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reducing or nonreducing loading buffer (Beyotime)

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Structured Review

Beyotime reducing or nonreducing loading buffer
Reducing Or Nonreducing Loading Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonreducing-loading+buffer/non+reducing+loading+buffer/pmc09433816-52-4-6
Average 90 stars, based on 1 article reviews

reducing or nonreducing loading buffer - by Bioz Stars, 2026-09

90/100 stars

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Concentration Assay:

Article Title: Effects of Heating Treatment on Functional and Structural Properties of Liquid Whole Egg.
Article Snippet: .. A total of 40 μL of the protein solution (2 mg/mL) was added to 10 μL of the reducing loading buffer (5 X) (Beyotime, Shanghai, China), and the other group was added to a non-reducing loading buffer (5 X) and heated in a boiling water bath for 5 min. A total of 10 μL of the protein solution was loaded on the gels with a thickness of 1 mm composed of 12% separation gel concentration and 5% concentration gel and run at 120 mV. .. Molecular mass standards of 10.0–180.0 kDa (Biosharp, Beijing, China) were used to estimate the molecular mass of samples.

Clear Native PAGE:

Article Title: Effects of Protein on Green Tea Quality in a Milk-Tea Model during Heat Treatment: Antioxidant Activity, Foaming Properties, and Unbound Small-molecule Metabolome.
Article Snippet: The samples were mixed with the loading buffer (Beyotime, China) and heated at 90°C for 5 min to obtain reduced SDS-PAGE samples. .. Similarly, the samples were mixed with non-reducing sample buffer (Beyotime, China) to obtain non-reducing Native-PAGE samples. .. Gels were prepared using a gel kit (Beyotime, Shanghai, China), and a standard protein molecular weight marker (Proteintech, America) was included.

other:

Article Title: Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking
Article Snippet: The lysis buffer used for Western blotting was non-reducing buffer (Beyotime, China); the sample buffer was reducing buffer (Beyotime, China).

Article Title: Impact of calcium ions at physiological concentrations on the adsorption behavior of proteins on silica nanoparticles.
Article Snippet: A R T I C L E I N F O

SDS Page:

Article Title: Inhibition of peptidyl-prolyl cis-trans isomerase B mediates cyclosporin A-induced apoptosis of islet β cells
Article Snippet: .. Reducing loading buffer or non-reducing loading buffer (lacked DTT and 2-mercaptoethanol; both Beyotime Institute of Biotechnology, Shanghai, China) was added to the supernatant, which was subsequently boiled for 5 min and then electrophoresed on a 12.5 or 10% SDS-PAGE gel. ..