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microarray‐based gene expression analysis guide (v.6.5)  (Agilent technologies)


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    Agilent technologies microarray‐based gene expression analysis guide (v.6.5)
    Microarray‐Based Gene Expression Analysis Guide (V.6.5), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray-based+gene+expression+analysis+guide/pmc08251598-98-13-12
    Average 90 stars, based on 1 article reviews
    microarray‐based gene expression analysis guide (v.6.5) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis
    Article Snippet: Five hundred nanograms of total RNA from each treated samples, were separately reverse-transcribed into double-strand cDNAs by the Moloney murine leukemia virus reverse transcriptase (MMLV-RT) and amplified for 2 h at 40°C using the Agilent Quick Amp Labelling Kit, two-color (Agilent Technologies).

    Article Title: Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis
    Article Snippet: Oct4-EGFP-high cells and Oct4-EGFP-negative cells were sorted using an SH800 cell sorter (SONY), and total RNA was prepared using an RNA Purification Kit (Qiagen).

    Gene Expression:

    Article Title: Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells
    Article Snippet: Agilent RNA ScreenTape System (Agilent Technologies, Santa Clara, CA, United States) was used to evaluate the RNA integrity ( ). .. A huge panel of genes of interest (maximum 3,000 genes, including at least five replicates for each gene) was selected and analyzed through a custom gene expression microarray designed through Agilent Technologies ( https://earray.chem.agilent.com/earray/ , date of access 12 February 2018) ( ; ; ). ..

    Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum
    Article Snippet: .. Global gene expression analysis using the Agilent gene expression microarray revealed the expression of 3926 genes that were changed by acid and Al ions, in which 66.33% can be detected in root including those specifically expressing in root which accounted for 29.98% of all the genes. .. Global gene expression analysis using the Agilent gene expression microarray revealed the expression of 3926 genes that were changed by acid and Al ions, in which 66.33% can be detected in root including those specifically expressing in root which accounted for 29.98% of all the genes.

    Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD
    Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Agilent gene expression microarray and Affymetrix SNP chip. ..

    Article Title: Lymphatic endothelial sphingosine 1-phosphate receptor 1 enhances macrophage clearance via lymphatic system following myocardial infarction
    Article Snippet: Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0). .. Total RNA from hearts or LECs was extracted by Trizol (Invitrogen) following the manufacturer’s instructions and used for RT-PCR (the primer list was listed in ) or high-throughput Gene Expression microarray (Agilent Technology). .. RNA quantity and quality were measured by NanoDrop ND-1000.

    Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum
    Article Snippet: .. Twelve genes encoding peroxidase (GO:0004601) could eliminate ROS production, leading to the plant morphological changes induced by Al. A total of 20 genes encoding transporters were found in our Agilent gene expression microarray analysis, in which 8 genes encoded ABC transporters. .. Twelve genes encoding peroxidase (GO:0004601) could eliminate ROS production, leading to the plant morphological changes induced by Al. A total of 20 genes encoding transporters were found in our Agilent gene expression microarray analysis, in which 8 genes encoded ABC transporters.

    Article Title: Commensal Microbiota Contributes to Chronic Endocarditis in TAX1BP1 Deficient Mice
    Article Snippet: .. Then, global mRNA expression profiles were analyzed by an Agilent gene expression microarray. ..

    Microarray:

    Article Title: Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells
    Article Snippet: Agilent RNA ScreenTape System (Agilent Technologies, Santa Clara, CA, United States) was used to evaluate the RNA integrity ( ). .. A huge panel of genes of interest (maximum 3,000 genes, including at least five replicates for each gene) was selected and analyzed through a custom gene expression microarray designed through Agilent Technologies ( https://earray.chem.agilent.com/earray/ , date of access 12 February 2018) ( ; ; ). ..

    Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum
    Article Snippet: .. Global gene expression analysis using the Agilent gene expression microarray revealed the expression of 3926 genes that were changed by acid and Al ions, in which 66.33% can be detected in root including those specifically expressing in root which accounted for 29.98% of all the genes. .. Global gene expression analysis using the Agilent gene expression microarray revealed the expression of 3926 genes that were changed by acid and Al ions, in which 66.33% can be detected in root including those specifically expressing in root which accounted for 29.98% of all the genes.

    Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD
    Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Agilent gene expression microarray and Affymetrix SNP chip. ..

    Article Title: Lymphatic endothelial sphingosine 1-phosphate receptor 1 enhances macrophage clearance via lymphatic system following myocardial infarction
    Article Snippet: Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0). .. Total RNA from hearts or LECs was extracted by Trizol (Invitrogen) following the manufacturer’s instructions and used for RT-PCR (the primer list was listed in ) or high-throughput Gene Expression microarray (Agilent Technology). .. RNA quantity and quality were measured by NanoDrop ND-1000.

    Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum
    Article Snippet: .. Twelve genes encoding peroxidase (GO:0004601) could eliminate ROS production, leading to the plant morphological changes induced by Al. A total of 20 genes encoding transporters were found in our Agilent gene expression microarray analysis, in which 8 genes encoded ABC transporters. .. Twelve genes encoding peroxidase (GO:0004601) could eliminate ROS production, leading to the plant morphological changes induced by Al. A total of 20 genes encoding transporters were found in our Agilent gene expression microarray analysis, in which 8 genes encoded ABC transporters.

    Article Title: Commensal Microbiota Contributes to Chronic Endocarditis in TAX1BP1 Deficient Mice
    Article Snippet: .. Then, global mRNA expression profiles were analyzed by an Agilent gene expression microarray. ..

    Expressing:

    Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum
    Article Snippet: .. Global gene expression analysis using the Agilent gene expression microarray revealed the expression of 3926 genes that were changed by acid and Al ions, in which 66.33% can be detected in root including those specifically expressing in root which accounted for 29.98% of all the genes. .. Global gene expression analysis using the Agilent gene expression microarray revealed the expression of 3926 genes that were changed by acid and Al ions, in which 66.33% can be detected in root including those specifically expressing in root which accounted for 29.98% of all the genes.

    Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD
    Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Agilent gene expression microarray and Affymetrix SNP chip. ..

    Article Title: Commensal Microbiota Contributes to Chronic Endocarditis in TAX1BP1 Deficient Mice
    Article Snippet: .. Then, global mRNA expression profiles were analyzed by an Agilent gene expression microarray. ..

    Sequencing:

    Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD
    Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Agilent gene expression microarray and Affymetrix SNP chip. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Lymphatic endothelial sphingosine 1-phosphate receptor 1 enhances macrophage clearance via lymphatic system following myocardial infarction
    Article Snippet: Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0). .. Total RNA from hearts or LECs was extracted by Trizol (Invitrogen) following the manufacturer’s instructions and used for RT-PCR (the primer list was listed in ) or high-throughput Gene Expression microarray (Agilent Technology). .. RNA quantity and quality were measured by NanoDrop ND-1000.



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    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by <t>microarray</t> analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.
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    Reverse transcription‐polymerase chain reaction ( RT‐PCR ) validation. Gene expression from sedentary nematodes expressed as the fold change referenced to J 2 in the stages <t> microarray </t> experiment, and gene expression from sedentary nematodes in partially resistant line 11315 expressed as the fold change referenced to cv. D esirée in the genotypes <t> microarray </t> experiment
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    Image Search Results


    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Journal: Journal of Experimental Botany

    Article Title: NAC61 regulates late- and post-ripening osmotic, oxidative, and biotic stress responses in grapevine

    doi: 10.1093/jxb/erad507

    Figure Lengend Snippet: NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Article Snippet: The cDNA synthesis, labelling, hybridization, and washing steps were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide ( https://www.agilent.com/cs/library/usermanuals/Public/G4140-90040_GeneExpression_OneColor_6.9.pdf ).

    Techniques: Expressing, Microarray

    Expression analyses in petunia petals by qPCR. (A) Expression analysis of F3 ′ H and N21 in the ph3 mutant and in VvWRKY26 expressing lines as confirmation of the microarray results. (B) Expression analysis of structural genes related to vacuolar acidification (PhPH5 and PhPH1) and to anthocyanin synthesis (PhCHS-A and PhDFR-A) in the untransformed R27 and ph3 lines and VvWRKY26 expressing plants. In all analyses the data correspond to the mean ± SE of three biological replicates (corresponding to lines 1, 2, and 5; Supplementary Figure ) relative to an ACTIN housekeeping control and normalized against the ph3 mutant value. Abbreviations correspond to: PhF3 ′ H, FLAVONOID-3 ′ -MONOOXYGENASE; PhN21, NODULIN MTN21-LIKE PROTEIN; PhPH5, H + P 3 A -ATPASE; PhPH1, P 3 B -ATPASE; PhCHS-A, CHALCONE SYNTHASE A; PhDFR-A, DIHYDROFLAVONOL 4-REDUCTASE A .

    Journal: Frontiers in Plant Science

    Article Title: A Grapevine TTG2-Like WRKY Transcription Factor Is Involved in Regulating Vacuolar Transport and Flavonoid Biosynthesis

    doi: 10.3389/fpls.2016.01979

    Figure Lengend Snippet: Expression analyses in petunia petals by qPCR. (A) Expression analysis of F3 ′ H and N21 in the ph3 mutant and in VvWRKY26 expressing lines as confirmation of the microarray results. (B) Expression analysis of structural genes related to vacuolar acidification (PhPH5 and PhPH1) and to anthocyanin synthesis (PhCHS-A and PhDFR-A) in the untransformed R27 and ph3 lines and VvWRKY26 expressing plants. In all analyses the data correspond to the mean ± SE of three biological replicates (corresponding to lines 1, 2, and 5; Supplementary Figure ) relative to an ACTIN housekeeping control and normalized against the ph3 mutant value. Abbreviations correspond to: PhF3 ′ H, FLAVONOID-3 ′ -MONOOXYGENASE; PhN21, NODULIN MTN21-LIKE PROTEIN; PhPH5, H + P 3 A -ATPASE; PhPH1, P 3 B -ATPASE; PhCHS-A, CHALCONE SYNTHASE A; PhDFR-A, DIHYDROFLAVONOL 4-REDUCTASE A .

    Article Snippet: For microarray analysis on Sultana leaves transiently over-expressing VvWRKY26 , the cDNA synthesis, labeling, hybridization and washing reactions were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).

    Techniques: Expressing, Mutagenesis, Microarray

    Reverse transcription‐polymerase chain reaction ( RT‐PCR ) validation. Gene expression from sedentary nematodes expressed as the fold change referenced to J 2 in the stages microarray experiment, and gene expression from sedentary nematodes in partially resistant line 11315 expressed as the fold change referenced to cv. D esirée in the genotypes microarray experiment

    Journal: Molecular Plant Pathology

    Article Title: Comparison of transcript profiles in different life stages of the nematode Globodera pallida under different host potato genotypes

    doi: 10.1111/j.1364-3703.2012.00821.x

    Figure Lengend Snippet: Reverse transcription‐polymerase chain reaction ( RT‐PCR ) validation. Gene expression from sedentary nematodes expressed as the fold change referenced to J 2 in the stages microarray experiment, and gene expression from sedentary nematodes in partially resistant line 11315 expressed as the fold change referenced to cv. D esirée in the genotypes microarray experiment

    Article Snippet: All microarray hybridization and washing procedures were performed according to the Two‐Color Microarray‐Based Gene Expression Analysis Guide (version 5.7; Agilent Technologies).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Microarray