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quickchange multisite mutagenesis kit  (Agilent technologies)


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    Structured Review

    Agilent technologies quickchange multisite mutagenesis kit
    Quickchange Multisite Mutagenesis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multisite-quickchange+kit/pmc03422271-281-9-13
    Average 90 stars, based on 1 article reviews
    quickchange multisite mutagenesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Mutagenesis:

    Article Title: Rodent Herpesvirus Peru Encodes a Secreted Chemokine Decoy Receptor
    Article Snippet: .. Mutagenesis was performed using a multisite QuickChange mutagenesis kit (Agilent Technologies) on the background of the wild-type R17 C-terminal His construct and verified by DNA sequencing. ..

    Article Title: Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression
    Article Snippet: The 3′-UTR of the Pax3 transcript was amplified by PCR by using forward 5′-AAAAGCGGCCGCGTTTAAACGAGAAGATTCTTCATGTCTA-3′ and reverse 5′-AAAACTCGAGGTTTAAACTAGGGGGTAGTTCCTCCT-3′ primers, cloned into the pGEMT-Easy vector (Promega), and the sequence was verified. .. The predicted miR-27b “seed” site was mutated with mutagenic primers 5′-GCTGATCTTGCCCCTAACCCCGAATTGTCCCAGCATGACC-3′ and 5′-GATCTTGCCCCTAACCCCCTTTTGTCCCAGCATGACC-3′ by using the QuickChange Multisite mutagenesis kit (Stratagene); mutations were confirmed by sequence analysis. .. The wild-type and mutated 3′-UTRs were subcloned into the NotI–XhoI site of the psicheck-2 vector (Promega).

    Article Title: Hmgb3 Is Regulated by MicroRNA-206 during Muscle Regeneration
    Article Snippet: .. The predicted miR seed sites were mutated using the QuickChange Multisite mutagenesis kit (Stratagene, Santa Clara, California, USA) with the following mutagenic primers:miR-206.1 F: 5′ GGCATAGCATTCTGCGGCCGGCATTAGTTGTGGAAAGG 3′miR-206.2 F: 5′ CCTAAACGCAATTTGCAGACGCATGTTATTTTTTGTATG 3′. ..

    Article Title: Dual-Color Imaging of Nuclear Division and Mitotic Spindle Elongation in Live Cells of Aspergillus nidulans
    Article Snippet: .. Three oligonucleotides (M1 [ACTTGTCACTACTCTCACCTGGGGTGTTCAATGC], M2 [ATACAACTACATCTCCCACAACGTATACATCACCGCCGACAAGCAG], and M3 [ACGGCATCAAAGCCCACTTCAAGACCCGC]) were used for mutagenesis with a Stratagene QuickChange Multisite mutagenesis kit. ..

    Construct:

    Article Title: Rodent Herpesvirus Peru Encodes a Secreted Chemokine Decoy Receptor
    Article Snippet: .. Mutagenesis was performed using a multisite QuickChange mutagenesis kit (Agilent Technologies) on the background of the wild-type R17 C-terminal His construct and verified by DNA sequencing. ..

    DNA Sequencing:

    Article Title: Rodent Herpesvirus Peru Encodes a Secreted Chemokine Decoy Receptor
    Article Snippet: .. Mutagenesis was performed using a multisite QuickChange mutagenesis kit (Agilent Technologies) on the background of the wild-type R17 C-terminal His construct and verified by DNA sequencing. ..

    Sequencing:

    Article Title: Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression
    Article Snippet: The 3′-UTR of the Pax3 transcript was amplified by PCR by using forward 5′-AAAAGCGGCCGCGTTTAAACGAGAAGATTCTTCATGTCTA-3′ and reverse 5′-AAAACTCGAGGTTTAAACTAGGGGGTAGTTCCTCCT-3′ primers, cloned into the pGEMT-Easy vector (Promega), and the sequence was verified. .. The predicted miR-27b “seed” site was mutated with mutagenic primers 5′-GCTGATCTTGCCCCTAACCCCGAATTGTCCCAGCATGACC-3′ and 5′-GATCTTGCCCCTAACCCCCTTTTGTCCCAGCATGACC-3′ by using the QuickChange Multisite mutagenesis kit (Stratagene); mutations were confirmed by sequence analysis. .. The wild-type and mutated 3′-UTRs were subcloned into the NotI–XhoI site of the psicheck-2 vector (Promega).

    other:

    Article Title: Clinical Evolution, Genetic Landscape, and Trajectories of Clonal Hematopoiesis in SAMD9/SAMD9L Syndromes
    Article Snippet: Here, we introduced patient-specific single (germline n=48, somatic n=7) and double ( cis germline/somatic n=6) SAMD9 or SAMD9L mutations into the vector with wildtype SAMD9 or SAMD9L cDNA by site-directed mutagenesis (QuickChange multisite-directed mutagenesis kit, Agilent Technologies).

    Article Title: The transcription factor GLI2 as a downstream mediator of transforming growth factor-β-induced fibroblast activation in SSc.
    Article Snippet: Department of Internal Medicine III and Institute for Clinical Immunology, University of Erlangen-Nuremberg, Erlangen, Germany Department of Clinical and Experimental Rheumatology, 1st Faculty of Medicine, Institute of Rheumatology, Charles University, Prague, Czech Republic Dipartimento Cardiotoracico e Vascolare, Laboratory of Respiratory Cell Biology, University of Pisa, Pisa, Italy Department of Dermatology, Venereology and Pediatric Dermatology, Medical University of Lublin, Poland Rheumaklinik, University Hospital Zurich, Zurich, Switzerland

    Article Title: Stereotyped antibody responses target posttranslationally modified gluten in celiac disease
    Article Snippet: Site-directed mutagenesis in 1E01 and 1E03 hmAbs was induced using Quickchange Multisite-Directed Mutagenesis Kit (Agilent) following the manufacture’s instructions.



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