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miscript primer detection kit  (Qiagen)


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    Qiagen miscript primer detection kit
    Miscript Primer Detection Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+primer+detection+kit/miscript+ii+rt+kit/pm38063121-48-33-37
    Average 90 stars, based on 1 article reviews
    miscript primer detection kit - by Bioz Stars, 2026-11
    90/100 stars

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    Generated:

    Article Title: miR-769-5p has diagnostic value in acute kidney injury in intensive care unit patients and mediates disease development by targeting SIRT6.
    Article Snippet: .. For miRNAs such as miR-769-5p, cDNA was generated with the use of miScript II RT Kit (Qiagen, Germany). .. Reverse transcription was performed for mRNA of SIRT6, renal tubular injury markers kidney injury molecule-1 (KIM-1), and Neutrophil gelatinase-associated lipocalin (NGAL) using SuperScriptTM IV Reverse Transcriptase Kit (Invitrogen, USA). miR-769-5p were amplified using miScript SYBR Green PCR Kit (Qiagen, Germany).

    Synthesized:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    SYBR Green Assay:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the miScript SYBR Green qPCR Kit (cat. no. 218076; Qiagen, Inc.) was used in a StepOne Real-Time PCR thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Polymerase Chain Reaction:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Sequencing:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the miScript SYBR Green qPCR Kit (cat. no. 218076; Qiagen, Inc.) was used in a StepOne Real-Time PCR thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Reverse Transcription:

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: As a negative control, cells were transfected with a scrambled sequence (Sc; cat. no. SC-37007; Santa Cruz Biotechnology, Inc.) at a final concentration of 10 nM under the aforementioned conditions. .. The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the miScript SYBR Green qPCR Kit (cat. no. 218076; Qiagen, Inc.) was used in a StepOne Real-Time PCR thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    cDNA Synthesis:

    Article Title: Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma
    Article Snippet: RNA integrity and concentration were evaluated with a NanoDrop ND-1000 spectrophotometer, measuring absorbance at 260/280 nm. .. Complementary DNA (cDNA) synthesis was carried out with 1 μg of miRNA using the miScript II RT Kit (Qiagen GmbH, Germany), adhering to the manufacturer's instructions. .. Complementary DNA (cDNA) synthesis was carried out with 1 μg of miRNA using the miScript II RT Kit (Qiagen GmbH, Germany), adhering to the manufacturer's instructions.



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    miscript primer detection kit  (Qiagen)
    90
    Qiagen miscript primer detection kit
    Miscript Primer Detection Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+primer+detection+kit/miscript+ii+rt+kit/pm38063121-48-33-37
    Average 90 stars, based on 1 article reviews
    miscript primer detection kit - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

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