nanopore rapid barcoding sequencing kit (Oxford Nanopore)

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Oxford Nanopore nanopore rapid barcoding sequencing kit
Nanopore Rapid Barcoding Sequencing Kit, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+barcoding+kit/barcoding+kit/pm40653462-62-12-16
Average 90 stars, based on 1 article reviews

nanopore rapid barcoding sequencing kit - by Bioz Stars, 2026-09

90/100 stars

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Polymerase Chain Reaction:

Article Title: Influenza A(H5N1) Virus Clade 2.3.2.1a in Traveler Returning to Australia from India, 2024
Article Snippet: .. We used PCR amplicons for Nanopore library preparation with rapid ONT barcoding kit (Oxford Nanopore Technologies, https://nanoporetech.com ), loaded them into a standard flow cell, and sequenced them (MinION Mk1b for 8 hours). ..

Sequencing:

Article Title: Atypical Actinobacillus pleuropneumoniae serotype 12 strains with a higher virulence potential.
Article Snippet: For the Illumina MiniSeq, samples were diluted to 0.20 ng/μL. .. Sequencing of isolates on the Nanopore was performed using the Oxford Nanopore Rapid Barcoding Sequencing Kit (Oxford Nanopore Technologies, Oxford, UK) and GridION. ..

Article Title: Fungal Biodeterioration Risk in Monastic Libraries without Climate Control
Article Snippet: .. The Nanopore sequencing platform (barcoding- and library preparation kits, sequencing devices and software were all from Oxford Nanopore Technologies, Oxford UK) was selected for this metagenomic study. .. The PCR Barcoding Kit (SQK-PBK004) was used as described by Tichy et al. [ ] but adapted for fungi: customized tailed primers ITS1(F) and ITS4(R) (New England Biolabs, Ipswich, MA, USA) from a stock solution of 2.5 pmol/μL were used for the Barcoding PCR reaction with a corresponding annealing temperature of 55 °C and an extension temperature of 65 °C for 45 s. Barcodes 01–04 were assigned to the four historic libraries, followed by pooling of all four samples for the preparation of a single sequencing library.

Article Title: Universal whole-genome Oxford nanopore sequencing of SARS-CoV-2 using tiled amplicons.
Article Snippet: .. The Oxford Nanopore Rapid Barcoding library kit generates barcoded sequencing libraries from pooled amplicons, the transposase approach simultaneously cleaves amplicons and attaches barcoded tags to the cleaved ends. .. These amplicons are then subjected to Oxford Nanopore library preparation, using methods that directly add adapters to the amplicons.

Article Title: Draft genome sequence of Ruoffia tabacinasalis isolated from a bovine nasal swab: a novel member of the bovine nasal microbiota.
Article Snippet: Three loops of overnight culture (originating from a single colony) were suspended in phosphate-buffered saline and vortexed in a bead beater at 3,500 rpm for 2 minutes for genomic DNA isolation using the Qiagen QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Next, the Rapid Barcoding Library Preparation Kit and AMPure XP beads were used to prepare the sequencing library (Oxford Nanopore Technologies). .. Sequencing was performed using the Oxford Nanopore MinION Mk1C sequencer with the flow cell R9.4.1 (FLO-MIN106D) for 30 hours.

Nanopore Sequencing:

Article Title: Phylogenomics, phenotypic, and functional traits of five novel (Earth-derived) bacterial species isolated from the International Space Station and their prevalence in metagenomes
Article Snippet: Additionally, Sporosarcina thermotolerans CCUG 53480 T , kindly provided by Dr. Edward Moore from the Culture Collection University of Gothenburg (CCUG), Sweden, also underwent WGS with the use of Nanopore MinION (Oxford Nanopore Technologies, Oxford, UK). .. Nanopore sequencing libraries were prepared using the SQK-RBK114.24 rapid chemistry-based barcoding kit (Oxford Nanopore Technologies, Oxford, UK). ..

Software:

Multiplex Assay:

Article Title: Harnessing non-standard nucleic acids for highly sensitive icosaplex (20-plex) detection of microbial threats
Article Snippet: .. To multiplex samples, we then created 10 unique barcoding primers that contained a 24-nt barcode region using sequences from an Oxford Nanopore Technologies barcoding kit. ..

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