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TaKaRa smartscribe reverse transcriptase kit
Smartscribe Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase/SMARTScribe+Reverse+Transcriptase/pm42879275-252-162-166
Average 96 stars, based on 1142 article reviews

smartscribe reverse transcriptase kit - by Bioz Stars, 2026-10

96/100 stars

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Article Title: Antisense transcription reveals disease-associated adaptations in the human gut microbiome.
Article Snippet: The gut microbiome is a dynamic ecosystem in which microorganisms constantly adjust their transcriptional programmes.. Here we developed metastrand, a framework that integrates strand-aware metatranscriptomics and metagenomics to quantify mRNAs and antisense RNAs (asRNAs) in complex microbial communities at gene-level resolution.. In inflammatory bowel disease (IBD), microbial asRNA programmes converged across patients during active disease, correlated with faecal metabolites and calprotectin levels and remained stable during persistent inflammation, highlighting their potential as biomarkers of inflammatory activity in the gut.

Article Title: Genome architecture shapes transcriptional responses to topoisomerase depletion in C. elegans.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies H3K4me3 Active Motif Cat# 61979; RRID: AB_2793611 H3K27Ac Epicypher Cat# 13–0045; RRID: AB_2923489 H3K36me3 Abcam Cat#Ab9050; RRID: AB_306966 RNA Pol-II Millipore Cat# 05–952-I-100UG; RRID: AB_492629 H3K27me3 Diagenode Cat# C15410195; RRID: AB_2753161 Bacterial and virus strains E. coli OP50 Caenorhabditis Genetics Center OP50 Chemicals, peptides, and recombinant proteins indole-3-acetic-acid Thermo Fisher Scientific Cat# 87–51–4 TRIzolTM LS Reagent Thermo Fisher Scientific Cat# 10296028 Bio-11UTP Lumiprobe N/A DynabeadTM MyOneTM Streptavidin C1 Thermo Fisher Scientific Cat# 65001 HaltTM Protease Inhibitor Cocktail, EDTA-free (1003) Thermo Fisher Scientific Cat# 78425 T4 RNA Ligase 2, truncated KQ New England Biolabs Cat# M0373 Concanavalin A paramagnetic beads EpiCypher Cat# 21–1401 CutanaTM pAG-Tn5 EpiCypher Cat# 15–1117 Critical commercial assays SuperScript IV Reverse Transcriptase Fisher Cat# 18090050 High Sensitivity D1000 ScreenTape Agilent Cat# 5067–5584 High Sensitivity D1000 Reagents Agilent Cat# 5067–5585 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat# E7760S USB poly(A) Length Assay Kit Thermo Fisher Scientific Cat# 764551KT SMARTScribe Reverse Transcriptase kit Takara Bio Cat# 639537 QubitTM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 Deposited data Sequencing data This study GEO: , GEO: GSE309621, GEO: GSE309622 Experimental models: Organisms/strains C. elegans ERC83 ers55[top-2:degron:GFP] II; ieSi57 [eft-3p:TIR1:mRuby:unc-54 3′UTR + Cbrunc-119(+)] II Morao et al.25 top-2::degron::GFP C. elegans ERC84 ers56[top-1:degron:GFP] I; ieSi57 [eft-3p:TIR1:mRuby:unc-54 3′UTR + Cbrunc-119(+)] II Morao et al.25 top-1::degron::GFP C. elegans MHE403 ers56[top-1:degron:GFP] I; ers55[top-2:degron:GFP] II; ieSi57 [eft3p:TIR1:mRuby:unc-54 3′UTR + Cbr-unc-119(+)] II This study top-1::degron::GFP; top-2::degron::GFP C. elegans CA1200 ieSi57 [eft-3p:TIR1: mRuby:unc-54 3′UTR + Cbr-unc-119(+)] II Caenorhabditis Genetics Center CA1200 (Continued on next page) 16 Cell Reports 45, 118144, October 27, 2026 ..

Article Title: Discovery, Structural Characterization, and Preclinical Evaluation of Monoclonal Antibodies against Xylazine Poisoning
Article Snippet: Xylazine is a nonopioid sedative that has emerged as a major adulterant in the illicit drug supply, contributing to rising fatal overdoses across the United States.. Xylazine toxicity is not reversed by naloxone, and there are no current FDA-approved therapeutics to counteract its effects in humans.. To address this issue, we developed and characterized monoclonal antibodies (mAbs) capable of sequestering xylazine to prevent or treat acute toxicity.

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells
Article Snippet: .. Total RNA was reverse-transcribed into cDNA using template switch oligonucleotide (TSO), primers for human TCR constant regions and SMARTScribe reverse transcriptases (Clontech) according to the manufacturer’s instructions. ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: The circadian isoform landscape of mouse livers
Article Snippet: .. First strand reverse transcription was performed for 1 h at 42°C, before being heat inactivated for 5 min at 70°C, using the Smartscribe Reverse Transcriptase (Clontech). ..

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Article Title: Leukemic stem cell subtypes determine venetoclax resistance and therapeutic vulnerabilities in AML.
Article Snippet: RNA quality assessment and quantification were performed with Bioanalyzer using Agilent RNA 6000 Pico Kit (Agilent, cat #5067-1513). .. Whole-transcriptome amplification was performed using a modified Smartseq2 protocol, with 5 μL of a modified RT Buffer containing 1 × SMART First Strand Buffer (Takara Bio Clontech, cat #639538), 1 mmol/L dithiothreitol (Takara Bio Clontech), 1 μmol/L template switching oligo (IDT), 10 U/μL SMARTScribe (Takara Bio Clontech, cat #639538), and 1 U/μL RNasin Plus RNase Inhibitor (Promega, cat #N2615). .. Tagmentation of cDNA was done using Nextera XT DNA Library Preparation Kit (Illumina, cat #FC-121-1030).

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Article Title: Divergent roles of TRPV1 and neuropeptides in mouse model of atopic dermatitis.
Article Snippet: TO THE EDITOR Atopic dermatitis (AD) is a chronic itchy and inflammatory skin disease.. Despite these symptoms, which cause significant distress in patients, the neuroprocessing of itch and inflammation in AD is poorly understood.. Loss of brain natriuretic peptide (BNP) significantly decreases itch and inflammation in MC9093-induced AD (Wheeler et al, 2024); however, the roles of other neuropeptides in AD remain unclear.

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