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Serumwerk Bernburg AG polymorphprep gradient
Polymorphprep Gradient, supplied by Serumwerk Bernburg AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+gradient/polymorphprep/pm38161237-48-9-11
Average 90 stars, based on 1 article reviews
polymorphprep gradient - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Neutrophil single-cell analysis identifies a type II interferon-related subset for predicting relapse of autoimmune small vessel vasculitis
Article Snippet: White blood cells were separated using Polymorphprep (Serumwerk, Cat. No. 1895) before flow cutometry experiments.

Article Title: Neutrophil single-cell analysis identifies a type II interferon-related subset for predicting relapse of autoimmune small vessel vasculitis.
Article Snippet: White blood cells were separated using Polymorphprep (Serumwerk, Cat. No. 1895).

Isolation:

Article Title: Aryl hydrocarbon receptor, a promising therapeutic target for ulcerative colitis, impairs HK2-controlled flux of hexosamine-biosynthesis pathway to eliminate NETosis in an N-glycosylation dependent manner.
Article Snippet: Colonic tissues from mice with colitis were homogenised, and MPO activity was measured using a commercial kit (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. .. Neutrophils were isolated from the peripheral blood of participants, as approved by the Ethics Committee of the Affiliated Hospital of Integrated Traditional Chinese 8 and Western Medicine (Approval No. 2022-LWKY-035), using PolymorphprepTM (Serumwerk Bernburg AG, Germany) according to the manufacturer’s instructions. .. The human promyelocytic leukaemia cell line HL60 was obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in Iscove’s Modified Dulbecco’s Medium supplemented with 20% fetal bovine serum at 37°C.

Article Title: Anesthetic choice impacts mortality and bacterial clearance in a murine experimental pneumonia model.
Article Snippet: Blood was drawn from consenting healthy volunteers into 10 mL heparin tubes (BD Biosciences). .. Whole blood was used directly, while neutrophils were isolated using Polymorphprep (Serumwerk Bernburg AG for Alere Technologies, AS, Oslo, Norway) as described elsewhere [9]. ..

Article Title: Neutrophil extracellular traps impede cancer metastatic seeding via protease-activated receptor 2-mediated downregulation of phagocytic checkpoint CD24.
Article Snippet: On the day of the cancer cell injection, 25 mg/kg cetuximab (CTX, Merck Millipore, Darmstadt, Germany, Cat# S20171039) was injected once i.p. .. Human neutrophils were isolated from the peripheral blood of healthy donors using Polymorphprep (Serumwerk Bernburg AG, Germany, Cat1895) according to the manufacturer’s instructions. ..

Article Title: Anesthetic choice impacts mortality and bacterial clearance in a murine experimental pneumonia model
Article Snippet: Blood was drawn from consenting healthy volunteers into 10 mL heparin tubes (BD Biosciences). .. Whole blood was used directly, while neutrophils were isolated using Polymorphprep (Serumwerk Bernburg AG for Alere Technologies, AS, Oslo, Norway) as described elsewhere [ ]. ..

Article Title: Single-cell analysis reveals immune cell abnormalities underlying the clinical heterogeneity of patients with systemic sclerosis.
Article Snippet: .. Nature Communications | (2025) 16:4949 13 Two milliliters of Whole blood was collected from the same patient in an EDTA-2Na blood collection tube (Terumo, Cat. No. VPNa052K), and the leukocytes were isolated using Polymorphprep (Serumwerk, Cat. No. 1895). .. The sample was washed and resuspended with Cellbanker 1plus (ZENOAQ, Cat. No. CB023) to a concentration of 2.0 × 106 cell/mL prior to scRNA-seq experiments.

Western Blot:

Article Title: Aryl hydrocarbon receptor, a promising therapeutic target for ulcerative colitis, impairs HK2-controlled flux of hexosamine-biosynthesis pathway to eliminate NETosis in an N-glycosylation dependent manner.
Article Snippet: Colonic tissues from mice with colitis were homogenised, and MPO activity was measured using a commercial kit (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. .. Neutrophils were isolated from the peripheral blood of participants, as approved by the Ethics Committee of the Affiliated Hospital of Integrated Traditional Chinese 8 and Western Medicine (Approval No. 2022-LWKY-035), using PolymorphprepTM (Serumwerk Bernburg AG, Germany) according to the manufacturer’s instructions. .. The human promyelocytic leukaemia cell line HL60 was obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in Iscove’s Modified Dulbecco’s Medium supplemented with 20% fetal bovine serum at 37°C.

Gentle:

Article Title: Type 1 innate lymphoid cell–immature neutrophil axis suppresses acute tissue inflammation
Article Snippet: .. Human umbilical cord blood was overlayed onto PolymorphPrep (Serumwerk bernburg, Bernburg, Germany) and was centrifuged at 450 x g for 35 min at 21 °C with a gentle break. ..



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