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Genechem microarray probes
Microarray Probes, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+probes/microarray/pm40396326-92-6-15
Average 90 stars, based on 1 article reviews
microarray probes - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Hybridization:

Article Title: Apatinib inhibits glioma cell malignancy in patient-derived orthotopic xenograft mouse model by targeting thrombospondin 1/myosin heavy chain 9 axis
Article Snippet: The RNA was reverse transcribed to cDNA using the GeneChip WT PLUS Kit (Thermo Scientific), and the cDNA was purified, fragmented, and hybridized with the microarray probe. .. After hybridization, the microarray chip (Genechem) was washed and dyed, and images, as well as raw data, were obtained. ..

Article Title: Inhibition of Sox4 Increases the Sensitivity of Drug-resistant Melanoma Cells to Cisplatin through the P38 Signaling Pathway.
Article Snippet: Background: SRY-Box Transcription Factor 4 (Sox4) has been found to be overexpressed in a number of malignancies and is linked to medication resistance.. The underlying mechanism of Sox4 in cisplatin-resistant melanomas, however, remains unknown.. Methods: Immunohistochemistry (IHC) was used to examine the expression of Sox4 in melanoma, pigmented nevi, and normal skin tissue.

Microarray:

Article Title: Apatinib inhibits glioma cell malignancy in patient-derived orthotopic xenograft mouse model by targeting thrombospondin 1/myosin heavy chain 9 axis
Article Snippet: The RNA was reverse transcribed to cDNA using the GeneChip WT PLUS Kit (Thermo Scientific), and the cDNA was purified, fragmented, and hybridized with the microarray probe. .. After hybridization, the microarray chip (Genechem) was washed and dyed, and images, as well as raw data, were obtained. ..

Article Title: CSE1L in enhancing the effect of defactinib on gastric cancer cells via the inhibition of FAK phosphorylation
Article Snippet: .. Posthybridization, a microarray chip (GeneChem, Shanghai, China) was processed by washing and staining. ..

Article Title: Inhibition of Sox4 Increases the Sensitivity of Drug-resistant Melanoma Cells to Cisplatin through the P38 Signaling Pathway.
Article Snippet: Background: SRY-Box Transcription Factor 4 (Sox4) has been found to be overexpressed in a number of malignancies and is linked to medication resistance.. The underlying mechanism of Sox4 in cisplatin-resistant melanomas, however, remains unknown.. Methods: Immunohistochemistry (IHC) was used to examine the expression of Sox4 in melanoma, pigmented nevi, and normal skin tissue.

Article Title: Modification of Mesenchymal Stem Cells by HMGB1 Promotes the Activity of Cav3.2 T-type Calcium Channel via PKA/β-catenin/γ-cystathionase Pathway
Article Snippet: .. RNA microarray Microarray analysis of gene expression was performed by Genechem (Shanghai, China). .. Brie y, total RNA was extracted from MSC-H and MSC-C cells by using RNAeasyTM plus animal RNA isolation kit with spin column (Beyotime, China).

Article Title: FGF21 protects against doxorubicin-induced cardiotoxicity by inhibiting connexin 43 ubiquitination.
Article Snippet: Background: Fibroblast growth factor 21 (FGF21) regulates glycolipid metabolism and insulin homeostasis and acts as a cardioprotective factor by protecting against myocardial ischemia/reperfusion injury, hypertension, and vascular dysfunction.. FGF21 has been reported to prevent Doxorubicin (Dox)-induced cardiotoxicity, and the related signaling pathway is worthy of further study.. Connexin43 (Cx43) protein was reduced by Dox treatment, especially low phosphorylated form of Cx43.

Staining:

Article Title: CSE1L in enhancing the effect of defactinib on gastric cancer cells via the inhibition of FAK phosphorylation
Article Snippet: .. Posthybridization, a microarray chip (GeneChem, Shanghai, China) was processed by washing and staining. ..

Gene Expression:

Article Title: Modification of Mesenchymal Stem Cells by HMGB1 Promotes the Activity of Cav3.2 T-type Calcium Channel via PKA/β-catenin/γ-cystathionase Pathway
Article Snippet: .. RNA microarray Microarray analysis of gene expression was performed by Genechem (Shanghai, China). .. Brie y, total RNA was extracted from MSC-H and MSC-C cells by using RNAeasyTM plus animal RNA isolation kit with spin column (Beyotime, China).

other:

Article Title: MTHFD1L confers a poor prognosis and malignant phenotype in esophageal squamous cell carcinoma by activating the ERK5 signaling pathway.
Article Snippet: MTHFD1L, a key enzyme of folate metabolism, is seldom reported in cancer.. In this study, we investigate the role of MTHFD1L in the tumorigenicity of esophageal squamous cell carcinoma (ESCC).. ESCC tissue microarrays (TMAs) containing 177 samples from 109 patients were utilized to evaluate whether MTHFD1L expression, determined using immunohistochemical analysis, is a prognostic indicator for ESCC patients.

Article Title: GDNF Promotes Astrocyte Abnormal Proliferation and Migration Through the GFRα1/RET/MAPK/pCREB/LOXL2 Signaling Axis.
Article Snippet: Glial cell-line derived neurotrophic factor (GDNF) is a powerful astroglioma (AG) proliferation and migration factor that is highly expressed in AG cells derived from astrocytes.. However, it is still unclear whether high levels of GDNF promote AG occurrence or if they are secondary to AG formation.. We previously reported that high concentrations of GDNF (200 and 500 ng/mL) can inhibit DNA damage-induced rat primary astrocytes (RA) apoptosis, suggesting that high concentrations of GDNF may be involved in the malignant transformation of astrocytes to AG cells.

Article Title: The polysaccharide-peptide complex from mushroom Cordyceps militaris ameliorates atherosclerosis by modulating the lncRNA-miRNA-mRNA axis.
Article Snippet: An N-glycosidic polysaccharide–peptide complex CMPS-80 was obtained from the fruiting body of C. militaris.. Of importance, CMPS-80 significantly ameliorated formation of atherosclerotic lesions and plasma lipid profiles in apolipoprotein E-deficient mice.. Integrated informatics analysis suggested that CMPS-80 can modulate multiple lncRNA–microRNA–mRNA axes.

Expressing:

Article Title: FGF21 protects against doxorubicin-induced cardiotoxicity by inhibiting connexin 43 ubiquitination.
Article Snippet: Background: Fibroblast growth factor 21 (FGF21) regulates glycolipid metabolism and insulin homeostasis and acts as a cardioprotective factor by protecting against myocardial ischemia/reperfusion injury, hypertension, and vascular dysfunction.. FGF21 has been reported to prevent Doxorubicin (Dox)-induced cardiotoxicity, and the related signaling pathway is worthy of further study.. Connexin43 (Cx43) protein was reduced by Dox treatment, especially low phosphorylated form of Cx43.



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We collected published datasets of human blood samples for gene expression, DNA methylation, and single-cell transcriptomic data. The analysis aimed to study the relation between the expression and DNA methylation of retrotransposons (RTEs) versus chronological and biological aging in large human cohorts. The single-cell transcriptomic datasets were employed for cell type-specific analysis of RTEs in peripheral blood mononuclear cell (PBMC) to identify the relation between RTE expression and aging events for annotated cell types within old versus young PBMC samples.

Journal: eLife

Article Title: Expression of most retrotransposons in human blood correlates with biological aging

doi: 10.7554/eLife.96575

Figure Lengend Snippet: We collected published datasets of human blood samples for gene expression, DNA methylation, and single-cell transcriptomic data. The analysis aimed to study the relation between the expression and DNA methylation of retrotransposons (RTEs) versus chronological and biological aging in large human cohorts. The single-cell transcriptomic datasets were employed for cell type-specific analysis of RTEs in peripheral blood mononuclear cell (PBMC) to identify the relation between RTE expression and aging events for annotated cell types within old versus young PBMC samples.

Article Snippet: By overlapping Illumina microarray expression and methylation probe locations to RTE locations in RepeatMasker , we were able to identify a sufficient number of probes to calculate the expression and methylation levels of RTE classes and families.

Techniques: Gene Expression, DNA Methylation Assay, Expressing

(a, b), Methylation levels of RTE classes inversely correlated with chronological age in monocyte (Multi-Ethnic Study of Atherosclerosis, MESA) and whole blood (WB) (BSGS, SATSA, and GMPWAR) samples. Satellite DNA was included as a control group. ( c ), Methylation levels versus low (first quartile), medium (second and third quartile), and high (fourth quartile) expressions of RTE classes in monocytes (MESA). Wilcoxon test; ns: not significant. ( d ), Correlation matrix for RTE expressions and methylation levels, and chronological age. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, Pearson’s correlation. MESA, n=1202; BSGS, n=614; GMPWAR, n=656; SATSA, n=1072.

Journal: eLife

Article Title: Expression of most retrotransposons in human blood correlates with biological aging

doi: 10.7554/eLife.96575

Figure Lengend Snippet: (a, b), Methylation levels of RTE classes inversely correlated with chronological age in monocyte (Multi-Ethnic Study of Atherosclerosis, MESA) and whole blood (WB) (BSGS, SATSA, and GMPWAR) samples. Satellite DNA was included as a control group. ( c ), Methylation levels versus low (first quartile), medium (second and third quartile), and high (fourth quartile) expressions of RTE classes in monocytes (MESA). Wilcoxon test; ns: not significant. ( d ), Correlation matrix for RTE expressions and methylation levels, and chronological age. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, Pearson’s correlation. MESA, n=1202; BSGS, n=614; GMPWAR, n=656; SATSA, n=1072.

Article Snippet: By overlapping Illumina microarray expression and methylation probe locations to RTE locations in RepeatMasker , we were able to identify a sufficient number of probes to calculate the expression and methylation levels of RTE classes and families.

Techniques: Methylation, Control

(a, b), Methylation levels of long terminal repeats (LTR) and LINE/SINE families negatively correlate with chronological age in monocytes (MESA) and the WB (BSGS, SATSA, and GMPWAR). **p≤0.01, ***p≤0.001, Wilcoxon test.

Journal: eLife

Article Title: Expression of most retrotransposons in human blood correlates with biological aging

doi: 10.7554/eLife.96575

Figure Lengend Snippet: (a, b), Methylation levels of long terminal repeats (LTR) and LINE/SINE families negatively correlate with chronological age in monocytes (MESA) and the WB (BSGS, SATSA, and GMPWAR). **p≤0.01, ***p≤0.001, Wilcoxon test.

Article Snippet: By overlapping Illumina microarray expression and methylation probe locations to RTE locations in RepeatMasker , we were able to identify a sufficient number of probes to calculate the expression and methylation levels of RTE classes and families.

Techniques: Methylation

While LINE families, MIR, and long terminal repeats (LTR) families except ERVK show lower levels of methylation in higher expression groups, this pattern is not seen in Alu, CR1, and ERVl. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, ns: not significant, Wilcoxon test.

Journal: eLife

Article Title: Expression of most retrotransposons in human blood correlates with biological aging

doi: 10.7554/eLife.96575

Figure Lengend Snippet: While LINE families, MIR, and long terminal repeats (LTR) families except ERVK show lower levels of methylation in higher expression groups, this pattern is not seen in Alu, CR1, and ERVl. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, ns: not significant, Wilcoxon test.

Article Snippet: By overlapping Illumina microarray expression and methylation probe locations to RTE locations in RepeatMasker , we were able to identify a sufficient number of probes to calculate the expression and methylation levels of RTE classes and families.

Techniques: Methylation, Expressing

Set Enrichment Analysis (GSEA) of A549 Cell Line Treated with 5-Aza. The running ES indicates the degree to which this gene set is overrepresented at the top or bottom of the entire ranked list of genes. GSEA plots are a ranked list of genes from microarray analysis, sorted by their correlation with treatment effect. The color code bar (red to blue) represents the correlation of gene expression with the phenotype of interest, with red indicating a positive correlation and blue indicating a negative correlation. The leading-edge subset of genes, those contributing most to the ES, are highlighted within this list.

Journal: Medicine and Pharmacy Reports

Article Title: 5-Azacytidine treatment inhibits the development of lung cancer models via epigenetic reprogramming and activation of cellular pathways with anti-tumor activity

doi: 10.15386/mpr-2777

Figure Lengend Snippet: Set Enrichment Analysis (GSEA) of A549 Cell Line Treated with 5-Aza. The running ES indicates the degree to which this gene set is overrepresented at the top or bottom of the entire ranked list of genes. GSEA plots are a ranked list of genes from microarray analysis, sorted by their correlation with treatment effect. The color code bar (red to blue) represents the correlation of gene expression with the phenotype of interest, with red indicating a positive correlation and blue indicating a negative correlation. The leading-edge subset of genes, those contributing most to the ES, are highlighted within this list.

Article Snippet: Prior to hybridizatiion, the microarray probes (cRNA-Cy3) underwent purification using the RNeasy Mini Kit (Qiagen, Germany).

Techniques: Microarray, Gene Expression

Comparative analysis of gene expression changes from  microarray  and qPCR analysis.

Journal: Medicine and Pharmacy Reports

Article Title: 5-Azacytidine treatment inhibits the development of lung cancer models via epigenetic reprogramming and activation of cellular pathways with anti-tumor activity

doi: 10.15386/mpr-2777

Figure Lengend Snippet: Comparative analysis of gene expression changes from microarray and qPCR analysis.

Article Snippet: Prior to hybridizatiion, the microarray probes (cRNA-Cy3) underwent purification using the RNeasy Mini Kit (Qiagen, Germany).

Techniques: Gene Expression, Microarray, Biomarker Discovery