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Beyotime quickblock western blocking solution
Quickblock Western Blocking Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblock+blocking+solution/quickblock+western/pm40359641-103-5-9
Average 90 stars, based on 1 article reviews

quickblock western blocking solution - by Bioz Stars, 2026-10

90/100 stars

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Incubation:

Article Title: Trem2 regulates microglial migratory responses via type I interferon signaling during photoreceptor degeneration.
Article Snippet: Ten micrograms of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (#1620177, Bio-Rad, Shanghai, China). .. Membranes were blocked with QuickBlockTM (#P0252, Beyotime) and incubated with primary and secondary antibodies (listed in Supplementary Table S1). .. Protein detection was performed using Enhanced chemiluminescence (ECL) reagent, and bands were visualized using a ChemiDoc imaging system (#1705061, Bio-Rad).

Article Title: SLC10A5 deficiency causes hypercholanemia
Article Snippet: The proteins were separated on SDS-PAGE gels and blotted onto PVDF membranes (Millipore). .. After being blocked with QuickBlock Western (Beyotime Biotechnology) for 0.5 h at room temperature, the PVDF membranes were incubated with primary antibodies at 4 °C overnight and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature after wash. .. The signal was visualized with electrochemiluminescence (Beyotime Biotechnology).

Article Title: Post-transcriptional regulation of diabetic wound healing by junctional adhesion molecule A/miR-106b axis.
Article Snippet: Background: Despite advancements in molecular science and biomaterial technology, the mechanisms underlying the impaired healing of diabetic wounds remain unclear.. In this study, we investigated the post-transcriptional regulation of diabetic wound healing using JAM-A.. Methods: Mouse wound models, hematoxylin and eosin staining analysis, and scratch wound assays were used to investigate the effects of JAM-A 3′-UTR on the re-epithelialization of diabetic wounds, whereas RNA pulldown, microRNA-seq, and bioinformatics analyses were performed to identify key miRNA players and predict their target genes.

Article Title: A Novel H 2 S Donor Alleviates Neuroinflammation and Seizures by Inhibiting the C3-C3aR Pathway.
Article Snippet: Both astrocytes and microglia are activated in the epileptic brain.. There is an interaction between them through the complement 3 (C3)C3a receptor (C3aR) pathway, which plays a detrimental role in seizures.. Our selfdeveloped novel H2S donor has been found to have antiseizure effects.

SDS Page:

Article Title: Both carvedilol and cimetidine alleviate cisplatin-induced nephrotoxicity via downregulating OCT2.
Article Snippet: Background: Cisplatin is a common chemotherapy agent for solid tumors but severe nephrotoxicity limits its application, with no effective pharmacological treatments.. Organic cation transporter 2 (OCT2) is involved in cisplatin uptake in kidneys.. This study aimed to find drugs with promising clinical applications that could prevent cisplatin-induced acute kidney injury (Cis-AKI) by inhibiting OCT2.

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish.
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. PLOS One | https://doi.org/10.1371/journal.pone.0324164 May 20, 2025 4 / 18 Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

Membrane:

Blocking Assay:

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

Western Blot:

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

Fat:

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

RNA Extraction:

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

Protein Concentration:

Article Title: Therapeutic potential of a choline-zinc-vitamin E nutraceutical complex in ameliorating thioacetamide-induced nonalcoholic fatty liver pathology in zebrafish
Article Snippet: The instruments used for the experiment are mainly as following: Anatomical microscope (SZX7, OLYMPUS, Japan); motorized focus continuous magnification fluorescence microscope (AZ100, Nikon, Japan); high-speed frozen centrifuge (Heraeus Fresco17, ThermoFisher, Germany); multi-functional enzyme labeler (Tecan, Spark, Switzerland); 6-well plate (Zhejiang Bellanber Biotechnology Co. China). .. Reagents and antibodies used in the experiment mainly included Dimethyl sulfoxide (DMSO, lot number: 20171016, Sinopharm Chemical Reagent Co., Ltd., China); Methyl cellulose (lot number: C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China); Fat-soluble diazol dye (lot number: SHBN4926, Sigma, USA); Thioacetamide (TAA, lot number: BCBV3031, Sigma, Switzerland); GPT Kit (ELISA) (lot number: 20220517, Nanjing Jiancheng Biological Research Institute, China); GOT Kit (ELISA) (lot number: 20220516, Nanjing Jiancheng Biological Research Institute, China); PBS buffer (Cat No. BL601A, Biosharp, China); 4% tissue cell fixative (lot number: 20210828, Beijing Solabao Technology Co. Ltd., China); FastKing cDNA First Strand Synthesis Kit (Degenome) (lot number: X1213, Tiangen Biochemical Technology (Beijing) Co., Ltd., China); Universal RNA Extraction TL Kit C (lot number: TL2204001643C, Foshan Aowei Biotechnology Co., Ltd., China); SDS-PAGE Gel Preparation Kit (lot number: 7E661H2, Vazyme, China); BCA Protein Concentration Assay Kit (lot number: AR1189, BOSTER, China); Western Quick Transfer Solution (10X) (lot number: P0572-2L, Beyotime, China); ECL Chemiluminescence Kit (lot number: G2014 -50ML, Service, China); QuickBlockTM Western Blocking Solution (lot number: P0252-500ml, Beyotime, China); Anti-β-Actin Antibody (lot number: BM0627, BOSTER, China); Primary Antibody Rabbit FPB2 Polyclonal Antibody (lot number: MBS320877, MyBioSourc, USA); Primary Antibody Acetyl-CoACarboxylas (lot number: CY5575, Shanghai Bowan Biotechnology Co., Ltd., China); Primary Antibody Rabbit ACSL1 Polyclonal Antibody (lot number: MBS320116, MyBioSourc, USA); primary antibody Rabbit TBC1D1 Polyclonal Antibody (lot number: MBS320992, MyBioSourc, USA); secondary antibody Goat Anti-Rabbit IgG (H&L) Biotin (lot number: BA1054, BOSTER, China). ..

other:

Article Title: Inhibition of HDAC3 induces neuroprotection by activating the Npas4 signaling pathway following surgical brain injury in rats
Article Snippet: The membrane was sealed using QuickBlock TM Western (Beyotime) and incubated at room temperature for 40 min, followed by overnight hybridization with the following primary antibodies in a 4 ° C refrigerator: HDAC3 (1:1,000, Cell Signaling Technology, USA, 3949S), Npas4 (1:1,000, Novus Biological, USA, NBP2-47252), LC3B (1:500, Boster Biological Technology, China, BM4827), Beclin-1 (1:500, Boster Biological Technology, China, BA3123-2), P62 (1:1,000, Abcam, UK, 5114S), IL-6 (1:1,000, ABclonal, USA, A0286), IL-1β (1:1,000, Abcam, UK, ab9787), TNF-α (1:1000, ABclonal, USA, A11534), albumin (1:1,000, Abcam, UK, ab207327), and GAPDH (1:5,000, Sigma, USA, G9545).

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