primescript rt kit (TaKaRa)
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primescript rt kit
Primescript Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10983 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pm42844252-463-15-22
Average 98 stars, based on 10983 article reviews
Primescript Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10983 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pm42844252-463-15-22
Average 98 stars, based on 10983 article reviews
primescript rt kit - by Bioz Stars,
2026-10
98/100 stars
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Reverse Transcription:Article Title: A core transcriptional regulatory circuitry controls super-enhancer-driven activation of LGR5 in colorectal cancer. Article Snippet: Total RNA was extracted by TRIzol reagent (Life Technologies) according to the manufacturer’s instructions. .. One microgram of RNA was used for each reverse transcription reaction, which was set by Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis. Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined. Article Title: Decitabine induces cell differentiation and growth inhibition accompanied by upregulation of microRNA-143 and MS4A3 in the myelodysplastic syndromes-derived leukemic cell line MDS-L. Article Snippet: RNA was extracted from cells using a RNeasy Mini Kit (Qiagen Sciences, Inc.). .. Total RNA was reverse transcribed to cDNA using a Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus. Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus). cDNA Synthesis:Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells. Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS. SYBR Green Assay:Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells. Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS. Polymerase Chain Reaction:Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells. Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS. Reverse Transcription Polymerase Chain Reaction:Article Title: The capsid proteins of orthoflavivirus are interchangeable for West Nile virus assembly. Article Snippet: .. Total RNAs of WNV-DV2mC-P10-A/B/C-infected cells were extracted, and RT-PCR was then carried out using the Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis. Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined. Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus. Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus). Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy Article Snippet: .. Measurements of host gene expression were performed using Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using One Step RT-PCR:Article Title: The capsid proteins of orthoflavivirus are interchangeable for West Nile virus assembly. Article Snippet: .. Total RNAs of WNV-DV2mC-P10-A/B/C-infected cells were extracted, and RT-PCR was then carried out using the Real-time Polymerase Chain Reaction:Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis. Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined. Gene Expression:Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy Article Snippet: .. Measurements of host gene expression were performed using Concentration Assay:Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using Spectrophotometry:Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using Synthesized:Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using |