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rt reaction  (highQu Inc)


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    Structured Review

    highQu Inc rt reaction
    Rt Reaction, supplied by highQu Inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase/HighScriber+Reverse+Transcriptase+Mix/pmc11317168-102-1-37
    Average 93 stars, based on 6 article reviews
    rt reaction - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (K Ca 3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Article Title: Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse transcribed using the cDNA Synthesis Kit (Promega, Madison, WI, USA). .. The RT reaction was performed at 37°C for 15 min, followed by inactivation of reverse transcriptase at 85°C for 15 s using a thermal cycler. qPCR experiments were conducted using ORATM qPCR Green ROX L Mix (2×) (highQu, Kraichtal, Germany) with specific qPCR primers for target RNA or 16S rRNA. .. A Stratagene Mx3000P thermal cycler was used with specified conditions.

    Article Title: One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
    Article Snippet: The supernatant was further diluted 2 μL in 198 μL extraction buffer General (10 X diluted to 1 X, Bioreba AG, Switzerland) and 2 μL of this dilution were used for the DiRT. .. The mastermix for the multiplex DiRT-PCRs were done using the same primers, probes, fluorophore-combinations as described in Prinz et al. (2022) using an optimized concentration protocol version with HighScriber reverse transcriptase and HighScriber Buffer (HighQu, Germany, for details, see Supp3). ..

    Polymerase Chain Reaction:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (K Ca 3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (K Ca 3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Real-time Polymerase Chain Reaction:

    Article Title: Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse transcribed using the cDNA Synthesis Kit (Promega, Madison, WI, USA). .. The RT reaction was performed at 37°C for 15 min, followed by inactivation of reverse transcriptase at 85°C for 15 s using a thermal cycler. qPCR experiments were conducted using ORATM qPCR Green ROX L Mix (2×) (highQu, Kraichtal, Germany) with specific qPCR primers for target RNA or 16S rRNA. .. A Stratagene Mx3000P thermal cycler was used with specified conditions.

    Multiplex Assay:

    Article Title: One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
    Article Snippet: The supernatant was further diluted 2 μL in 198 μL extraction buffer General (10 X diluted to 1 X, Bioreba AG, Switzerland) and 2 μL of this dilution were used for the DiRT. .. The mastermix for the multiplex DiRT-PCRs were done using the same primers, probes, fluorophore-combinations as described in Prinz et al. (2022) using an optimized concentration protocol version with HighScriber reverse transcriptase and HighScriber Buffer (HighQu, Germany, for details, see Supp3). ..

    Concentration Assay:

    Article Title: One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
    Article Snippet: The supernatant was further diluted 2 μL in 198 μL extraction buffer General (10 X diluted to 1 X, Bioreba AG, Switzerland) and 2 μL of this dilution were used for the DiRT. .. The mastermix for the multiplex DiRT-PCRs were done using the same primers, probes, fluorophore-combinations as described in Prinz et al. (2022) using an optimized concentration protocol version with HighScriber reverse transcriptase and HighScriber Buffer (HighQu, Germany, for details, see Supp3). ..

    other:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model.
    Article Snippet: Measuring released TGF-β1 in cell culture supernatants was performed by NS using Mouse TGF-β 1 DuoSet ELISA, DuoSet ELISA Ancillary Reagent Kit 1 and Sample Activation Kit 1 (all R&D Systems, DY1679-05, DY007B, DY010) according to the manufacturer’s instruction.



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