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quantigene sample processing kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher quantigene sample processing kit
    Quantigene Sample Processing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantigene+kit/quantigene+sample+processing+kit/pm40430554-634-14-25
    Average 90 stars, based on 1 article reviews
    quantigene sample processing kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Effects of short-chain per- and polyfluoroalkyl substances (PFAS) on toxicologically relevant gene expression profiles in a liver-on-a-chip model.
    Article Snippet: Short-chain perand polyfluoroalkyl substances (PFAS) are highly stable and widely used environmental contaminants that pose potential health risks to humans.. Aggregating reliable mechanistic information for safety assessments necessitates physiologically relevant high-throughput screening approaches.. Here, we demonstrated the utility of a liver-on-a-chip model to investigate the effects of five short-chain PFAS at low (1 nM) and high (1 μM) concentrations on toxicologically-relevant gene expression profiles using the QuantiGene® Plex Assay.

    Cell Culture:

    Article Title: Ribosome subunit attrition and activation of the p53–MDM4 axis dominate the response of MLL-rearranged cancer cells to WDR5 WIN site inhibition
    Article Snippet: Commercial assay or kit , QuantiGene Plex panel , Thermo Fisher Scientific , NA , . .. Commercial assay or kit , QuantiGene Sample Processing Kit for cultured cells , Thermo Fisher Scientific , Cat# QS0100 , . .. Commercial assay or kit , QuantiGene Plex Assay kits , Thermo Fisher Scientific , Cat# QP1013 , .

    Article Title: Ribosome subunit attrition and activation of the p53–MDM4 axis dominate the response of MLL-rearranged cancer cells to WDR5 WIN site inhibition
    Article Snippet: .. A custom QuantiGene Plex panel (ThermoFisher Scientific) was used in conjunction with the QuantiGene Sample Processing Kit for cultured cells (ThermoFisher Scientific), and QuantiGene Plex Assay kit (ThermoFisher Scientific) to quantify transcripts following the manufacturer’s instructions. ..

    Gene Expression:

    Article Title: Antibodies that bind interleukin 13 and methods of use
    Article Snippet: .. Cells remaining in the assay plates were lysed and mRNA extracted for analysis of NTRK1 gene expression using a commercial Quantigene kit (ThermoFisher). .. Levels of NTRK1 mRNA were determined according to the manufacturer's protocol and analyzed in GraphPad Prism.

    Plex Assay:

    Article Title: Ribosome subunit attrition and activation of the p53–MDM4 axis dominate the response of MLL-rearranged cancer cells to WDR5 WIN site inhibition
    Article Snippet: .. A custom QuantiGene Plex panel (ThermoFisher Scientific) was used in conjunction with the QuantiGene Sample Processing Kit for cultured cells (ThermoFisher Scientific), and QuantiGene Plex Assay kit (ThermoFisher Scientific) to quantify transcripts following the manufacturer’s instructions. ..

    Expressing:

    Article Title: Activating PKC-ε induces HIV expression with improved tolerability
    Article Snippet: .. EGR1 and CD69 mRNA expression levels were measured in whole blood, processed using Affymetrix QuantiGene Sample Processing Kit (Thermo Fisher Scientific, catalog QS0111). .. Quantigene multiplex analysis was performed on 1:3 diluted blood using probes specific for rat EGR1 (Thermo Fisher Scientific, catalog nm_012551) and CD69 (catalog nm_134327), as per the kit’s instructions (Thermo Fisher Scientific, catalog QP-1014) and normalized to PPIB (catalog nm_022536) [ ] and β-actin (catalog nm_031144).



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    Thermo Fisher quantigene viewrna mirna cell assay kit
    ( A ) Schematic representation of the workflow to obtain PBMCs samples from the FOR2107 cohort (see also Methods and Protocols section). Subjects underwent psychiatric and cognitive evaluation. After the data was collected, blood was withdrawn and PBMCs prepared followed by RNA extraction and downstream gene expression analysis. Samples were collected from psychiatrically healthy female subjects (CTL), or psychiatrically healthy female subjects with genetic predisposition for mood disorder (genetic risk – GR) or that suffered from childhood maltreatment (environmental risk – ER) (Vulnerability samples). ( B ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs ER ( n = 18) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( C ) Table showing the top 15 differentially expressed miRNAs from CTL vs ER subjects, ordered from smallest to largest p -value. <t>miRNA-708-5p</t> is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( D ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs GR ( n = 14) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( E ) Table showing the top 15 differentially expressed miRNAs from CTL vs GR subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( F ) miR-708-5p qPCR analysis of total RNA isolated from PBMCs of CTL ( n = 18), ER ( n = 16), and GR ( n = 18) subjects. One-way ANOVA with Holm-Sidak’s multiple comparisons test, CTL vs ER, ** P = 0.0064; CTL vs GR, *** P = 0.0006; ER vs GR, ns. Data are presented as violin plots with median, quartiles and data points. See also: Fig. . .
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    Image Search Results


    ( A ) Schematic representation of the workflow to obtain PBMCs samples from the FOR2107 cohort (see also Methods and Protocols section). Subjects underwent psychiatric and cognitive evaluation. After the data was collected, blood was withdrawn and PBMCs prepared followed by RNA extraction and downstream gene expression analysis. Samples were collected from psychiatrically healthy female subjects (CTL), or psychiatrically healthy female subjects with genetic predisposition for mood disorder (genetic risk – GR) or that suffered from childhood maltreatment (environmental risk – ER) (Vulnerability samples). ( B ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs ER ( n = 18) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( C ) Table showing the top 15 differentially expressed miRNAs from CTL vs ER subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( D ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs GR ( n = 14) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( E ) Table showing the top 15 differentially expressed miRNAs from CTL vs GR subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( F ) miR-708-5p qPCR analysis of total RNA isolated from PBMCs of CTL ( n = 18), ER ( n = 16), and GR ( n = 18) subjects. One-way ANOVA with Holm-Sidak’s multiple comparisons test, CTL vs ER, ** P = 0.0064; CTL vs GR, *** P = 0.0006; ER vs GR, ns. Data are presented as violin plots with median, quartiles and data points. See also: Fig. . .

    Journal: EMBO Reports

    Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice

    doi: 10.1038/s44319-025-00410-y

    Figure Lengend Snippet: ( A ) Schematic representation of the workflow to obtain PBMCs samples from the FOR2107 cohort (see also Methods and Protocols section). Subjects underwent psychiatric and cognitive evaluation. After the data was collected, blood was withdrawn and PBMCs prepared followed by RNA extraction and downstream gene expression analysis. Samples were collected from psychiatrically healthy female subjects (CTL), or psychiatrically healthy female subjects with genetic predisposition for mood disorder (genetic risk – GR) or that suffered from childhood maltreatment (environmental risk – ER) (Vulnerability samples). ( B ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs ER ( n = 18) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( C ) Table showing the top 15 differentially expressed miRNAs from CTL vs ER subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( D ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs GR ( n = 14) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( E ) Table showing the top 15 differentially expressed miRNAs from CTL vs GR subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( F ) miR-708-5p qPCR analysis of total RNA isolated from PBMCs of CTL ( n = 18), ER ( n = 16), and GR ( n = 18) subjects. One-way ANOVA with Holm-Sidak’s multiple comparisons test, CTL vs ER, ** P = 0.0064; CTL vs GR, *** P = 0.0006; ER vs GR, ns. Data are presented as violin plots with median, quartiles and data points. See also: Fig. . .

    Article Snippet: QuantiGene ViewRNA miRNA Cell Assay Kit , Thermo Fisher , QVCM0001.

    Techniques: RNA Extraction, Gene Expression, RNA Sequencing, Isolation, Labeling, Quantitative Proteomics

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice

    doi: 10.1038/s44319-025-00410-y

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: QuantiGene ViewRNA miRNA Cell Assay Kit , Thermo Fisher , QVCM0001.

    Techniques: Recombinant, Expressing, Plasmid Preparation, Sequencing, Cloning, Software, Microscopy, Protease Inhibitor, Western Blot, cDNA Synthesis, SYBR Green Assay, Reverse Transcription, Lysis, Luciferase