gene exp rpii140 dm02134593 g1 (Thermo Fisher)
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gene exp rpii140 dm02134593 g1
Gene Exp Rpii140 Dm02134593 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+sequence+(refseq)+and+annotations/Gene+Exp%2E+RpII140%2C+Dm02134593_g1/pmc06934411-537-5--1
Average 90 stars, based on 1 article reviews
Gene Exp Rpii140 Dm02134593 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+sequence+(refseq)+and+annotations/Gene+Exp%2E+RpII140%2C+Dm02134593_g1/pmc06934411-537-5--1
Average 90 stars, based on 1 article reviews
gene exp rpii140 dm02134593 g1 - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis"
Article Title: Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis
Journal: Current biology : CB
doi: 10.1016/j.cub.2018.03.068
Figure Legend Snippet: Key Resources Table
Techniques Used: Recombinant, Electron Microscopy, Reverse Transcription, Plasmid Preparation, Software, Microscopy
Related Articles
Real-time Polymerase Chain Reaction:Article Title: Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry Article Snippet: Reverse transcription was performed with the iScript kit (Biorad, 1708841) according to the manufacturer’s protocol. .. Real-time PCR was done on a CFX96 QPCR detection system (Bio-RAD) using TaqMan Fast Advanced Master Mix with the following TaqMan probes (Applied Biosystems, Life Technologies), following classical TaqMan probes protocol: Toll-2=18W= Dm01841837_s1 (position 5228 / GenBank NM_057466 , amplicon = 152bp) Toll-6= Dm01822826_s1 (position 5259 / GenBank NM_001259836 , amplicon = 109bp) RPl32: House-keeping gene reference: Dm02151827_g1: (exon 2-3 boundary, 377 / GenBank NM_001144655 , amplicon = 72bp) RPII140: House-keeping gene: Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia. Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270, the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudioTM 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270 , the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules Article Snippet: For quantifying the mRNA knockdown of these shRNAs, total RNA was extracted from oocytes using TRIzol Reagent (Life Technologies) and reverse transcribed into cDNA using the High-Capacity cDNA Reverse-Transcription Kit (Applied Biosystems). .. Quantitative PCR was performed on a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies); Dm03420510_g1 for Haspin , Dm02141491_g1 for Deterin , Dm01804657_g1 for Bub1 , Dm0103608_g1 for Su(var)205 , and Amplification:Article Title: Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry Article Snippet: Reverse transcription was performed with the iScript kit (Biorad, 1708841) according to the manufacturer’s protocol. .. Real-time PCR was done on a CFX96 QPCR detection system (Bio-RAD) using TaqMan Fast Advanced Master Mix with the following TaqMan probes (Applied Biosystems, Life Technologies), following classical TaqMan probes protocol: Toll-2=18W= Dm01841837_s1 (position 5228 / GenBank NM_057466 , amplicon = 152bp) Toll-6= Dm01822826_s1 (position 5259 / GenBank NM_001259836 , amplicon = 109bp) RPl32: House-keeping gene reference: Dm02151827_g1: (exon 2-3 boundary, 377 / GenBank NM_001144655 , amplicon = 72bp) RPII140: House-keeping gene: Quantitative RT-PCR:Article Title: Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry Article Snippet: Reverse transcription was performed with the iScript kit (Biorad, 1708841) according to the manufacturer’s protocol. .. Real-time PCR was done on a CFX96 QPCR detection system (Bio-RAD) using TaqMan Fast Advanced Master Mix with the following TaqMan probes (Applied Biosystems, Life Technologies), following classical TaqMan probes protocol: Toll-2=18W= Dm01841837_s1 (position 5228 / GenBank NM_057466 , amplicon = 152bp) Toll-6= Dm01822826_s1 (position 5259 / GenBank NM_001259836 , amplicon = 109bp) RPl32: House-keeping gene reference: Dm02151827_g1: (exon 2-3 boundary, 377 / GenBank NM_001144655 , amplicon = 72bp) RPII140: House-keeping gene: Expressing:Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia. Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270, the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudioTM 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Apoptosis and motor deficits in SPG76 hereditary spastic paraplegia: Calpain 2 inhibition as therapeutic strategy. Article Snippet: .. The expression levels of CalpB in the CalpB -/- Drosophila model were analysed by using CalpB (Dm01822543_g1) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes. Article Snippet: For the analysis of CG13531 gene levels in the SPG11 Drosophila CRISPR line total RNA was isolated from 10 larvae with Trizol reagent and purified using Direct-Zol TM RNA MiniPrep kit (Zymo Research) according to the manufacturer’s instructions. .. The expression levels of CG13531 were analysed by using a specific gene expression assay in the exons 3–4 (Dm01830473_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270 , the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Gene Expression:Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia. Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270, the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudioTM 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Apoptosis and motor deficits in SPG76 hereditary spastic paraplegia: Calpain 2 inhibition as therapeutic strategy. Article Snippet: .. The expression levels of CalpB in the CalpB -/- Drosophila model were analysed by using CalpB (Dm01822543_g1) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes. Article Snippet: For the analysis of CG13531 gene levels in the SPG11 Drosophila CRISPR line total RNA was isolated from 10 larvae with Trizol reagent and purified using Direct-Zol TM RNA MiniPrep kit (Zymo Research) according to the manufacturer’s instructions. .. The expression levels of CG13531 were analysed by using a specific gene expression assay in the exons 3–4 (Dm01830473_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270 , the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Article Title: Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules Article Snippet: For quantifying the mRNA knockdown of these shRNAs, total RNA was extracted from oocytes using TRIzol Reagent (Life Technologies) and reverse transcribed into cDNA using the High-Capacity cDNA Reverse-Transcription Kit (Applied Biosystems). .. Quantitative PCR was performed on a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies); Dm03420510_g1 for Haspin , Dm02141491_g1 for Deterin , Dm01804657_g1 for Bub1 , Dm0103608_g1 for Su(var)205 , and other:Article Title: Molecular competition can shape enhancer activity in the Drosophila embryo Article Snippet: Article Title: Molecular competition can shape enhancer activity in the Drosophila embryo Article Snippet: The TaqMan FAM probes used for each gene were DM01803576_g1 for Piezo , Dm01825813_g1 for Mcr , Dm01803642_g1 for Btk29A , and DM02134593_g1 for RpII140 . Drosophila:Article Title: Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia Article Snippet: Then, 1 μg/sample was reverse-transcribed into cDNA using the Superscript First Strand Synthesis System for RT–PCR kit (Thermo Fisher Scientific) and random hexamers. .. The expression levels of CG5270 , the Drosophila orthologue of the human ZFYVE26 gene, were analysed by quantitative real-time PCR on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) by using a CG5270 specific gene expression assay (Dm02139099_g1, Applied Biosystems, Thermo Fisher Scientific) and RNA polymerase II 140 kD subunit (RpII140, Control:Article Title: Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules Article Snippet: For quantifying the mRNA knockdown of these shRNAs, total RNA was extracted from oocytes using TRIzol Reagent (Life Technologies) and reverse transcribed into cDNA using the High-Capacity cDNA Reverse-Transcription Kit (Applied Biosystems). .. Quantitative PCR was performed on a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies); Dm03420510_g1 for Haspin , Dm02141491_g1 for Deterin , Dm01804657_g1 for Bub1 , Dm0103608_g1 for Su(var)205 , and |