradient degradation behavior
Article Snippet: RNA was quantified by nanodrop (NanoDrop2000c, Thermo Scientific, USA). .. Complementary DNA (cDNA) was synthesized with Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN Biotechnology (Shanghai, China), and dNTPs using SuperScript III Reverse Transcriptase (Life Technologies, USA). .. Predesigned and validated primers were purchased from Sangon Biotech ( ).

Article Title: ACOT1-specific expression modulates metabolic reprogramming in diabetic cardiomyopathy: The role of SREBP1c lactylation in CD36-mediated lipotoxicity
Article Snippet: RNA concentration and purity were assessed spectrophotometrically (NanoDrop, Thermo Fisher Scientific, USA). .. For reverse transcription, 1 μg of total RNA was converted to complementary DNA (cDNA) using Hifair III First Strand cDNA Synthesis SuperMix (YEASEN, Shanghai, China).Quantitative real-time PCR (qPCR) was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad, California, USA) using SYBR Green Master Mix (YEASEN). ..

cDNA Synthesis:

Article Title: Placenta-derived small extracellular vesicles transfer miR-520d-5p to mediate pancreatic β-cell injury in gestational diabetes mellitus by targeting SIRT1.
Article Snippet: The concentration and purity of the RNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. Subsequently, 1 μg of total RNA was reverse-transcribed into complementary DNA (cDNA) using the Hifair AdvanceFast 1st Strand cDNA Synthesis SuperMix (Yeasen, China). .. For miRNA analysis, cDNA was synthesized using the Hifair miRNA 1st Strand cDNA Synthesis Kit (Yeasen), following the manufacturer’s protocol.

Article Title: Dendrobine alleviates lung injury in septic mice by inhibiting mitochondrial-endoplasmic reticulum crosstalk-mediated NLRP3 inflammasome activation.
Article Snippet: EZ-press RNA purification kit (B0004D) was purchased from EZ Bioscience (California, USA).Lactic acid (LA) content assay kit (BC2235) was purchased from Solarbio (Beijing, China). .. Hifair® III 1st Strand cDNA Synthesis SuperMix (11120ES60), Hieff UNICON Universal Blue qPCR SYBR Green Master Mix (11184ES08), rProtein G agarose resin (36405ES08), and Rhod-2, AM (40776ES72) were purchased from Yeasen Biotechnology (Shanghai, China). .. Antibodies for NLRP3 (15101S), cleaved caspase-1 (4199S), HIF-1α (14179S), HK2 (2867S), and VDAC1 (4866S) were purchased from Cell Signaling Technology (Boston, MA, USA).

Article Title: A Heterogeneously structured Mg-Zn laminate alloy for gradient degradation behavior
Article Snippet: RNA was quantified by nanodrop (NanoDrop2000c, Thermo Scientific, USA). .. Complementary DNA (cDNA) was synthesized with Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN Biotechnology (Shanghai, China), and dNTPs using SuperScript III Reverse Transcriptase (Life Technologies, USA). .. Predesigned and validated primers were purchased from Sangon Biotech ( ).

Article Title: ACOT1-specific expression modulates metabolic reprogramming in diabetic cardiomyopathy: The role of SREBP1c lactylation in CD36-mediated lipotoxicity
Article Snippet: RNA concentration and purity were assessed spectrophotometrically (NanoDrop, Thermo Fisher Scientific, USA). .. For reverse transcription, 1 μg of total RNA was converted to complementary DNA (cDNA) using Hifair III First Strand cDNA Synthesis SuperMix (YEASEN, Shanghai, China).Quantitative real-time PCR (qPCR) was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad, California, USA) using SYBR Green Master Mix (YEASEN). ..

Article Title: Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli
Article Snippet: Total RNA was isolated from samples using the EASYspinPLus Bacterial RNA Rapid Extraction Kit (Aidlab, China). .. The cDNA was synthesized using Hifair® II 1st Strand cDNA Synthesis SuperMix for qPCR (Yeasen Biotechnology, China). .. Quantitative real-time PCR amplification was carried out using the Hieff UNICON® qPCR SYBR Green Master Mix (Yeasen Biotechnology, China).

Real-time Polymerase Chain Reaction:

Article Title: Dendrobine alleviates lung injury in septic mice by inhibiting mitochondrial-endoplasmic reticulum crosstalk-mediated NLRP3 inflammasome activation.
Article Snippet: EZ-press RNA purification kit (B0004D) was purchased from EZ Bioscience (California, USA).Lactic acid (LA) content assay kit (BC2235) was purchased from Solarbio (Beijing, China). .. Hifair® III 1st Strand cDNA Synthesis SuperMix (11120ES60), Hieff UNICON Universal Blue qPCR SYBR Green Master Mix (11184ES08), rProtein G agarose resin (36405ES08), and Rhod-2, AM (40776ES72) were purchased from Yeasen Biotechnology (Shanghai, China). .. Antibodies for NLRP3 (15101S), cleaved caspase-1 (4199S), HIF-1α (14179S), HK2 (2867S), and VDAC1 (4866S) were purchased from Cell Signaling Technology (Boston, MA, USA).

Article Title: A Heterogeneously structured Mg-Zn laminate alloy for gradient degradation behavior
Article Snippet: RNA was quantified by nanodrop (NanoDrop2000c, Thermo Scientific, USA). .. Complementary DNA (cDNA) was synthesized with Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN Biotechnology (Shanghai, China), and dNTPs using SuperScript III Reverse Transcriptase (Life Technologies, USA). .. Predesigned and validated primers were purchased from Sangon Biotech ( ).

Article Title: ACOT1-specific expression modulates metabolic reprogramming in diabetic cardiomyopathy: The role of SREBP1c lactylation in CD36-mediated lipotoxicity
Article Snippet: RNA concentration and purity were assessed spectrophotometrically (NanoDrop, Thermo Fisher Scientific, USA). .. For reverse transcription, 1 μg of total RNA was converted to complementary DNA (cDNA) using Hifair III First Strand cDNA Synthesis SuperMix (YEASEN, Shanghai, China).Quantitative real-time PCR (qPCR) was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad, California, USA) using SYBR Green Master Mix (YEASEN). ..

Article Title: Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli
Article Snippet: Total RNA was isolated from samples using the EASYspinPLus Bacterial RNA Rapid Extraction Kit (Aidlab, China). .. The cDNA was synthesized using Hifair® II 1st Strand cDNA Synthesis SuperMix for qPCR (Yeasen Biotechnology, China). .. Quantitative real-time PCR amplification was carried out using the Hieff UNICON® qPCR SYBR Green Master Mix (Yeasen Biotechnology, China).

SYBR Green Assay:

Article Title: Dendrobine alleviates lung injury in septic mice by inhibiting mitochondrial-endoplasmic reticulum crosstalk-mediated NLRP3 inflammasome activation.
Article Snippet: EZ-press RNA purification kit (B0004D) was purchased from EZ Bioscience (California, USA).Lactic acid (LA) content assay kit (BC2235) was purchased from Solarbio (Beijing, China). .. Hifair® III 1st Strand cDNA Synthesis SuperMix (11120ES60), Hieff UNICON Universal Blue qPCR SYBR Green Master Mix (11184ES08), rProtein G agarose resin (36405ES08), and Rhod-2, AM (40776ES72) were purchased from Yeasen Biotechnology (Shanghai, China). .. Antibodies for NLRP3 (15101S), cleaved caspase-1 (4199S), HIF-1α (14179S), HK2 (2867S), and VDAC1 (4866S) were purchased from Cell Signaling Technology (Boston, MA, USA).

Article Title: ACOT1-specific expression modulates metabolic reprogramming in diabetic cardiomyopathy: The role of SREBP1c lactylation in CD36-mediated lipotoxicity
Article Snippet: RNA concentration and purity were assessed spectrophotometrically (NanoDrop, Thermo Fisher Scientific, USA). .. For reverse transcription, 1 μg of total RNA was converted to complementary DNA (cDNA) using Hifair III First Strand cDNA Synthesis SuperMix (YEASEN, Shanghai, China).Quantitative real-time PCR (qPCR) was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad, California, USA) using SYBR Green Master Mix (YEASEN). ..

Synthesized:

Article Title: A Heterogeneously structured Mg-Zn laminate alloy for gradient degradation behavior
Article Snippet: RNA was quantified by nanodrop (NanoDrop2000c, Thermo Scientific, USA). .. Complementary DNA (cDNA) was synthesized with Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN Biotechnology (Shanghai, China), and dNTPs using SuperScript III Reverse Transcriptase (Life Technologies, USA). .. Predesigned and validated primers were purchased from Sangon Biotech ( ).

Article Title: Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli
Article Snippet: Total RNA was isolated from samples using the EASYspinPLus Bacterial RNA Rapid Extraction Kit (Aidlab, China). .. The cDNA was synthesized using Hifair® II 1st Strand cDNA Synthesis SuperMix for qPCR (Yeasen Biotechnology, China). .. Quantitative real-time PCR amplification was carried out using the Hieff UNICON® qPCR SYBR Green Master Mix (Yeasen Biotechnology, China).

other:

Article Title: ZNF33B Promotes Japanese Encephalitis Virus Infection by Regulating the Stability of M 6 A-Modified Trim25 to Control the Autophagy Process.
Article Snippet: The antibody against CSFV E2 was prepared in our lab. Alexa Fluor Plus 647 Goat anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody (Cat. # A32733), Alexa Fluor 488 goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody (Cat. # A32731), and Alexa Fluor 568 goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody (Cat. # A11011) were obtained from Invitrogen (USA).

Polymerase Chain Reaction:

Article Title: Identification of a novel spätzle in Macrobrachium rosenbergii and its regulatory effects on the immune responses against Vibrio parahaemolyticus and Vibrio alginolyticus.
Article Snippet: As a cytokine-like ligand, Spätzle activates the Toll signaling pathway through binding to Toll-like receptors (TLRs), thereby participating in the innate immune defense of crustaceans against diverse pathogens.. A novel Spätzle (MrSpz5) was cloned from Macrobrachium rosenbergii in this study.. The MrSpz5 cDNA is 1900 bp, comprising a 972-bp open reading frame (ORF) encoding 323 amino acids, a 662-bp 5′ UTR, and a 266-bp 3′ UTR.

Generated:

Article Title: Identification of a novel spätzle in Macrobrachium rosenbergii and its regulatory effects on the immune responses against Vibrio parahaemolyticus and Vibrio alginolyticus.
Article Snippet: As a cytokine-like ligand, Spätzle activates the Toll signaling pathway through binding to Toll-like receptors (TLRs), thereby participating in the innate immune defense of crustaceans against diverse pathogens.. A novel Spätzle (MrSpz5) was cloned from Macrobrachium rosenbergii in this study.. The MrSpz5 cDNA is 1900 bp, comprising a 972-bp open reading frame (ORF) encoding 323 amino acids, a 662-bp 5′ UTR, and a 266-bp 3′ UTR.



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