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primerscript reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa primerscript reverse transcriptase
    Primerscript Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primerscript+reverse+transcriptase+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc12296446-47-0-4
    Average 99 stars, based on 2413 article reviews
    primerscript reverse transcriptase - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Human febrile illness associated with Yanggou tick virus: Clinical characteristics and epidemiological links to rodents.
    Article Snippet: .. Viral RNA in questing ticks, and samples from wild rodents and human patients was extracted using a TIANamp Virus RNA Kit (TIANGEN, China) before being reverse transcribed into cDNA with the PrimeScript 1st Strand cDNA Synthesis kit (TaKaRa, Japan) following the manufacturer’s instructions. ..

    Reverse Transcription:

    Article Title: Human febrile illness associated with Yanggou tick virus: Clinical characteristics and epidemiological links to rodents.
    Article Snippet: .. Viral RNA in questing ticks, and samples from wild rodents and human patients was extracted using a TIANamp Virus RNA Kit (TIANGEN, China) before being reverse transcribed into cDNA with the PrimeScript 1st Strand cDNA Synthesis kit (TaKaRa, Japan) following the manufacturer’s instructions. ..

    Article Title: Differential expression of arsenic transporters across growth stages determines arsenic accumulation in rice cultivars.
    Article Snippet: 1 ICAR National Rice Research Institute, Cuttack, Odisha 753006, India 2 School of Agriculture, DRIEMS University, Cuttack, Odisha 754022, India 3 Institute of Environment and Sustainable Development, BHU, Varanasi, India 4 Global Centre for Environmental Remediation (GCER), School of Sciences, The University of Newcastle, Callaghan, NSW 2308, Australia 5 Department of Ecological Studies, International Centre for Ecological Engineering, University of Kalyani, Kalyani, West Bengal 741235, India 6 Indian Council of Agricultural Research, New Delhi 110001, India Received: 12 March 2026 / Revised: 7 August 2026 / Accepted: 1 September 2026 © Prof. H.S.. Srivastava Foundation for Science and Society 2026 Differential expression of arsenic transporters across growth stages determines arsenic accumulation in rice cultivars

    Article Title: SUMO2 deletion changes chromatin accessibility and enhances cytotoxic T cell activation and tumor infiltration
    Article Snippet: Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. .. Total RNA concentration was determined using a NanoDrop (Invitrogen) by measuring absorbance at 260/280 nm, and 1 μg of RNA was used for reverse transcription with the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio). .. For qPCR, 100 ng of cDNA from each sample was amplified with gene-specific primers using Power SYBR Green PCR Master Mix (Applied Biosystems) and QuantStudio 5 Real-Time PCR Systems (Applied Biosystems).

    Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol.
    Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation.

    Article Title: Engineered mesenchymal stem cell-derived small extracellular vesicles delivering miR-124 suppress colorectal cancer progression through EZH2 downregulation and attenuation of STAT3 signaling.
    Article Snippet: 1 Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran 2 Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Abstract Colorectal cancer (CRC) progression is driven by dysregulated molecular pathways and epigenetic alterations, including enhancer of zeste homolog 2 (EZH2)-mediated histone methylation and STAT3 hyperactivation.. While microRNA-124 (miR-124) acts as a tumor suppressor by targeting EZH2, its clinical translation is hindered by delivery challenges.. Here, we engineered mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) to deliver miR-124, exploiting their tumor-homing capacity and biocompatibility to suppress CRC progression.

    Article Title: Evidence of a redox-dependent regulation of satellite cell activity and regeneration in human skeletal muscle following aseptic injury: a randomized controlled trial.
    Article Snippet: Satellite cells are essential for skeletal muscle regeneration, with their activity tightly regulated by glutathione (GSH) metabolism and redox balance.. Effective regeneration requires an optimal redox hormetic range and balanced GSH levels, as both oxidative and reductive distress can impair redox signaling, satellite cell differentiation, and muscle repair.. Despite its significance, redox-regulated regeneration responses in human skeletal muscle remain underexplored.

    cDNA Synthesis:

    Article Title: Human febrile illness associated with Yanggou tick virus: Clinical characteristics and epidemiological links to rodents.
    Article Snippet: .. Viral RNA in questing ticks, and samples from wild rodents and human patients was extracted using a TIANamp Virus RNA Kit (TIANGEN, China) before being reverse transcribed into cDNA with the PrimeScript 1st Strand cDNA Synthesis kit (TaKaRa, Japan) following the manufacturer’s instructions. ..

    Article Title: SUMO2 deletion changes chromatin accessibility and enhances cytotoxic T cell activation and tumor infiltration
    Article Snippet: Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. .. Total RNA concentration was determined using a NanoDrop (Invitrogen) by measuring absorbance at 260/280 nm, and 1 μg of RNA was used for reverse transcription with the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio). .. For qPCR, 100 ng of cDNA from each sample was amplified with gene-specific primers using Power SYBR Green PCR Master Mix (Applied Biosystems) and QuantStudio 5 Real-Time PCR Systems (Applied Biosystems).

    Article Title: Molecular evidence of aquaporin-4 dysregulation and neuroinflammatory stress underlying blood-brain barrier dysfunction in idiopathic intracranial hypertension: A case-control study.
    Article Snippet: .. RNA was extracted from blood and CSF samples using Qiagen QIAamp RNA Blood Mini Kit (cat. no. 52304)17 and Qiagen miRNeasy Advanced Kit (cat. no. 217204) (Qiagen N.V. Venlo, Netherlands).18 cDNA synthesis was performed immediately after RNA isolation using Takara cDNA Synthesis Kit (cat. no. 6110A)19 and stored at −20°C until the day for RTPCR. .. Primers were designed on National Center for Biotechnology Information database (NCBI)20 and validated using in- silico PCR21 (Table S1).

    Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol.
    Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation.

    Article Title: Engineered mesenchymal stem cell-derived small extracellular vesicles delivering miR-124 suppress colorectal cancer progression through EZH2 downregulation and attenuation of STAT3 signaling.
    Article Snippet: 1 Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran 2 Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Abstract Colorectal cancer (CRC) progression is driven by dysregulated molecular pathways and epigenetic alterations, including enhancer of zeste homolog 2 (EZH2)-mediated histone methylation and STAT3 hyperactivation.. While microRNA-124 (miR-124) acts as a tumor suppressor by targeting EZH2, its clinical translation is hindered by delivery challenges.. Here, we engineered mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) to deliver miR-124, exploiting their tumor-homing capacity and biocompatibility to suppress CRC progression.

    Article Title: Evidence of a redox-dependent regulation of satellite cell activity and regeneration in human skeletal muscle following aseptic injury: a randomized controlled trial.
    Article Snippet: Satellite cells are essential for skeletal muscle regeneration, with their activity tightly regulated by glutathione (GSH) metabolism and redox balance.. Effective regeneration requires an optimal redox hormetic range and balanced GSH levels, as both oxidative and reductive distress can impair redox signaling, satellite cell differentiation, and muscle repair.. Despite its significance, redox-regulated regeneration responses in human skeletal muscle remain underexplored.

    Article Title: Immune responses and seasonal red sea bream iridovirus dynamics following formalin-inactivated vaccination in farmed rock bream (Oplegnathus fasciatus).
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Concentration Assay:

    Article Title: SUMO2 deletion changes chromatin accessibility and enhances cytotoxic T cell activation and tumor infiltration
    Article Snippet: Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. .. Total RNA concentration was determined using a NanoDrop (Invitrogen) by measuring absorbance at 260/280 nm, and 1 μg of RNA was used for reverse transcription with the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio). .. For qPCR, 100 ng of cDNA from each sample was amplified with gene-specific primers using Power SYBR Green PCR Master Mix (Applied Biosystems) and QuantStudio 5 Real-Time PCR Systems (Applied Biosystems).

    Clinical Proteomics:

    Article Title: Molecular evidence of aquaporin-4 dysregulation and neuroinflammatory stress underlying blood-brain barrier dysfunction in idiopathic intracranial hypertension: A case-control study.
    Article Snippet: .. RNA was extracted from blood and CSF samples using Qiagen QIAamp RNA Blood Mini Kit (cat. no. 52304)17 and Qiagen miRNeasy Advanced Kit (cat. no. 217204) (Qiagen N.V. Venlo, Netherlands).18 cDNA synthesis was performed immediately after RNA isolation using Takara cDNA Synthesis Kit (cat. no. 6110A)19 and stored at −20°C until the day for RTPCR. .. Primers were designed on National Center for Biotechnology Information database (NCBI)20 and validated using in- silico PCR21 (Table S1).

    Isolation:

    Article Title: Molecular evidence of aquaporin-4 dysregulation and neuroinflammatory stress underlying blood-brain barrier dysfunction in idiopathic intracranial hypertension: A case-control study.
    Article Snippet: .. RNA was extracted from blood and CSF samples using Qiagen QIAamp RNA Blood Mini Kit (cat. no. 52304)17 and Qiagen miRNeasy Advanced Kit (cat. no. 217204) (Qiagen N.V. Venlo, Netherlands).18 cDNA synthesis was performed immediately after RNA isolation using Takara cDNA Synthesis Kit (cat. no. 6110A)19 and stored at −20°C until the day for RTPCR. .. Primers were designed on National Center for Biotechnology Information database (NCBI)20 and validated using in- silico PCR21 (Table S1).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Molecular evidence of aquaporin-4 dysregulation and neuroinflammatory stress underlying blood-brain barrier dysfunction in idiopathic intracranial hypertension: A case-control study.
    Article Snippet: .. RNA was extracted from blood and CSF samples using Qiagen QIAamp RNA Blood Mini Kit (cat. no. 52304)17 and Qiagen miRNeasy Advanced Kit (cat. no. 217204) (Qiagen N.V. Venlo, Netherlands).18 cDNA synthesis was performed immediately after RNA isolation using Takara cDNA Synthesis Kit (cat. no. 6110A)19 and stored at −20°C until the day for RTPCR. .. Primers were designed on National Center for Biotechnology Information database (NCBI)20 and validated using in- silico PCR21 (Table S1).

    Real-time Polymerase Chain Reaction:

    Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol.
    Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation.

    Analysis:

    Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol.
    Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation.

    Cloning:

    Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol.
    Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation.

    Synthesized:

    Article Title: Immune responses and seasonal red sea bream iridovirus dynamics following formalin-inactivated vaccination in farmed rock bream (Oplegnathus fasciatus).
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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