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protoscript ii reverse transcriptase  (New England Biolabs)


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    Structured Review

    New England Biolabs protoscript ii reverse transcriptase
    Protoscript Ii Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm42901825-52-6-10
    Average 99 stars, based on 1861 article reviews
    protoscript ii reverse transcriptase - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Screening for Pea Seed-borne Mosaic Virus Resistance (PSbMV) in Lentil and Development of Associated KASP Markers
    Article Snippet: .. Reverse transcription was performed using the First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with oligo(dT)23 VN primers following the manufacturer’s quick protocol ( www.neb.com/E6560 ). ..

    Article Title: Loss of protein function resulting in severe phenotypes of X-linked myotubular myopathy caused by novel synonymous and splice-site variants in the MTM1 gene.
    Article Snippet: .. Complementary DNA (cDNA) was synthesized using ProtoScript II Reverse Transcriptase (NEB, E6560) with oligo-dT primers. ..

    Article Title: Four Years of Continuous Weekly Wastewater Analysis Reveals Sequencing as a Robust Alternative to PCR for Respiratory Virome Surveillance
    Article Snippet: After the completion of the bead beating step, samples were again centrifuged at 14-17 x 1,000 RPM for 2 min. Post-centrifugation, DNA and RNA elements were extracted using the Qiagen QIAamp VIRAL RNA Mini Kit. .. Reverse transcription of RNA to cDNA was carried out with Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), NEBNext Ultra II Non-Directional RNA Second Strand Module (New England Biolabs Inc.), and Random Primer 6 (New England Biolabs Inc.) before mixing it with DNA for library construction. .. Libraries were constructed with Twist Library Preparation EF 2.0 Kit and Twist Universal Adaptor System (TWIST Biosciences) using 25 ng of cDNA and DNA mix.

    Article Title: Endothelial CXCL12 is dispensable for adipose tissue function
    Article Snippet: .. Total RNA was isolated with innuSOLV RNA reagent (Analytik Jena, 845-SB-2090100), and reverse-transcribed using cDNA synthesis kit (ProtoScript II First Strand cDNA Synthesis Kit, NEB). .. Quantitative PCR was performed using the cDNA as template with Luna Universal qPCR Master Mix (NEB).

    cDNA Synthesis:

    Article Title: Screening for Pea Seed-borne Mosaic Virus Resistance (PSbMV) in Lentil and Development of Associated KASP Markers
    Article Snippet: .. Reverse transcription was performed using the First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with oligo(dT)23 VN primers following the manufacturer’s quick protocol ( www.neb.com/E6560 ). ..

    Article Title: Comparison of Human Costal-Derived Chondrocyte Donor Sites and Microtia-Derived Chondrocytes for Auricular Tissue Engineering.
    Article Snippet: RNA was quantified using the NanoDrop spectrophotometer (Thermo Scientific, USA), and only samples with an A260/A280 greater than 1.8 were used. .. cDNA was prepared from 1 μg of RNA via the ProScript II First Strand cDNA Synthesis Kit (New England Biolabs, USA) according to the manufacturer’s instructions. .. Real-time quantitative PCR was performed using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, USA) on the CFX Duet Real-Time PCR System (Bio-Rad, USA).

    Article Title: Exposure and Bioaccumulation of Mercury Impacted the Extent of Cellular Damage and Oxidative Stress in Local Chickens Around Ilesha Gold Mining Areas of Nigeria.
    Article Snippet: Mercury (Hg) is a wellknown global pollutant of environmental concern.. Uncontrolled mining activities represent an avenue for its release and possible environmental contamination.. Ilesha gold mining areas are characterized by extensive artisan and smallscale gold mining (ASGM) activities.

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: Four Years of Continuous Weekly Wastewater Analysis Reveals Sequencing as a Robust Alternative to PCR for Respiratory Virome Surveillance
    Article Snippet: After the completion of the bead beating step, samples were again centrifuged at 14-17 x 1,000 RPM for 2 min. Post-centrifugation, DNA and RNA elements were extracted using the Qiagen QIAamp VIRAL RNA Mini Kit. .. Reverse transcription of RNA to cDNA was carried out with Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), NEBNext Ultra II Non-Directional RNA Second Strand Module (New England Biolabs Inc.), and Random Primer 6 (New England Biolabs Inc.) before mixing it with DNA for library construction. .. Libraries were constructed with Twist Library Preparation EF 2.0 Kit and Twist Universal Adaptor System (TWIST Biosciences) using 25 ng of cDNA and DNA mix.

    Article Title: Native pathogen-phage networks reveal multilayered defense and gut-active anti-phage immunity.
    Article Snippet: .. For RNA-based qRT-PCR, cDNA was generated from purified RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). ..

    Article Title: Endothelial CXCL12 is dispensable for adipose tissue function
    Article Snippet: .. Total RNA was isolated with innuSOLV RNA reagent (Analytik Jena, 845-SB-2090100), and reverse-transcribed using cDNA synthesis kit (ProtoScript II First Strand cDNA Synthesis Kit, NEB). .. Quantitative PCR was performed using the cDNA as template with Luna Universal qPCR Master Mix (NEB).

    Synthesized:

    Article Title: Loss of protein function resulting in severe phenotypes of X-linked myotubular myopathy caused by novel synonymous and splice-site variants in the MTM1 gene.
    Article Snippet: .. Complementary DNA (cDNA) was synthesized using ProtoScript II Reverse Transcriptase (NEB, E6560) with oligo-dT primers. ..

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Generated:

    Article Title: Native pathogen-phage networks reveal multilayered defense and gut-active anti-phage immunity.
    Article Snippet: .. For RNA-based qRT-PCR, cDNA was generated from purified RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). ..

    Purification:

    Article Title: Native pathogen-phage networks reveal multilayered defense and gut-active anti-phage immunity.
    Article Snippet: .. For RNA-based qRT-PCR, cDNA was generated from purified RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). ..

    Isolation:

    Article Title: Endothelial CXCL12 is dispensable for adipose tissue function
    Article Snippet: .. Total RNA was isolated with innuSOLV RNA reagent (Analytik Jena, 845-SB-2090100), and reverse-transcribed using cDNA synthesis kit (ProtoScript II First Strand cDNA Synthesis Kit, NEB). .. Quantitative PCR was performed using the cDNA as template with Luna Universal qPCR Master Mix (NEB).



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