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quantscript reverse transcription kit  (tiangen biotech co)


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    Structured Review

    tiangen biotech co quantscript reverse transcription kit
    Quantscript Reverse Transcription Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 822 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantscript+reverse+transcription+rt+kit/QuantScript+RT+Kit/pm26496964-49-1-8
    Average 96 stars, based on 822 article reviews
    quantscript reverse transcription kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: The C-type Lysozyme of Miichthys miiuy Exhibits Specific Antibacterial Activity against Gram-negative Bacteria.
    Article Snippet: Lysozyme is a key antimicrobial molecule in the innate immune system of fish, playing a central role in defending against pathogen invasion.. In this study, a novel c-type lysozyme (mmi-LyzC) was identified and functionally characterized from Miichthys miiuy.. The full-length cDNA was 432 bp, encoding a 143-amino-acid protein containing a conserved LYZ_C domain, in which the active sites Glu50 and Asp67 were highly conserved across teleosts.

    Article Title: Food-based multisensory stimulation ameliorates cognitive impairment after mild traumatic brain injury in male rats by modulating intestinal and brain inflammation.
    Article Snippet: .. Total RNA was extracted using TRIzol Reagent (CWBIO) and reverse-transcribed with the Quantscript RT Kit (Tiangen Biotech Co.). .. PCR reactions were performed in a 20 μL volume using the SYBR Premix Ex Taq Kit (Applied Biological Materials Inc.) on a 7900 HT Fast Real-Time PCR System (Applied Biosystems).

    Article Title: Food-based multisensory stimulation ameliorates cognitive impairment after mild traumatic brain injury in male rats by modulating intestinal and brain inflammation
    Article Snippet: .. Total RNA was extracted using TRIzol Reagent (CWBIO) and reverse-transcribed with the Quantscript RT Kit (Tiangen Biotech Co.). .. PCR reactions were performed in a 20 μL volume using the SYBR Premix Ex Taq Kit (Applied Biological Materials Inc.) on a 7900 HT Fast Real-Time PCR System (Applied Biosystems).

    other:

    Article Title: The conserved long noncoding RNA LTCONS12135 positively regulates innate immunity in teleost.
    Article Snippet: Long noncoding RNAs (lncRNAs) have been widely demonstrated to be key regulators of innate immunity in mammals, but their roles in antimicrobial and antiviral immune responses in aquatic animals have not been well described.. In this study, a novel lncRNA, LTCONS12135, was identified in the miiuy croaker (Miichthys miiuy) and was found to be responsive to innate immune stimulation and to participate in both antibacterial and antiviral immune responses.. The expression of LTCONS12135 was significantly upregulated in M. miiuy spleen cells (MSpC) following stimulation with lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (poly(I:C)).

    Synthesized:

    Article Title: Truncation of CYR1 Promoter in Baker’s Yeast to Improve Freeze Tolerance
    Article Snippet: Total RNA was extracted using the Fungal mRNA Out Kit (Tiandz Biotech, Beijing, China). .. First-strand cDNA was synthesized using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) with oligo (dT) primers at 37 ◦C for 1 h. Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green PCR Kit (Tiangen Biotech, Beijing, China) to determine https://doi.org/10.3390/fermentation12080373 the relative mRNA levels of CYR1 and ACT1 (the latter serving as an internal control), with the primer pairs CYR1-F/CYR1-R and ACT1-F/ACT1-R, respectively. .. All qRT-PCR reactions were run on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland).

    Article Title: The Impact of Mitochondrial DNA Depletion on Mitochondrial Ultrastructure, Photosynthesis, and the mTERF Gene Family in Chlamydomonas reinhardtii
    Article Snippet: .. First-strand cDNA was synthesized [ ] from 500 ng of total RNA using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) in a 20 μL reaction volume. ..

    Article Title: The Impact of Mitochondrial DNA Depletion on Mitochondrial Ultrastructure, Photosynthesis, and the mTERF Gene Family in Chlamydomonas reinhardtii .
    Article Snippet: .. First-strand cDNA was synthesized [50] from 500 ng of total RNA using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) in a 20 μL reaction volume. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Truncation of CYR1 Promoter in Baker’s Yeast to Improve Freeze Tolerance
    Article Snippet: Total RNA was extracted using the Fungal mRNA Out Kit (Tiandz Biotech, Beijing, China). .. First-strand cDNA was synthesized using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) with oligo (dT) primers at 37 ◦C for 1 h. Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green PCR Kit (Tiangen Biotech, Beijing, China) to determine https://doi.org/10.3390/fermentation12080373 the relative mRNA levels of CYR1 and ACT1 (the latter serving as an internal control), with the primer pairs CYR1-F/CYR1-R and ACT1-F/ACT1-R, respectively. .. All qRT-PCR reactions were run on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland).

    SYBR Green Assay:

    Article Title: Truncation of CYR1 Promoter in Baker’s Yeast to Improve Freeze Tolerance
    Article Snippet: Total RNA was extracted using the Fungal mRNA Out Kit (Tiandz Biotech, Beijing, China). .. First-strand cDNA was synthesized using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) with oligo (dT) primers at 37 ◦C for 1 h. Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green PCR Kit (Tiangen Biotech, Beijing, China) to determine https://doi.org/10.3390/fermentation12080373 the relative mRNA levels of CYR1 and ACT1 (the latter serving as an internal control), with the primer pairs CYR1-F/CYR1-R and ACT1-F/ACT1-R, respectively. .. All qRT-PCR reactions were run on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland).

    Polymerase Chain Reaction:

    Article Title: Truncation of CYR1 Promoter in Baker’s Yeast to Improve Freeze Tolerance
    Article Snippet: Total RNA was extracted using the Fungal mRNA Out Kit (Tiandz Biotech, Beijing, China). .. First-strand cDNA was synthesized using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) with oligo (dT) primers at 37 ◦C for 1 h. Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green PCR Kit (Tiangen Biotech, Beijing, China) to determine https://doi.org/10.3390/fermentation12080373 the relative mRNA levels of CYR1 and ACT1 (the latter serving as an internal control), with the primer pairs CYR1-F/CYR1-R and ACT1-F/ACT1-R, respectively. .. All qRT-PCR reactions were run on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland).

    Control:

    Article Title: Truncation of CYR1 Promoter in Baker’s Yeast to Improve Freeze Tolerance
    Article Snippet: Total RNA was extracted using the Fungal mRNA Out Kit (Tiandz Biotech, Beijing, China). .. First-strand cDNA was synthesized using the Quantscript RT Kit (Tiangen Biotech, Beijing, China) with oligo (dT) primers at 37 ◦C for 1 h. Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green PCR Kit (Tiangen Biotech, Beijing, China) to determine https://doi.org/10.3390/fermentation12080373 the relative mRNA levels of CYR1 and ACT1 (the latter serving as an internal control), with the primer pairs CYR1-F/CYR1-R and ACT1-F/ACT1-R, respectively. .. All qRT-PCR reactions were run on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland).



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