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nst dapi buffer  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc nst dapi buffer
    Nst Dapi Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nst+dapi+buffer/DAPI/pmc11975464-787-5-60
    Average 98 stars, based on 2136 article reviews
    nst dapi buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Incubation:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Sections were then incubated with primary antibodies against UCP1 (Abcam, ab10983), PPARγ (Santa Cruz Biotechnology, sc-7273), and PDGFRα (R&D Systems, AF-307-NA) at 4 °C overnight. .. After washing, sections were incubated with appropriate fluorophore-conjugated secondary antibodies for 1 h at room temperature and counterstained with DAPI (Cell Signaling Technology, 4083). .. Images were acquired using a Zeiss LSM 800 confocal microscope.

    Article Title: Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics Approach.
    Article Snippet: .. The 300 μL blocking solution was added and blocked at 25 ◦C for 1 h. The slides were placed in primary antibodies, including monocarboxylate Transporter 1 [MCT1], MCT2 (PA5-72957, PA5-76603, Thermo Fisher Scientific, Pittsburgh, PA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), DAPI (4083, Cell Signaling Technology, Danvers, MA, USA), dilution ratio 1:200, and incubated at 4 ◦C overnight. .. The fluorescent secondary antibodies (Goat Anti-Mouse IgG H&L Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L Alexa Fluor® 647, dilution ratio 1:200, ab150113, ab150079, Abcam, Cambridge, UK) were added and incubated at 25 ◦C for 1 h. Using a fluorescence microscope (CKX53, Olympus Corporation, Tokyo, Japan), results were documented following the addition of anti-fade mounting medium, which was applied after a light-protected PBS wash.

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). ..

    Blocking Assay:

    Article Title: Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics Approach.
    Article Snippet: .. The 300 μL blocking solution was added and blocked at 25 ◦C for 1 h. The slides were placed in primary antibodies, including monocarboxylate Transporter 1 [MCT1], MCT2 (PA5-72957, PA5-76603, Thermo Fisher Scientific, Pittsburgh, PA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), DAPI (4083, Cell Signaling Technology, Danvers, MA, USA), dilution ratio 1:200, and incubated at 4 ◦C overnight. .. The fluorescent secondary antibodies (Goat Anti-Mouse IgG H&L Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L Alexa Fluor® 647, dilution ratio 1:200, ab150113, ab150079, Abcam, Cambridge, UK) were added and incubated at 25 ◦C for 1 h. Using a fluorescence microscope (CKX53, Olympus Corporation, Tokyo, Japan), results were documented following the addition of anti-fade mounting medium, which was applied after a light-protected PBS wash.

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). ..

    Staining:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. ..

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. .. After staining, cells were washed three times with PBS and imaged using an EVOS imaging system (Thermo Fisher).



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