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Becton Dickinson pseap2-basic plasmid
Pseap2 Basic Plasmid, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pseap2-basic+plasmid/pseap2+basic+vector/pmc01479809-93-11-13
Average 90 stars, based on 1 article reviews
pseap2-basic plasmid - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Inhibition of Hepatitis C Virus in Chimeric Mice by Short Synthetic Hairpin RNAs: Sequence Analysis of Surviving Virus Shows Added Selective Pressure of Combination Therapy
Article Snippet: Thirteen nanograms of pIRES/fLuc 130 (an IRES-linked firefly luciferase reporter construct), 20 ng of pSEAP2-control plasmid (BD 131 7 Biosciences Clontech, San Jose, CA) as a transfection control, and the indicated amounts of 132 sshRNAs were co-transfected into the 293FT cells.

Article Title: Molecular Cloning of Rat and Porcine Retina-derived POU Domain Factor 1 (POU6F2) from a Pituitary cDNA Library
Article Snippet: The expression vector of Rpf1 in pcDNA3.1, and the serial truncated upstream regions of the mouse Prop1 (NC_000077.6), Prrx1 (NC_000067.6) and Prrx2 (NC_000068.7) genes were amplified using primers ( ) and were ligated to the secreted alkaline phosphatase (SEAP) plasmid vector pSEAP2-Basic (BD Biosciences Clontech, Palo Alto, CA, USA).

Article Title: Otx2 ChIP-seq Reveals Unique and Redundant Functions in the Mature Mouse Retina
Article Snippet: Transcriptional activity of the Otx2 and Crx proteins was assayed using the region –66 to +68 of the IRBP promoter cloned into the pSEAP2-basic vector (BD Biosciences, Palo Alto, CA, USA).

Article Title: Highly sensitive and specific bioassay for measuring bioactive TGF-β
Article Snippet: This fragment was then ligated into the NheI/HindIII site of the pSEAP2-Basic plasmid (BD Biosciences, San Jose, CA).

Article Title: Expression of Krüppel-Like Factor 6, KLF6, in Rat Pituitary Stem/Progenitor Cells and Its Regulation of the PRRX2 Gene
Article Snippet: Upstream regions of the mouse Prrx1 (NC_000067.6) and Prrx2 (NC_000068.7) genes were amplified using primers ( ) and were ligated to the secreted alkaline phosphatase (SEAP) plasmid vector pSEAP2-Basic (BD Biosciences Clontech, Palo Alto, CA, USA).

Article Title: Retinoic acid signalling is a candidate regulator of the expression of pituitary-specific transcription factor Prop1 in the developing rodent pituitary.
Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/jne.12570 This article is protected by copyright.. All rights reserved.

ALP Activity Assay:

Article Title: Keratinocyte Growth Factor and Glucocorticoid Induction of Human Peroxiredoxin 6 Gene Expression Occur by Independent Mechanisms That Are Synergistic
Article Snippet: Statistical significance was determined by one-way ANOVA or t -test as appropriate using SigmaStat (Jandell Scientific, San Rafael, CA). p -values of <0.05 were considered significant. .. Minimal essential medium (MEM) was from Life Technologies (Grand Island, NY), OptiMEM was from Invitrogen, (Carlsbad, CA), KGF and Dex were from (Sigma-Aldrich, St. Louis, MO). pSEAP2 alkaline phosphatase reporter vectors and an alkaline phosphatase activity assay kit were from BD Bioscience (San Jose, CA). .. Competent cells, restriction enzymes, and the Beta-Glo assay system were from Promega (Madison, WI).

Article Title: Keratinocyte Growth Factor and Glucocorticoid Induction of Human Peroxiredoxin 6 Gene Expression Occur by Independent Mechanisms That Are Synergistic
Article Snippet: .. Chemicals and reagents Minimal essential medium (MEM) was from Life Technologies (Grand Island, NY), OptiMEM was from Invitrogen, (Carlsbad, CA), KGF and Dex were from (Sigma-Aldrich, St. Louis, MO). pSEAP2 alkaline phosphatase reporter vectors and an alkaline phosphatase activity assay kit were from BD Bioscience (San Jose, CA). .. Competent cells, restriction enzymes, and the Beta-Glo assay system were from Promega (Madison, WI).



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