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quantigene sample processing kits ffpe tissues  (Thermo Fisher)


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    Structured Review

    Thermo Fisher quantigene sample processing kits ffpe tissues
    Quantigene Sample Processing Kits Ffpe Tissues, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantigene+ffpe+sample+processing+kit/pm30537722-47-22-29
    Average 90 stars, based on 1 article reviews
    quantigene sample processing kits ffpe tissues - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: P3.12-05 The Pattern of PD-L1 Expression in Thoracic Neuroendocrine Tumors
    Article Snippet: .. Method: RNA was isolated from two fragments of the same tumor and its paired lymph node metastasis, using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissues (Ambion/Thermo Fisher). miRNA profiling was performed using the TaqMan Array Human microRNA card A v.3.0 platform (Life Technologies/Thermo Fisher). miRNA expression was analyzed in the Expression Suite software using the global normalization algorithm provided. ..

    Article Title: Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer
    Article Snippet: .. Total RNA were extracted from FFPE specimens using Total Nucleic Acid Isolation Kits for FFPE tissues (Ambion, Austin, TX, USA). ..

    Article Title: Deregulation of miR-100, miR-99a and miR-199b in tissues and plasma coexists with increased expression of mTOR kinase in endometrioid endometrial carcinoma
    Article Snippet: 40 to 80 mg of macro dissected tissues stored in RNAlater was homogenized in a rotor -stator homogenizer and RNA was isolated using mirVANA TM miRNA Isolation Kit (Ambion) according to manufacturer’s protocol. .. RNA isolation from FFPE tissues was performed using Recover AllTM Total Nucleic Acid Isolation Kit for FFPE Tissues (Ambion) according to the protocol provided by the manufacturer. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: P3.12-05 The Pattern of PD-L1 Expression in Thoracic Neuroendocrine Tumors
    Article Snippet: .. Method: RNA was isolated from two fragments of the same tumor and its paired lymph node metastasis, using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissues (Ambion/Thermo Fisher). miRNA profiling was performed using the TaqMan Array Human microRNA card A v.3.0 platform (Life Technologies/Thermo Fisher). miRNA expression was analyzed in the Expression Suite software using the global normalization algorithm provided. ..

    Article Title: VEGF-A , VEGFR1 and VEGFR2 single nucleotide polymorphisms and outcomes from the AGITG MAX trial of capecitabine, bevacizumab and mitomycin C in metastatic colorectal cancer
    Article Snippet: .. RNA was extracted and converted into cDNA and gene expression profiling performed using the Almac Xcel Array (Almac, Craigavon, UK), utilising Affymetrix GeneChip technology optimised for use with FFPE tissue. .. For analysis of protein expression levels, protein lysates were prepared from the FFPE tumour sections and quantified using the EZQ Protein Quantitation Kit (Life Technologies, Carlsbad, CA, USA).

    Article Title: Expression Levels of microRNAs miR-93 and miR-200a in Pancreatic Adenocarcinoma with Special Reference to Differentiation and Relapse-Free Survival.
    Article Snippet: Objectives: Protein levels of the transcription factor nuclear factor erythroid-derived 2-like 2 (Nrf2) and its inhibitor Kelchlike ECH-associated protein 1 (Keap1) have been proposed as prognostic factors in pancreatic ductal adenocarcinomas (PDACs).. These cellular redox-state-regulating enzymes are targeted by several microRNAs, including miR-93 and miR200a.. Methods: We assessed mRNA levels of Nrf2 and Keap1 and tissue expression of miR-93 and miR-200a in 51 patients with surgically treated PDAC.

    Article Title: Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer
    Article Snippet: .. Total RNA were extracted from FFPE specimens using Total Nucleic Acid Isolation Kits for FFPE tissues (Ambion, Austin, TX, USA). ..

    Article Title: Identification of stromal ColXα1 and tumor-infiltrating lymphocytes as putative predictive markers of neoadjuvant therapy in estrogen receptor-positive/HER2-positive breast cancer
    Article Snippet: .. RNA was purified using the RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) and further purified and concentrated with the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA). .. RNA was isolated and purified using the RNeasy FFPE kit (Qiagen, Valencia, CA, USA).

    Article Title: Deregulation of miR-100, miR-99a and miR-199b in tissues and plasma coexists with increased expression of mTOR kinase in endometrioid endometrial carcinoma
    Article Snippet: 40 to 80 mg of macro dissected tissues stored in RNAlater was homogenized in a rotor -stator homogenizer and RNA was isolated using mirVANA TM miRNA Isolation Kit (Ambion) according to manufacturer’s protocol. .. RNA isolation from FFPE tissues was performed using Recover AllTM Total Nucleic Acid Isolation Kit for FFPE Tissues (Ambion) according to the protocol provided by the manufacturer. ..

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: .. RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) were used for extraction. .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously .

    Expressing:

    Article Title: P3.12-05 The Pattern of PD-L1 Expression in Thoracic Neuroendocrine Tumors
    Article Snippet: .. Method: RNA was isolated from two fragments of the same tumor and its paired lymph node metastasis, using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissues (Ambion/Thermo Fisher). miRNA profiling was performed using the TaqMan Array Human microRNA card A v.3.0 platform (Life Technologies/Thermo Fisher). miRNA expression was analyzed in the Expression Suite software using the global normalization algorithm provided. ..

    Software:

    Article Title: P3.12-05 The Pattern of PD-L1 Expression in Thoracic Neuroendocrine Tumors
    Article Snippet: .. Method: RNA was isolated from two fragments of the same tumor and its paired lymph node metastasis, using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissues (Ambion/Thermo Fisher). miRNA profiling was performed using the TaqMan Array Human microRNA card A v.3.0 platform (Life Technologies/Thermo Fisher). miRNA expression was analyzed in the Expression Suite software using the global normalization algorithm provided. ..

    Gene Expression:

    Article Title: VEGF-A , VEGFR1 and VEGFR2 single nucleotide polymorphisms and outcomes from the AGITG MAX trial of capecitabine, bevacizumab and mitomycin C in metastatic colorectal cancer
    Article Snippet: .. RNA was extracted and converted into cDNA and gene expression profiling performed using the Almac Xcel Array (Almac, Craigavon, UK), utilising Affymetrix GeneChip technology optimised for use with FFPE tissue. .. For analysis of protein expression levels, protein lysates were prepared from the FFPE tumour sections and quantified using the EZQ Protein Quantitation Kit (Life Technologies, Carlsbad, CA, USA).

    Extraction:

    Article Title: INTUSSUSCEPTIVE-LIKE ANGIOGENESIS IN HUMAN FETAL LUNG XENOGRAFTS: LINK WITH BRONCHOPULMONARY DYSPLASIA-ASSOCIATED MICROVASCULAR DYSANGIOGENESIS?
    Article Snippet: .. Total RNA was extracted from the captured areas using the RecoverAll Total Nucleic Acid Extraction kits for formalin-fixed, paraffin-embedded (FFPE) tissues (Ambion, Austin, TX). .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup kit (Qiagen, Valencia, CA) and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies, Santa Clara, CA).

    Article Title: Identification of stromal ColXα1 and tumor-infiltrating lymphocytes as putative predictive markers of neoadjuvant therapy in estrogen receptor-positive/HER2-positive breast cancer
    Article Snippet: .. RNA was purified using the RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) and further purified and concentrated with the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA). .. RNA was isolated and purified using the RNeasy FFPE kit (Qiagen, Valencia, CA, USA).

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: .. RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) were used for extraction. .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously .

    Purification:

    Article Title: Identification of stromal ColXα1 and tumor-infiltrating lymphocytes as putative predictive markers of neoadjuvant therapy in estrogen receptor-positive/HER2-positive breast cancer
    Article Snippet: .. RNA was purified using the RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) and further purified and concentrated with the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA). .. RNA was isolated and purified using the RNeasy FFPE kit (Qiagen, Valencia, CA, USA).



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    Selection of FFPE specimens from HNSCC patients for the biomarker expression study using QG-MPH. Out of 100 HNSCC cases, 97 were assigned. The specimens were tested by H&E staining for tumor tissue residues. A total of 25 cases were excluded due to non-sufficient tumor tissue. MPG and QG-MPH assays were performed with FFPE sections of 72 specimens, and 68 were valid for further analysis. Abbreviation: FFPE = formalin-fixed-paraffin-embedded; HNSCC = head and neck squamous cell carcinoma; H&E staining = hematoxylin and eosin staining; MPG = Multiplex Papillomavirus Genotyping assay; QG-MPH = QuantiGene-Molecular-Profiling-Histology assay; IHC = immunohistochemistry.

    Journal: International Journal of Molecular Sciences

    Article Title: Identifying Truly HPV-Driven Head and Neck Squamous Cell Carcinoma by QuantiGene-Molecular-Profiling-Histology Assay Allows for More Precise Prognosis Prediction

    doi: 10.3390/ijms252413643

    Figure Lengend Snippet: Selection of FFPE specimens from HNSCC patients for the biomarker expression study using QG-MPH. Out of 100 HNSCC cases, 97 were assigned. The specimens were tested by H&E staining for tumor tissue residues. A total of 25 cases were excluded due to non-sufficient tumor tissue. MPG and QG-MPH assays were performed with FFPE sections of 72 specimens, and 68 were valid for further analysis. Abbreviation: FFPE = formalin-fixed-paraffin-embedded; HNSCC = head and neck squamous cell carcinoma; H&E staining = hematoxylin and eosin staining; MPG = Multiplex Papillomavirus Genotyping assay; QG-MPH = QuantiGene-Molecular-Profiling-Histology assay; IHC = immunohistochemistry.

    Article Snippet: RNA was released from FFPE sections (5 μm thickness, 5–10 pieces) using the QuantiGene FFPE Sample Processing Kit (ThermoFisher, Cat. #QS0109, Waltham, MA, USA) according to the manufacturer’s protocol.

    Techniques: Selection, Biomarker Assay, Expressing, Staining, Formalin-fixed Paraffin-Embedded, Multiplex Assay, Genotyping Assay, Immunohistochemistry