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G Biosciences 5% blot-quickblocker
5% Blot Quickblocker, supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblock+blocking+solution/blot+quickblocker/us11459557-1556-10-11
Average 90 stars, based on 1 article reviews
5% blot-quickblocker - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Membrane:

Article Title: RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication.
Article Snippet: The membrane was blocked in 5% BLOT-QuickBlocker (G-Bioscience no. 786-011) in TBS-T (20 mM Tris– HCl, 150 mM NaCl and 0.1% (w/v) Tween 20) at room temperature (RT) for 1 h and incubated with primary antibodies at 4°C overnight.

Article Title: Noncanonical Activity of Med4 as a Gatekeeper of Metastasis through Epigenetic Control of Integrin Signaling
Article Snippet: Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG-HRP (Cell Signaling Technology 7076S, 1:5000), anti-goat IgG-HRP (Santa Cruz Biotechnology sc-2354, 1:5000)] in 2.5% BLOT-QuickBlocker (G Biosciences 786-011) in TBST for 1Lh at room temperature.

Article Title: RNF4 prevents genomic instability caused by chronic DNA under-replication.
Article Snippet: Primary antibodies were incubated in 5% BLOT-QuickBlocker (GBiosciences 786–011) as follows: rabbit anti-MCM10 (Bethyl A300–131A, RRID:AB_2142119; 1:500), mouse anti-α-Tubulin (Sigma T9026, RRID:AB_477593, clone DM1A; 1:5000), goat anti-RNF4 (R&D Systems AF7964; 1:500), rabbit anti-SUMO2/3 (Millipore 07–2167, M.K.

Article Title: RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication
Article Snippet: The membrane was blocked in 5% BLOT-QuickBlocker (G-Bioscience no. 786-011) in TBS-T (20 mM Tris–HCl, 150 mM NaCl and 0.1% (w/v) Tween 20) at room temperature (RT) for 1 h and incubated with primary antibodies at 4°C overnight.

Article Title: A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells
Article Snippet: Primary antibodies were diluted in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows: rabbit anti-Mcm10 (Novus H00055388-D01P, RRID:AB_11047378; 1:500) mouse anti-GAPDH (GeneTex GTX627408, RRID:AB_11174761; 1:5000), mouse anti-Tubulin (Sigma T9026, clone DM1A, RRID:AB_477593; 1:10 000), mouse anti-PCNA (Abcam ab29, RRID:AB_303394; 1:3000), rabbit anti-Lamin B1 (Proteintech 12987-1-AP, RRID:AB_2136290; 1:3000), rabbit anti-ATM (Proteintech 27156-1-AP, RRID:AB_2880780; 1:1000), mouse anti-KAP1 (Proteintech 66630-1-IG, RRID:AB_2732886; 1:5000), rabbit anti-phosphorylated KAP1 (Abcam ab133440; 1:1000), rabbit anti-H2AX (Bethyl A300-083A, RRID:AB_203289; 1:10 000), rabbit anti-γ-H2AX (Bethyl A300-081A, RRID:AB_203288; 1:5000).

Article Title: Reversible Assembly of Proteolysis Targeting Chimeras.
Article Snippet: PROteolysis TArgeting Chimeras (PROTACs) are of significant current interest for the development of probe molecules and drug leads.. However, they suffer from certain limitations.. PROTACs are rule-breaking molecules with sub-optimal cellular permeability, solubility, and other drug-like properties.

Article Title: Caffeic acid phenethyl ester mediates apoptosis in serum-starved HT29 colon cancer cells through modulation of heat shock proteins and MAPK pathways.
Article Snippet: Funding information Rashtriya Uchchatar Shiksha Abhiyan RUSA 2.0, University of Madras, and Govt. of India Abstract Colorectal cancer (CRC) is among the most prevalent gastrointestinal cancers of epithelial origin worldwide, with over 2 million cases detected every year.. Emerging evidence suggests a significant increase in the levels of inflammatory and stress‐ related markers in patients with CRC, indicating that oxidative stress and lipid peroxidation may influence signalling cascades involved in the progression of the disease.. However, the precise molecular and cellular basis underlying CRC and their modulations during bioactive compound exposure have not yet been deciphered.

Article Title: FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination.
Article Snippet: Primary antibodies were incubated in 5% 18 Cell Reports 42, 113523, December 26, 2023 BLOT-QuickBlocker (G-Biosciences 786-011) as follows: mouse anti-PCNA (Abcam, ab29; 1:3000), rabbit anti-Ubiquityl-PCNA (Lys164) (Cell Signaling, D5C7P, 13439; 1:1000), rabbit anti-RPA32 (S33) (Bethyl, A300-246A; 1:2000), rabbit anti-gH2AX (Bethyl, A300-081A; 1:2000), rabbit anti-H2AX (Bethyl, A300-082A; 1:5000), rabbit anti-p-p53 (S15) (Cell Signaling, 9284S; 1:500), mouse anti-p53 (Santa Cruz, sc-126; 1:2000), rabbit anti-p21 (Santa Cruz, sc-397 clone C19; 1:1000), rabbit anti-FANCD2 (Abcam, ab108928; 1:2000), mouse anti-FANCL (Proteintech, 66639-1-Ig; 1:1,000), rabbit anti-RAD18 (Bethyl, A300-340A; 1:1000), rabbit anti-MCM2 (Cell Signaling, 4007S; 1:1000; BD Biosciences, 610701; 1:1000; Proteintech, 10513-1-AP; 1:1000), mouse anti-GAPDH (GeneTex, GTX627408; 1:10000), mouse anti-Ku86 (Santa Cruz, B-1, sc-5280; 1:500), mouse anti-a-tubulin (Millipore, T9026, clone DM1A; 1:10000), rabbit anti-ORC2 (Thermo Fisher, PA5-110063, 1:1000), rabbit anti-Histone H4 (Abcam, ab10158, 1:200,000).

Incubation:

Article Title: RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication.
Article Snippet: The membrane was blocked in 5% BLOT-QuickBlocker (G-Bioscience no. 786-011) in TBS-T (20 mM Tris– HCl, 150 mM NaCl and 0.1% (w/v) Tween 20) at room temperature (RT) for 1 h and incubated with primary antibodies at 4°C overnight.

Article Title: Noncanonical Activity of Med4 as a Gatekeeper of Metastasis through Epigenetic Control of Integrin Signaling
Article Snippet: Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG-HRP (Cell Signaling Technology 7076S, 1:5000), anti-goat IgG-HRP (Santa Cruz Biotechnology sc-2354, 1:5000)] in 2.5% BLOT-QuickBlocker (G Biosciences 786-011) in TBST for 1Lh at room temperature.

Article Title: RNF4 prevents genomic instability caused by chronic DNA under-replication.
Article Snippet: Primary antibodies were incubated in 5% BLOT-QuickBlocker (GBiosciences 786–011) as follows: rabbit anti-MCM10 (Bethyl A300–131A, RRID:AB_2142119; 1:500), mouse anti-α-Tubulin (Sigma T9026, RRID:AB_477593, clone DM1A; 1:5000), goat anti-RNF4 (R&D Systems AF7964; 1:500), rabbit anti-SUMO2/3 (Millipore 07–2167, M.K.

Article Title: RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication
Article Snippet: The membrane was blocked in 5% BLOT-QuickBlocker (G-Bioscience no. 786-011) in TBS-T (20 mM Tris–HCl, 150 mM NaCl and 0.1% (w/v) Tween 20) at room temperature (RT) for 1 h and incubated with primary antibodies at 4°C overnight.

Article Title: A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells
Article Snippet: Primary antibodies were diluted in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows: rabbit anti-Mcm10 (Novus H00055388-D01P, RRID:AB_11047378; 1:500) mouse anti-GAPDH (GeneTex GTX627408, RRID:AB_11174761; 1:5000), mouse anti-Tubulin (Sigma T9026, clone DM1A, RRID:AB_477593; 1:10 000), mouse anti-PCNA (Abcam ab29, RRID:AB_303394; 1:3000), rabbit anti-Lamin B1 (Proteintech 12987-1-AP, RRID:AB_2136290; 1:3000), rabbit anti-ATM (Proteintech 27156-1-AP, RRID:AB_2880780; 1:1000), mouse anti-KAP1 (Proteintech 66630-1-IG, RRID:AB_2732886; 1:5000), rabbit anti-phosphorylated KAP1 (Abcam ab133440; 1:1000), rabbit anti-H2AX (Bethyl A300-083A, RRID:AB_203289; 1:10 000), rabbit anti-γ-H2AX (Bethyl A300-081A, RRID:AB_203288; 1:5000).

Article Title: Reversible Assembly of Proteolysis Targeting Chimeras.
Article Snippet: PROteolysis TArgeting Chimeras (PROTACs) are of significant current interest for the development of probe molecules and drug leads.. However, they suffer from certain limitations.. PROTACs are rule-breaking molecules with sub-optimal cellular permeability, solubility, and other drug-like properties.

Article Title: Caffeic acid phenethyl ester mediates apoptosis in serum-starved HT29 colon cancer cells through modulation of heat shock proteins and MAPK pathways.
Article Snippet: Funding information Rashtriya Uchchatar Shiksha Abhiyan RUSA 2.0, University of Madras, and Govt. of India Abstract Colorectal cancer (CRC) is among the most prevalent gastrointestinal cancers of epithelial origin worldwide, with over 2 million cases detected every year.. Emerging evidence suggests a significant increase in the levels of inflammatory and stress‐ related markers in patients with CRC, indicating that oxidative stress and lipid peroxidation may influence signalling cascades involved in the progression of the disease.. However, the precise molecular and cellular basis underlying CRC and their modulations during bioactive compound exposure have not yet been deciphered.

Article Title: FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination.
Article Snippet: Primary antibodies were incubated in 5% 18 Cell Reports 42, 113523, December 26, 2023 BLOT-QuickBlocker (G-Biosciences 786-011) as follows: mouse anti-PCNA (Abcam, ab29; 1:3000), rabbit anti-Ubiquityl-PCNA (Lys164) (Cell Signaling, D5C7P, 13439; 1:1000), rabbit anti-RPA32 (S33) (Bethyl, A300-246A; 1:2000), rabbit anti-gH2AX (Bethyl, A300-081A; 1:2000), rabbit anti-H2AX (Bethyl, A300-082A; 1:5000), rabbit anti-p-p53 (S15) (Cell Signaling, 9284S; 1:500), mouse anti-p53 (Santa Cruz, sc-126; 1:2000), rabbit anti-p21 (Santa Cruz, sc-397 clone C19; 1:1000), rabbit anti-FANCD2 (Abcam, ab108928; 1:2000), mouse anti-FANCL (Proteintech, 66639-1-Ig; 1:1,000), rabbit anti-RAD18 (Bethyl, A300-340A; 1:1000), rabbit anti-MCM2 (Cell Signaling, 4007S; 1:1000; BD Biosciences, 610701; 1:1000; Proteintech, 10513-1-AP; 1:1000), mouse anti-GAPDH (GeneTex, GTX627408; 1:10000), mouse anti-Ku86 (Santa Cruz, B-1, sc-5280; 1:500), mouse anti-a-tubulin (Millipore, T9026, clone DM1A; 1:10000), rabbit anti-ORC2 (Thermo Fisher, PA5-110063, 1:1000), rabbit anti-Histone H4 (Abcam, ab10158, 1:200,000).

Binding Assay:

Article Title: RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication.
Article Snippet: The membrane was blocked in 5% BLOT-QuickBlocker (G-Bioscience no. 786-011) in TBS-T (20 mM Tris– HCl, 150 mM NaCl and 0.1% (w/v) Tween 20) at room temperature (RT) for 1 h and incubated with primary antibodies at 4°C overnight.

Article Title: Noncanonical Activity of Med4 as a Gatekeeper of Metastasis through Epigenetic Control of Integrin Signaling
Article Snippet: Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG-HRP (Cell Signaling Technology 7076S, 1:5000), anti-goat IgG-HRP (Santa Cruz Biotechnology sc-2354, 1:5000)] in 2.5% BLOT-QuickBlocker (G Biosciences 786-011) in TBST for 1Lh at room temperature.

Article Title: RNF4 prevents genomic instability caused by chronic DNA under-replication.
Article Snippet: Primary antibodies were incubated in 5% BLOT-QuickBlocker (GBiosciences 786–011) as follows: rabbit anti-MCM10 (Bethyl A300–131A, RRID:AB_2142119; 1:500), mouse anti-α-Tubulin (Sigma T9026, RRID:AB_477593, clone DM1A; 1:5000), goat anti-RNF4 (R&D Systems AF7964; 1:500), rabbit anti-SUMO2/3 (Millipore 07–2167, M.K.

Article Title: RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication
Article Snippet: The membrane was blocked in 5% BLOT-QuickBlocker (G-Bioscience no. 786-011) in TBS-T (20 mM Tris–HCl, 150 mM NaCl and 0.1% (w/v) Tween 20) at room temperature (RT) for 1 h and incubated with primary antibodies at 4°C overnight.

Article Title: A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells
Article Snippet: Primary antibodies were diluted in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows: rabbit anti-Mcm10 (Novus H00055388-D01P, RRID:AB_11047378; 1:500) mouse anti-GAPDH (GeneTex GTX627408, RRID:AB_11174761; 1:5000), mouse anti-Tubulin (Sigma T9026, clone DM1A, RRID:AB_477593; 1:10 000), mouse anti-PCNA (Abcam ab29, RRID:AB_303394; 1:3000), rabbit anti-Lamin B1 (Proteintech 12987-1-AP, RRID:AB_2136290; 1:3000), rabbit anti-ATM (Proteintech 27156-1-AP, RRID:AB_2880780; 1:1000), mouse anti-KAP1 (Proteintech 66630-1-IG, RRID:AB_2732886; 1:5000), rabbit anti-phosphorylated KAP1 (Abcam ab133440; 1:1000), rabbit anti-H2AX (Bethyl A300-083A, RRID:AB_203289; 1:10 000), rabbit anti-γ-H2AX (Bethyl A300-081A, RRID:AB_203288; 1:5000).

Article Title: Reversible Assembly of Proteolysis Targeting Chimeras.
Article Snippet: PROteolysis TArgeting Chimeras (PROTACs) are of significant current interest for the development of probe molecules and drug leads.. However, they suffer from certain limitations.. PROTACs are rule-breaking molecules with sub-optimal cellular permeability, solubility, and other drug-like properties.

Article Title: Caffeic acid phenethyl ester mediates apoptosis in serum-starved HT29 colon cancer cells through modulation of heat shock proteins and MAPK pathways.
Article Snippet: Funding information Rashtriya Uchchatar Shiksha Abhiyan RUSA 2.0, University of Madras, and Govt. of India Abstract Colorectal cancer (CRC) is among the most prevalent gastrointestinal cancers of epithelial origin worldwide, with over 2 million cases detected every year.. Emerging evidence suggests a significant increase in the levels of inflammatory and stress‐ related markers in patients with CRC, indicating that oxidative stress and lipid peroxidation may influence signalling cascades involved in the progression of the disease.. However, the precise molecular and cellular basis underlying CRC and their modulations during bioactive compound exposure have not yet been deciphered.

Article Title: FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination.
Article Snippet: Primary antibodies were incubated in 5% 18 Cell Reports 42, 113523, December 26, 2023 BLOT-QuickBlocker (G-Biosciences 786-011) as follows: mouse anti-PCNA (Abcam, ab29; 1:3000), rabbit anti-Ubiquityl-PCNA (Lys164) (Cell Signaling, D5C7P, 13439; 1:1000), rabbit anti-RPA32 (S33) (Bethyl, A300-246A; 1:2000), rabbit anti-gH2AX (Bethyl, A300-081A; 1:2000), rabbit anti-H2AX (Bethyl, A300-082A; 1:5000), rabbit anti-p-p53 (S15) (Cell Signaling, 9284S; 1:500), mouse anti-p53 (Santa Cruz, sc-126; 1:2000), rabbit anti-p21 (Santa Cruz, sc-397 clone C19; 1:1000), rabbit anti-FANCD2 (Abcam, ab108928; 1:2000), mouse anti-FANCL (Proteintech, 66639-1-Ig; 1:1,000), rabbit anti-RAD18 (Bethyl, A300-340A; 1:1000), rabbit anti-MCM2 (Cell Signaling, 4007S; 1:1000; BD Biosciences, 610701; 1:1000; Proteintech, 10513-1-AP; 1:1000), mouse anti-GAPDH (GeneTex, GTX627408; 1:10000), mouse anti-Ku86 (Santa Cruz, B-1, sc-5280; 1:500), mouse anti-a-tubulin (Millipore, T9026, clone DM1A; 1:10000), rabbit anti-ORC2 (Thermo Fisher, PA5-110063, 1:1000), rabbit anti-Histone H4 (Abcam, ab10158, 1:200,000).



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Image Search Results


Journal: iScience

Article Title: Hyperandrogenism triggers mtDNA release to participate in ovarian inflammation via mPTP/cGAS/STING in PCOS

doi: 10.1016/j.isci.2025.112391

Figure Lengend Snippet:

Article Snippet: QuickBlock TM Immunostaining blocking solution , Beyotime , Cat#P0260.

Techniques: Recombinant, Modification, Reverse Transcription, Immunostaining, Blocking Assay, DNA Extraction, Permeability, Sequencing, Software