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ppcr script vector  (Agilent technologies)


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    Structured Review

    Agilent technologies ppcr script vector
    Ppcr Script Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcr+script+vector/pm36614101-397-24-27
    Average 90 stars, based on 1 article reviews
    ppcr script vector - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Process and materials for production of glucosamine and N-acetylglucosamine
    Article Snippet: The resulting fragment was ligated into vector pPCR-ScriptTMSK(+) (Stratagene Cloning Systems, La Jolla, Calif.), generating plasmid pKLN07-14.

    Article Title: Cloning and functional expression of a liver isoform of the small conductance Ca2+-activated K+ channel SK3.
    Article Snippet: Barfod, Elisabeth T., Ann L. Moore, and Steven D. Lidofsky.. Cloning and functional expression of a liver isoform of the small conductance Ca-activated K channel SK3.. Am J Physiol Cell Physiol 280: C836–C842, 2001.— Small conductance Ca-activated K (SK) channels have been cloned from mammalian brain, but little is known about the molecular characteristics of SK channels in nonexcitable tissues.

    Article Title: Process and materials for production of glucosamine and N-acetylglucosamine
    Article Snippet: The resulting ̃3.2 kb PCR fragment was ligated into vector pPCR-ScriptTMSK(+) (Stratagene Cloning Systems, La Jolla, Calif.), generating plasmids pSW07-53 #7 and #17.

    Article Title: Process and materials for production of glucosamine and N-acetylglucosamine
    Article Snippet: The resulting 4.7-kb PCR fragment containing the araBAD operon was ligated into vector pPCR-ScriptTMSK(+) (Stratagene Cloning Systems, La Jolla, Calif.), generating plasmid pKLN07-38.

    Article Title: Process and materials for production of glucosamine and N-acetylglucosamine
    Article Snippet: 171(9):4617-4622, herein by reference of its entirety) and the kanamycin resistance cassette of plasmid pUC4K (Amersham Pharmacia Biotech, Piscataway, N.J.) was excised from plasmid pKLN07-21 using restriction enzymes Not I and Kpn I. Plasmid pKLN07-21 was constructed by PCR amplification of the temperature sensitive replicon from plasmid pSW07-4 (described in Example 6), ligation of the PCR product into the vector pPCR-ScriptTMSK(+) (Stratagene Cloning Systems, La Jolla, Calif.), and addition of the kanamycin resistance cassette from plasmid pUC4K (Amersham Pharmacia Biotech, Piscataway, N.J.).

    Amplification:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response.
    Article Snippet: .. 2023, 24, 656 17 of 21 was amplified from the genomic DNA with primers NT90/NT91 [22] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using XhoI/XbaI and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Clone Assay:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response.
    Article Snippet: .. 2023, 24, 656 17 of 21 was amplified from the genomic DNA with primers NT90/NT91 [22] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using XhoI/XbaI and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Plasmid Preparation:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response.
    Article Snippet: .. 2023, 24, 656 17 of 21 was amplified from the genomic DNA with primers NT90/NT91 [22] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using XhoI/XbaI and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Construct:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.



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