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substrate prepared from the pathhunter flash detection kit  (DiscoverX corporation)

 
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    Structured Review

    DiscoverX corporation substrate prepared from the pathhunter flash detection kit
    Substrate Prepared From The Pathhunter Flash Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathhunter+detection+kit/pathhunter+flash+detection+kit/us12201633-503-3-11
    Average 90 stars, based on 1 article reviews
    substrate prepared from the pathhunter flash detection kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Cannabinoid receptor modulators
    Article Snippet: Assay Readout: β-Arrestin assay readout was accomplished using a PHERAstar (BMG LABTECH Inc., Durham, N.C.) or EnVision (PerkinElmer, Fremont Calif.) microplate reader.

    Article Title: Cannabinoid receptor modulators
    Article Snippet: The assay: The stable CHO-K1 cells expressing CB2—Prolink fusion protein were plated over night in 384-well plates (Optiplate 384-Plus, PerkinElmer, Fremont Calif.; catalog #6007299) at 5000 cells/5 μL/well in the Opti-MEM medium (Invitrogen, Carlsbad, Calif.; catalog #31985088) with 1% FBS.

    Article Title: A Bispecific, Tetravalent Antibody Targeting Inflammatory and Pruritogenic Pathways in Atopic Dermatitis.
    Article Snippet: Inhibition curves were fitted using a 4-parameter logistic regression model in GraphPad prism 9.1.0.

    Incubation:

    Article Title: Compounds and methods for treatment of visceral pain
    Article Snippet: .. The plates were then incubated at 37° C./5% CO2 for two h. 12 μL of substrate prepared from the PathHunter Flash Detection Kit (DiscoveRx, Fremont, CA: catalog #93-0001) was transferred to each well of the Optiplate. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti-CCR7 antibody drug conjugates
    Article Snippet: .. TABLE 13 pH Dependency of Humanized Anti-CCR7 Antibodies to CCR7-Expressing VLPs ELISA; Kd (nM) Humanized CysMab pH 7.4 pH 5.8 fold change antibody buffer buffer (pH 5.8/7.4) 121G12 0.1411 0.5207 4 506E15 0.0571 0.4942 9 674J13 0.0162 0.1657 10 684E12 0.0374 0.5041 13 bArrestin Assay To determine functionality of the anti-CCR7 antibodies, the β-Arrestin assay was performed using the PathHunter Flash Detection Kit from DiscoverX (#93-0247) either in agonistic mode for assessment of agonistic function or antagonistic mode for assessment of antagonistic function. .. In the agonistic mode, CHO-flpin-hCCR7 (cell line made by DiscoverX expressing hCCR7 tagged with ProLink, β-arrestin-EA) were seeded at 8×104 cell/well in 20 μl/well in growth medium (Ham's F-12/Glutamax medium; Invitrogen+10% FBS+0.5 mg/ml G418+0.2 mg/ml hygromycinB; Invitrogen+5 μg/ml Blasticidin; Gibco) with Dox 100 ng/ml in 384-well plates, covered with metal lids, incubated at 37° C. 5% CO2 overnight.

    Beta-Arrestin Assay:

    Article Title: Anti-CCR7 antibody drug conjugates
    Article Snippet: .. TABLE 13 pH Dependency of Humanized Anti-CCR7 Antibodies to CCR7-Expressing VLPs ELISA; Kd (nM) Humanized CysMab pH 7.4 pH 5.8 fold change antibody buffer buffer (pH 5.8/7.4) 121G12 0.1411 0.5207 4 506E15 0.0571 0.4942 9 674J13 0.0162 0.1657 10 684E12 0.0374 0.5041 13 bArrestin Assay To determine functionality of the anti-CCR7 antibodies, the β-Arrestin assay was performed using the PathHunter Flash Detection Kit from DiscoverX (#93-0247) either in agonistic mode for assessment of agonistic function or antagonistic mode for assessment of antagonistic function. .. In the agonistic mode, CHO-flpin-hCCR7 (cell line made by DiscoverX expressing hCCR7 tagged with ProLink, β-arrestin-EA) were seeded at 8×104 cell/well in 20 μl/well in growth medium (Ham's F-12/Glutamax medium; Invitrogen+10% FBS+0.5 mg/ml G418+0.2 mg/ml hygromycinB; Invitrogen+5 μg/ml Blasticidin; Gibco) with Dox 100 ng/ml in 384-well plates, covered with metal lids, incubated at 37° C. 5% CO2 overnight.

    Binding Assay:

    Article Title: Crystalline forms and processes for the preparation of cannabinoid receptor modulators
    Article Snippet: .. Binding of β-arrestin to the activated GPCR causes the complementation of the two enzyme fragments, forming an active β-galactosidase enzyme which can be measured using the chemiluminescent PathHunter Flash Detection Kit (DiscoveRx, Fremont, Calif.: catalog #93-0001). .. The assay: The stable CHO-K1 cells expressing CB2—Prolink fusion protein were plated over night in 384-well plates (Optiplate 384-Plus, PerkinElmer, Fremont Calif.; catalog #6007299) at 5000 cells/5 μL/well in the Opti-MEM medium (Invitrogen, Carlsbad, Calif.; catalog #31985088) with 1% FBS.



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