primescript rt master mix kit (TaKaRa)
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primescript rt master mix kit
Primescript Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+kit/PrimeScript+RT+Master+Mix/pmc13656048-118-12-17
Average 99 stars, based on 26966 article reviews
Primescript Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+kit/PrimeScript+RT+Master+Mix/pmc13656048-118-12-17
Average 99 stars, based on 26966 article reviews
primescript rt master mix kit - by Bioz Stars,
2026-10
99/100 stars
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Reverse Transcription:Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease Article Snippet: Total RNA was extracted from tissues or cells using TRIzol reagent (Invitrogen, USA). .. Reverse transcription was performed using Article Title: Ac NR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis Article Snippet: Worm morphology was observed using a stereomicroscopy (Leica, Germany), and the size of eggs was calculated using Image J. .. Total RNA was extracted from adult worms using Trizol (Invitrogen, USA) and reverse transcribed into cDNA using the Article Title: PRRSV infection induces mitochondrial damage and releases mitochondrial dsRNA to activate the NLRP3-dependent inflammatory response. Article Snippet: Total RNA was extracted from the collected cells following the manufacturer’s instructions (Invitrogen). .. The RNA concentration was adjusted to 50 ng/ μL, and reverse transcription was performed using the Article Title: AcNR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis. Article Snippet: Worm morphology was observed using a stereomicroscopy (Leica, Germany), and the size of eggs was calculated using Image J. .. Total RNA was extracted from adult worms using Trizol (Invitrogen, USA) and reverse transcribed into cDNA using the Article Title: Bone Marrow Macrophage STING Signaling is a Direct Nexus Linking Marrow Adipose Expansion to NAD + Depletion and Osteogenic Decline in Skeletal Aging Article Snippet: .. Total RNA was extracted from cells using TRNzol Universal (TIANGEN, DP424) and reverse‐transcribed into cDNA using the Concentration Assay:Article Title: PRRSV infection induces mitochondrial damage and releases mitochondrial dsRNA to activate the NLRP3-dependent inflammatory response. Article Snippet: Total RNA was extracted from the collected cells following the manufacturer’s instructions (Invitrogen). .. The RNA concentration was adjusted to 50 ng/ μL, and reverse transcription was performed using the Generated:Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats Article Snippet: Total RNA was purified using TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol. .. Complementary DNA (cDNA) was generated from equal amounts of RNA using the other:Article Title: Carmn sustains basal stemness in exocrine glands and CTND1-mediated Wnt signaling in mammary tissue. Article Snippet: Reagents and tools table Reagent or resource Source Identifier Reagent or resource Source Identifier Antibodies Anti-CD45, fluorophore-conjugated Thermo Fisher #11-0451-81 Anti-CD31, fluorophoreconjugated Thermo Fisher #11-0311-81 Anti-Ter119, fluorophore-conjugated Thermo Fisher #11-5921-82 Anti-CD49f, fluorophore-conjugated R&D Systems #FAB13501P Anti-CD24, fluorophore-conjugated BD Biosciences #562349 Anti-Keratin 14 (Krt14), used for RNA-FISH costaining FN (as written in manuscript) #FNab02195 Anti-alpha smooth muscle actin, used for RNA-FISH co-staining Proteintech #23081-1-AP Anti-p120 Catenin, used for RNA-FISH costaining Proteintech #66208-1-Ig Anti-Ki67, organoid immunofluorescence/ tissue IHC Abcam #ab15580 Anti-Krt8 Cohesionbio #CPA1659 Anti-Krt14, tissue immunofluorescence FineTest #FNab02195 Anti-Acta2, tissue immunofluorescence Proteintech #14395-1-AP Anti-p120 Catenin, tissue immunofluorescence Proteintech #66208-1-Ig Anti-TWIST1 Affinity/Affinity Biosciences #AF4009 Anti-CTND1 Bethyl Laboratories #A300-978A-T Anti-Flag CST #14793 Anti-HA CST #3724 Anti-beta-catenin, ChIP Abcam #ab32572 Secondary fluorescence antibodies Abcam; Signalway Antibody Goat anti-Rabbit 488, ab150077; Goat antiRabbit 594, L3017; Goat anti-Rabbit 647, © The Author(s) EMBO reports 11 Reagent or resource Source Identifier L35451; Goat anti-Rat 594, ab150160 Chemicals, peptides, recombinant proteins, and culture reagents Collagenase/ Hyaluronidase STEMCELL Technologies #07912 EpiCult-B supplement STEMCELL Technologies #05611 EpiCult-B basal medium STEMCELL Technologies #05610 NH4Cl (red blood cell removal) STEMCELL Technologies 07850 Trypsin-EDTA, 0.25% TransGen FG301-01 Dispase, MaSC dissociation STEMCELL Technologies #07913 DNase I STEMCELL Technologies #07900 Advanced DMEM/F-12 Gibco #12634010 Matrigel Corning Life Sciences/ Corning #354234 Epidermal growth factor (EGF) Sigma #E9644 FGF2, MaSC culture EMD Millipore #GF003 Heparin ExcellBio #FSP500 Y-27632 R&D Systems #1254 CHIR, MaSC culture InvivoChem #V0211 Dispase, lacrimal epithelial stem cell isolation (25 U/mL) STEMCELL Technologies #07913 Collagenase I Biosharp #bs163 N2 supplement Gibco #17502048 B27 supplement Gibco #17504044 GlutaMAX Gibco #35050061 FGF10 PeproTech #100-26 Wnt3a, lacrimal epithelial stem cell culture PeproTech #315-20 DMEM/F-12, salivary epithelial stem cell culture Gibco 12634010 Type II collagenase Sigma-Aldrich C2-28-100MG FGF2, salivary epithelial stem cell culture Novoprotein #C046 Wnt3a, salivary epithelial stem cell culture Novoprotein #C22R Nicotinamide Yuanye Bio #S13015 TGF-beta inhibitor A8301 KKL med #KM2595 CHIR, salivary epithelial stem cell culture InvivoChem #V2805 DMEM/F-12, mammary tumor organoid digestion Gibco #12634010 Trypsin-EDTA, 0.25% GENOM GNM25200-5 Reagent or resource Source Identifier Dispase, mammary tumor organoid digestion buffer II STEMCELL Technologies (written as Stemcell) #07923 DMEM GENOM #GNM12320 Antibiotics for HEK293/ HEK293T culture TransGen Penicillin/ Streptomycin, FG101-01 Polybrene Yeasen #40804ES76 Lipofectamine 2000 invitrogen 52887 GSK-3 inhibitor BIO Calbiochem #361552 Dispase, organoid immunofluorescence (5 U/mL) STEMCELL Technologies 07913 Paraformaldehyde (PFA), 4% fixation Biosharp BL539A Triton X-100 Solarbio T8200 BSA BioFroxx 4240GR100 DAPI Solarbio C0060 Fructose-glycerol clearing/mounting medium Prepared in-house from D-(-)-fructose (Sangon) and glycerol (Macklin) Fructose A6002130500; glycerol G810575 Ethanol Sinopharm 64-17-5 Chloroform Sinopharm 67-66-3 Glacial acetic acid Sinopharm 10000208 Carmine Sigma 237086 Aluminum potassium sulfate dodecahydrate Sigma C1022-25G Xylene Sinopharm 1330-20-7 Methyl salicylate D&B K898001 Glycerol Macklin G810575 Hematoxylin Servicebio G1004 Hydrochloric ethanol differentiator solution Servicebio G1039 Eosin solution Servicebio G1001 Neutral mounting glue Solarbio Neutral balsam, G8590 TRIzol Thermo Fisher #15596026 Protease inhibitor cocktail MCE #HY-K0010 Glycine Solarbio G8200-5kg PBS GENOM GNM20012-5 Phenol-chloroform BioFlux BSA03M1 DMEM for mammary stem cell transplantation mixture GENOM DMEM high glucose, GNM12800-5 Critical commercial assays, kits, probes, and |