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quantigene sample processing kit ffpe tissues  (Thermo Fisher)


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    Thermo Fisher quantigene sample processing kit ffpe tissues
    Quantigene Sample Processing Kit Ffpe Tissues, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 2009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantigene+ffpe+sample+processing+kit/GeneJET+FFPE+DNA+Purification+Kit/pmc06233536-100-11-13
    Average 98 stars, based on 2009 article reviews
    quantigene sample processing kit ffpe tissues - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Study on Spatio-temporal Prevalence and Hematological Attributes of Bovine Babesiosis in Cattle Population of Layyah, Southern Punjab, Pakistan
    Article Snippet: Hemoglobin concentration (Hb), Total Erythrocyte Count (TEC), Packed Cell Volume (PCV), Total Leukocyte Count (TLC), Neutrophils, Eosinophils, Basophils, Monocytes and Lymphocytes were analyzed through a veterinary hematology analyzer (Vet Exigo, H400, Boule Diagnostics AB, Sweden). .. DNA extraction was carried out using a commercial kit (Gene Jet Whole Blood DNA Purification Kit, Thermo-fisher Scientific, USA; catalogue no. K0782) following the procedure described by the manufacturer. .. Briefly, 200μL of whole blood sample was added in a 1.5mL eppendorf tube along with 400μL of lysis solution and 20μL of Proteinase-K.

    DNA Purification:

    Article Title: Study on Spatio-temporal Prevalence and Hematological Attributes of Bovine Babesiosis in Cattle Population of Layyah, Southern Punjab, Pakistan
    Article Snippet: Hemoglobin concentration (Hb), Total Erythrocyte Count (TEC), Packed Cell Volume (PCV), Total Leukocyte Count (TLC), Neutrophils, Eosinophils, Basophils, Monocytes and Lymphocytes were analyzed through a veterinary hematology analyzer (Vet Exigo, H400, Boule Diagnostics AB, Sweden). .. DNA extraction was carried out using a commercial kit (Gene Jet Whole Blood DNA Purification Kit, Thermo-fisher Scientific, USA; catalogue no. K0782) following the procedure described by the manufacturer. .. Briefly, 200μL of whole blood sample was added in a 1.5mL eppendorf tube along with 400μL of lysis solution and 20μL of Proteinase-K.

    other:

    Article Title: CCAAT/Enhancer-Binding Protein-α Suppresses Lung Tumor Development in Mice through the p38α MAP Kinase Pathway
    Article Snippet: Selected tumor samples on slide from paraffin embedded sections were isolated and RNA extraction was performed using the FFPE RNeasy-kit (Invitrogen).

    Isolation:

    Article Title: MicroRNA Signatures in Tumor Tissue Related to Angiogenesis in Non-Small Cell Lung Cancer
    Article Snippet: .. RNA was isolated from the collected core samples by using the Recover AllTM Total Nucleic Acid Isolation Kit for FFPE Tissues (Ambion, Austin, TX), according to the manufacturer's instructions, with some minor adjustments. ..

    Article Title: Stromal Expression of MiR-21 Predicts Biochemical Failure in Prostate Cancer Patients with Gleason Score 6
    Article Snippet: .. RNA was isolated from formalin-fixed paraffin-embedded samples with RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissue (Life Technologies), and was sent to Exiqon (Vedbaek, Denmark) which performed the miRNA screening experiment. ..

    Article Title: Hypoxia-Induced Down-Regulation of microRNA-34a Promotes EMT by Targeting the Notch Signaling Pathway in Tubular Epithelial Cells
    Article Snippet: .. Total RNA was extracted from the FFPE tissue cores using the Ambion Recover All Total Nucleic Acid Isolation Kit for FFPE tissues (Applied Biosystems/Ambion, Austin TX, USA) according to the manufacturer's instructions with a modified digestion time (5 hours). ..

    Article Title: Differences in microRNA expression during tumor development in the transition and peripheral zones of the prostate
    Article Snippet: .. In short, total RNA was extracted using the RecoverAll total nucleic acid isolation kit optimized for FFPE tissues (Ambion) before reverse transcription using the TaqMan® MicroRNA reverse transcription kit and MegaplexTM RT primers, human pool v2.0 (Applied Biosystems). .. The cDNA samples were pre-amplified using MegaplexTM PreAmp primers and TaqMan® Preamp master mix (Applied Biosystems) and then diluted in a 0.1X TE Buffer (pH 8.0) before use in the qPCR reaction.

    Formalin-fixed Paraffin-Embedded:

    Article Title: MicroRNA Signatures in Tumor Tissue Related to Angiogenesis in Non-Small Cell Lung Cancer
    Article Snippet: .. RNA was isolated from the collected core samples by using the Recover AllTM Total Nucleic Acid Isolation Kit for FFPE Tissues (Ambion, Austin, TX), according to the manufacturer's instructions, with some minor adjustments. ..

    Article Title: Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development
    Article Snippet: .. Sensation plus FFPE WT kit (Affymetrix, USA) was used for all 12 samples following the manufacturer’s protocol. .. Upon hybridization, the chip was scanned using Affymetrix GeneChip Scanner 3000.

    Article Title: Stromal Expression of MiR-21 Predicts Biochemical Failure in Prostate Cancer Patients with Gleason Score 6
    Article Snippet: .. RNA was isolated from formalin-fixed paraffin-embedded samples with RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissue (Life Technologies), and was sent to Exiqon (Vedbaek, Denmark) which performed the miRNA screening experiment. ..

    Article Title: Hypoxia-Induced Down-Regulation of microRNA-34a Promotes EMT by Targeting the Notch Signaling Pathway in Tubular Epithelial Cells
    Article Snippet: .. Total RNA was extracted from the FFPE tissue cores using the Ambion Recover All Total Nucleic Acid Isolation Kit for FFPE tissues (Applied Biosystems/Ambion, Austin TX, USA) according to the manufacturer's instructions with a modified digestion time (5 hours). ..

    Article Title: Differences in microRNA expression during tumor development in the transition and peripheral zones of the prostate
    Article Snippet: .. In short, total RNA was extracted using the RecoverAll total nucleic acid isolation kit optimized for FFPE tissues (Ambion) before reverse transcription using the TaqMan® MicroRNA reverse transcription kit and MegaplexTM RT primers, human pool v2.0 (Applied Biosystems). .. The cDNA samples were pre-amplified using MegaplexTM PreAmp primers and TaqMan® Preamp master mix (Applied Biosystems) and then diluted in a 0.1X TE Buffer (pH 8.0) before use in the qPCR reaction.

    Modification:

    Article Title: Hypoxia-Induced Down-Regulation of microRNA-34a Promotes EMT by Targeting the Notch Signaling Pathway in Tubular Epithelial Cells
    Article Snippet: .. Total RNA was extracted from the FFPE tissue cores using the Ambion Recover All Total Nucleic Acid Isolation Kit for FFPE tissues (Applied Biosystems/Ambion, Austin TX, USA) according to the manufacturer's instructions with a modified digestion time (5 hours). ..

    Purification:

    Article Title: Field Longevity of a Fluorescent Protein Marker in an Engineered Strain of the Pink Bollworm, Pectinophora gossypiella (Saunders)
    Article Snippet: At the conclusion of the study, two traps from each period were sent, chilled, to Oxitec laboratories in the UK for PCR analysis. .. From each period, genomic DNA was extracted (GeneJET® genomic purification kit, Fermentas) from 75 moths (one whole trap plus 15 moths randomly selected from other traps using www.random.org ), and each sample was genotyped by PCR for the presence of two sequences ( ) [2 min at 94°C, 3× (10 s at 95°C, 1 min at 62°C, 2 min at 72°C), 27× (10 s at 95°C, 30 s at 62°C, 55 s at 72°C), and 5 min at 72°C] - one spanning the 5′ junction of the OX1138B insertion (primers 5′-CTGCTCGGGCGAGCGTATATAGAC-3′ and 5′-CTCTGGACGTCATCTTCACTTACGTG-3′ ) and the other spanning the wild-type genomic insertion site of the transgene (amplifies a fragment when no transgene is present; primers 5′-CTGCTCGGGCGAGCGTATATAGAC-3′ and 5′-CCGCCGTCATTTCTACATTAGTAAGA-3′ ) - which we used to identify OX1138B and APHIS moths, respectively. ..

    Polymerase Chain Reaction:

    Article Title: Field Longevity of a Fluorescent Protein Marker in an Engineered Strain of the Pink Bollworm, Pectinophora gossypiella (Saunders)
    Article Snippet: At the conclusion of the study, two traps from each period were sent, chilled, to Oxitec laboratories in the UK for PCR analysis. .. From each period, genomic DNA was extracted (GeneJET® genomic purification kit, Fermentas) from 75 moths (one whole trap plus 15 moths randomly selected from other traps using www.random.org ), and each sample was genotyped by PCR for the presence of two sequences ( ) [2 min at 94°C, 3× (10 s at 95°C, 1 min at 62°C, 2 min at 72°C), 27× (10 s at 95°C, 30 s at 62°C, 55 s at 72°C), and 5 min at 72°C] - one spanning the 5′ junction of the OX1138B insertion (primers 5′-CTGCTCGGGCGAGCGTATATAGAC-3′ and 5′-CTCTGGACGTCATCTTCACTTACGTG-3′ ) and the other spanning the wild-type genomic insertion site of the transgene (amplifies a fragment when no transgene is present; primers 5′-CTGCTCGGGCGAGCGTATATAGAC-3′ and 5′-CCGCCGTCATTTCTACATTAGTAAGA-3′ ) - which we used to identify OX1138B and APHIS moths, respectively. ..

    Reverse Transcription:

    Article Title: Differences in microRNA expression during tumor development in the transition and peripheral zones of the prostate
    Article Snippet: .. In short, total RNA was extracted using the RecoverAll total nucleic acid isolation kit optimized for FFPE tissues (Ambion) before reverse transcription using the TaqMan® MicroRNA reverse transcription kit and MegaplexTM RT primers, human pool v2.0 (Applied Biosystems). .. The cDNA samples were pre-amplified using MegaplexTM PreAmp primers and TaqMan® Preamp master mix (Applied Biosystems) and then diluted in a 0.1X TE Buffer (pH 8.0) before use in the qPCR reaction.



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    Selection of FFPE specimens from HNSCC patients for the biomarker expression study using QG-MPH. Out of 100 HNSCC cases, 97 were assigned. The specimens were tested by H&E staining for tumor tissue residues. A total of 25 cases were excluded due to non-sufficient tumor tissue. MPG and QG-MPH assays were performed with FFPE sections of 72 specimens, and 68 were valid for further analysis. Abbreviation: FFPE = formalin-fixed-paraffin-embedded; HNSCC = head and neck squamous cell carcinoma; H&E staining = hematoxylin and eosin staining; MPG = Multiplex Papillomavirus Genotyping assay; QG-MPH = QuantiGene-Molecular-Profiling-Histology assay; IHC = immunohistochemistry.

    Journal: International Journal of Molecular Sciences

    Article Title: Identifying Truly HPV-Driven Head and Neck Squamous Cell Carcinoma by QuantiGene-Molecular-Profiling-Histology Assay Allows for More Precise Prognosis Prediction

    doi: 10.3390/ijms252413643

    Figure Lengend Snippet: Selection of FFPE specimens from HNSCC patients for the biomarker expression study using QG-MPH. Out of 100 HNSCC cases, 97 were assigned. The specimens were tested by H&E staining for tumor tissue residues. A total of 25 cases were excluded due to non-sufficient tumor tissue. MPG and QG-MPH assays were performed with FFPE sections of 72 specimens, and 68 were valid for further analysis. Abbreviation: FFPE = formalin-fixed-paraffin-embedded; HNSCC = head and neck squamous cell carcinoma; H&E staining = hematoxylin and eosin staining; MPG = Multiplex Papillomavirus Genotyping assay; QG-MPH = QuantiGene-Molecular-Profiling-Histology assay; IHC = immunohistochemistry.

    Article Snippet: RNA was released from FFPE sections (5 μm thickness, 5–10 pieces) using the QuantiGene FFPE Sample Processing Kit (ThermoFisher, Cat. #QS0109, Waltham, MA, USA) according to the manufacturer’s protocol.

    Techniques: Selection, Biomarker Assay, Expressing, Staining, Formalin-fixed Paraffin-Embedded, Multiplex Assay, Genotyping Assay, Immunohistochemistry