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ppcrscript vector  (Agilent technologies)


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    Structured Review

    Agilent technologies ppcrscript vector
    Ppcrscript Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/us07769575-134-5-7
    Average 90 stars, based on 1 article reviews
    ppcrscript vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Three dimensional coordinates of HPTPbeta
    Article Snippet: The intracellular domain (ICD) of HPTPbeta (SEQ ID NO: 3) is cloned from human fetal heart cDNA containing the full gene of HPTPbeta (SEQ ID NO: 1) by PCR using Advantage Polymerase (Clontech) and the primers: Beta-FOR2: 5′-GATCGACCATTATCTGTCCAC-3′ SEQ ID NO: 8 Beta-REV2: 5′-CAGGAGCTCTTCAGGTACAT-3′ SEQ ID NO: 9 under the following reaction conditions: 1 cycle at 95° C. for 1 minute, 30 cycles at 94° C. for 30 seconds and 62° C. for 2 minutes, and a final cycle of 62° C. for 3 minutes. .. PCR products are subcloned into pPCRScript vector (Stratagene) and sequenced, revealing 2 silent mutations in the HPTPbeta ICD clone, one at base pair (bp) 5372 (C to T, a Glycine residue) and the other at bp 5895 (C to T, a Tyrosine residue) (nucleotides are numbered from bp #1 in SEQ ID #1, which means that the initiator methionine in SEQ ID #2 corresponds to the codon beginning at base pair #31 in SEQ ID NO 1). .. The sequence [SEQ ID NO: 5] encoding base pairs 5014 to 5949 of SEQ ID NO: 1 is cloned into the vector pMALc2x (New England Biolabs) using the following oligonucleotides: SEQ ID NO: 10 5′primer: 5′-CGAGCATACGTAGATCGACCATTATCTGTCC-3′ SEQ ID NO: 11 3′primer: 5′-CGAGCAAGCTTATTATTGTTCACTCCGTAGC-3′ The HPTPbeta truncated gene [SEQ ID NO: 5] (Wang, Y.

    Plasmid Preparation:

    Article Title: Three dimensional coordinates of HPTPbeta
    Article Snippet: The intracellular domain (ICD) of HPTPbeta (SEQ ID NO: 3) is cloned from human fetal heart cDNA containing the full gene of HPTPbeta (SEQ ID NO: 1) by PCR using Advantage Polymerase (Clontech) and the primers: Beta-FOR2: 5′-GATCGACCATTATCTGTCCAC-3′ SEQ ID NO: 8 Beta-REV2: 5′-CAGGAGCTCTTCAGGTACAT-3′ SEQ ID NO: 9 under the following reaction conditions: 1 cycle at 95° C. for 1 minute, 30 cycles at 94° C. for 30 seconds and 62° C. for 2 minutes, and a final cycle of 62° C. for 3 minutes. .. PCR products are subcloned into pPCRScript vector (Stratagene) and sequenced, revealing 2 silent mutations in the HPTPbeta ICD clone, one at base pair (bp) 5372 (C to T, a Glycine residue) and the other at bp 5895 (C to T, a Tyrosine residue) (nucleotides are numbered from bp #1 in SEQ ID #1, which means that the initiator methionine in SEQ ID #2 corresponds to the codon beginning at base pair #31 in SEQ ID NO 1). .. The sequence [SEQ ID NO: 5] encoding base pairs 5014 to 5949 of SEQ ID NO: 1 is cloned into the vector pMALc2x (New England Biolabs) using the following oligonucleotides: SEQ ID NO: 10 5′primer: 5′-CGAGCATACGTAGATCGACCATTATCTGTCC-3′ SEQ ID NO: 11 3′primer: 5′-CGAGCAAGCTTATTATTGTTCACTCCGTAGC-3′ The HPTPbeta truncated gene [SEQ ID NO: 5] (Wang, Y.

    Residue:

    Article Title: Three dimensional coordinates of HPTPbeta
    Article Snippet: The intracellular domain (ICD) of HPTPbeta (SEQ ID NO: 3) is cloned from human fetal heart cDNA containing the full gene of HPTPbeta (SEQ ID NO: 1) by PCR using Advantage Polymerase (Clontech) and the primers: Beta-FOR2: 5′-GATCGACCATTATCTGTCCAC-3′ SEQ ID NO: 8 Beta-REV2: 5′-CAGGAGCTCTTCAGGTACAT-3′ SEQ ID NO: 9 under the following reaction conditions: 1 cycle at 95° C. for 1 minute, 30 cycles at 94° C. for 30 seconds and 62° C. for 2 minutes, and a final cycle of 62° C. for 3 minutes. .. PCR products are subcloned into pPCRScript vector (Stratagene) and sequenced, revealing 2 silent mutations in the HPTPbeta ICD clone, one at base pair (bp) 5372 (C to T, a Glycine residue) and the other at bp 5895 (C to T, a Tyrosine residue) (nucleotides are numbered from bp #1 in SEQ ID #1, which means that the initiator methionine in SEQ ID #2 corresponds to the codon beginning at base pair #31 in SEQ ID NO 1). .. The sequence [SEQ ID NO: 5] encoding base pairs 5014 to 5949 of SEQ ID NO: 1 is cloned into the vector pMALc2x (New England Biolabs) using the following oligonucleotides: SEQ ID NO: 10 5′primer: 5′-CGAGCATACGTAGATCGACCATTATCTGTCC-3′ SEQ ID NO: 11 3′primer: 5′-CGAGCAAGCTTATTATTGTTCACTCCGTAGC-3′ The HPTPbeta truncated gene [SEQ ID NO: 5] (Wang, Y.



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