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whole human genome oligo microarray 4×44 k probe set  (Agilent technologies)


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    Agilent technologies whole human genome oligo microarray 4×44 k probe set
    Whole Human Genome Oligo Microarray 4×44 K Probe Set, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+probe+sets/us11535825-334-17-16
    Average 90 stars, based on 1 article reviews
    whole human genome oligo microarray 4×44 k probe set - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Adult stem cell compositions and methods of identification and isolation
    Article Snippet: Cy3- and Cy5-labeled cDNAs were combined and hybridized for 17 hours at 65° C. to the Agilent Whole Human Genome Oligo Microarray 4×44 K probe set using Agilent's recommended hybridization chamber and oven.

    Article Title: Anisomycin has the potential to induce human ovarian cancer stem cell ferroptosis by influencing glutathione metabolism and autophagy signal transduction pathways
    Article Snippet: The fluorescent images of the hybridized microarray were scanned using an Agilent Whole Human Genome 4×44 microarray scanner system.

    Article Title: Systems medicine platform for personalized oncology
    Article Snippet: Agilent 4×44 chips contain a set of non-control probes that are replicated up to ten times.

    Microarray:

    Article Title: A prognostic Risk Score model for oral squamous cell carcinoma constructed by 6 glycolysis-immune-related genes.
    Article Snippet: .. The data of OSCC patients were obtained by the Agilent-014850 Whole Human Genome Microarray 4×44 K G4112F, Illumina HumanHT-12 V4.0 expression beadchip and Affymetrix Human Genome U133 Plus 2.0 Array. ..

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As comparison controls, human normal fibroblast cells, human ES cells and standard human iPS cell strain 201B7 (provided by Dr. Shinya Yamanaka, Kyoto University; as prepared by introducing hOct4, hSox2, hKlf4 and hc-Myc genes with a retrovirus vector) were cultured and RNA extracted in the same manner. (2) Analysis of Gene Expression Total cellular RNA (0.5 μg) was labeled with Cy3 using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized to Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.) using Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and signals were obtained with an Agilent DNA microarray scanner. .. The thus obtained signals were analyzed with GeneSpringGX10 software (Agilent Technologies, Inc.), and gene expressions in individual cell clones were analyzed by a display method called Heat Map in which the intensity of gene expression is indicated by a gradient from red to green (FIG. 38).

    Article Title: Gene expression confirms a potentially receptive endometrium identified by histology in fertile women.
    Article Snippet: .. Linear polymerase chain reaction (PCR) was performed to increase the mRNA abundance for microarrays (NuGENTechnologies1, 2008). mRNA expression was measured (Caretti et al., 2008) using Affymetrix U133 + 2.0 microarrays (Affymetrix, 2003) with ow nloaded from https://academ ic.oup.com /hum rep/article/27/9/2747/627856 by guest on 16 June 2024 independent validation by a second Agilent 4×44 microarray platform (AgilentTechnologies, 2009) on a subset of samples. .. For data analysis of array platforms hybridized with mRNA isolated from the stromal and glandular epithelium, the Affymetrix raw data were background corrected, quantile normalized and summarized using the RMA method from the Affymetrix package from Bioconductor (Gautier et al., 2004) and Agilent data were quantile normalized.

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As a control, five human ES cell lines established at the Institute for Frontier Medical Sciences, Kyoto University, were cultured in the absence of feeder cells, and whole cell RNA was extracted, in the same manner. (2) Analysis of Gene Expression 0.5 μg of whole cell RNA was labeled with Cy3, using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized with Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.), using a Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and a signal was acquired using Agilent DNA Microarray Scanner. .. The acquired signal was analyzed using GeneSpringGX10 software (Agilent Technologies, Inc.) to obtain a correlation coefficient between respective gene expression patterns of cell clones (FIG. 27A).

    Expressing:

    Article Title: A prognostic Risk Score model for oral squamous cell carcinoma constructed by 6 glycolysis-immune-related genes.
    Article Snippet: .. The data of OSCC patients were obtained by the Agilent-014850 Whole Human Genome Microarray 4×44 K G4112F, Illumina HumanHT-12 V4.0 expression beadchip and Affymetrix Human Genome U133 Plus 2.0 Array. ..

    Article Title: Gene expression confirms a potentially receptive endometrium identified by histology in fertile women.
    Article Snippet: .. Linear polymerase chain reaction (PCR) was performed to increase the mRNA abundance for microarrays (NuGENTechnologies1, 2008). mRNA expression was measured (Caretti et al., 2008) using Affymetrix U133 + 2.0 microarrays (Affymetrix, 2003) with ow nloaded from https://academ ic.oup.com /hum rep/article/27/9/2747/627856 by guest on 16 June 2024 independent validation by a second Agilent 4×44 microarray platform (AgilentTechnologies, 2009) on a subset of samples. .. For data analysis of array platforms hybridized with mRNA isolated from the stromal and glandular epithelium, the Affymetrix raw data were background corrected, quantile normalized and summarized using the RMA method from the Affymetrix package from Bioconductor (Gautier et al., 2004) and Agilent data were quantile normalized.

    Labeling:

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As comparison controls, human normal fibroblast cells, human ES cells and standard human iPS cell strain 201B7 (provided by Dr. Shinya Yamanaka, Kyoto University; as prepared by introducing hOct4, hSox2, hKlf4 and hc-Myc genes with a retrovirus vector) were cultured and RNA extracted in the same manner. (2) Analysis of Gene Expression Total cellular RNA (0.5 μg) was labeled with Cy3 using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized to Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.) using Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and signals were obtained with an Agilent DNA microarray scanner. .. The thus obtained signals were analyzed with GeneSpringGX10 software (Agilent Technologies, Inc.), and gene expressions in individual cell clones were analyzed by a display method called Heat Map in which the intensity of gene expression is indicated by a gradient from red to green (FIG. 38).

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As a control, five human ES cell lines established at the Institute for Frontier Medical Sciences, Kyoto University, were cultured in the absence of feeder cells, and whole cell RNA was extracted, in the same manner. (2) Analysis of Gene Expression 0.5 μg of whole cell RNA was labeled with Cy3, using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized with Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.), using a Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and a signal was acquired using Agilent DNA Microarray Scanner. .. The acquired signal was analyzed using GeneSpringGX10 software (Agilent Technologies, Inc.) to obtain a correlation coefficient between respective gene expression patterns of cell clones (FIG. 27A).

    DNA Array:

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As comparison controls, human normal fibroblast cells, human ES cells and standard human iPS cell strain 201B7 (provided by Dr. Shinya Yamanaka, Kyoto University; as prepared by introducing hOct4, hSox2, hKlf4 and hc-Myc genes with a retrovirus vector) were cultured and RNA extracted in the same manner. (2) Analysis of Gene Expression Total cellular RNA (0.5 μg) was labeled with Cy3 using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized to Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.) using Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and signals were obtained with an Agilent DNA microarray scanner. .. The thus obtained signals were analyzed with GeneSpringGX10 software (Agilent Technologies, Inc.), and gene expressions in individual cell clones were analyzed by a display method called Heat Map in which the intensity of gene expression is indicated by a gradient from red to green (FIG. 38).

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As a control, five human ES cell lines established at the Institute for Frontier Medical Sciences, Kyoto University, were cultured in the absence of feeder cells, and whole cell RNA was extracted, in the same manner. (2) Analysis of Gene Expression 0.5 μg of whole cell RNA was labeled with Cy3, using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized with Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.), using a Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and a signal was acquired using Agilent DNA Microarray Scanner. .. The acquired signal was analyzed using GeneSpringGX10 software (Agilent Technologies, Inc.) to obtain a correlation coefficient between respective gene expression patterns of cell clones (FIG. 27A).

    Gene Expression:

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As comparison controls, human normal fibroblast cells, human ES cells and standard human iPS cell strain 201B7 (provided by Dr. Shinya Yamanaka, Kyoto University; as prepared by introducing hOct4, hSox2, hKlf4 and hc-Myc genes with a retrovirus vector) were cultured and RNA extracted in the same manner. (2) Analysis of Gene Expression Total cellular RNA (0.5 μg) was labeled with Cy3 using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized to Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.) using Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and signals were obtained with an Agilent DNA microarray scanner. .. The thus obtained signals were analyzed with GeneSpringGX10 software (Agilent Technologies, Inc.), and gene expressions in individual cell clones were analyzed by a display method called Heat Map in which the intensity of gene expression is indicated by a gradient from red to green (FIG. 38).

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As a control, five human ES cell lines established at the Institute for Frontier Medical Sciences, Kyoto University, were cultured in the absence of feeder cells, and whole cell RNA was extracted, in the same manner. (2) Analysis of Gene Expression 0.5 μg of whole cell RNA was labeled with Cy3, using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized with Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.), using a Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and a signal was acquired using Agilent DNA Microarray Scanner. .. The acquired signal was analyzed using GeneSpringGX10 software (Agilent Technologies, Inc.) to obtain a correlation coefficient between respective gene expression patterns of cell clones (FIG. 27A).

    Hybridization:

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As comparison controls, human normal fibroblast cells, human ES cells and standard human iPS cell strain 201B7 (provided by Dr. Shinya Yamanaka, Kyoto University; as prepared by introducing hOct4, hSox2, hKlf4 and hc-Myc genes with a retrovirus vector) were cultured and RNA extracted in the same manner. (2) Analysis of Gene Expression Total cellular RNA (0.5 μg) was labeled with Cy3 using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized to Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.) using Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and signals were obtained with an Agilent DNA microarray scanner. .. The thus obtained signals were analyzed with GeneSpringGX10 software (Agilent Technologies, Inc.), and gene expressions in individual cell clones were analyzed by a display method called Heat Map in which the intensity of gene expression is indicated by a gradient from red to green (FIG. 38).

    Article Title: Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
    Article Snippet: As a control, five human ES cell lines established at the Institute for Frontier Medical Sciences, Kyoto University, were cultured in the absence of feeder cells, and whole cell RNA was extracted, in the same manner. (2) Analysis of Gene Expression 0.5 μg of whole cell RNA was labeled with Cy3, using Quick Amp Labeling Kit (Agilent Technologies, Inc.). .. The labeled RNA was hybridized with Whole Human Genome (4×44 k) DNA array (Agilent Technologies, Inc.), using a Gene Expression Hybridization Kit (Agilent Technologies, Inc.), and a signal was acquired using Agilent DNA Microarray Scanner. .. The acquired signal was analyzed using GeneSpringGX10 software (Agilent Technologies, Inc.) to obtain a correlation coefficient between respective gene expression patterns of cell clones (FIG. 27A).

    Polymerase Chain Reaction:

    Article Title: Gene expression confirms a potentially receptive endometrium identified by histology in fertile women.
    Article Snippet: .. Linear polymerase chain reaction (PCR) was performed to increase the mRNA abundance for microarrays (NuGENTechnologies1, 2008). mRNA expression was measured (Caretti et al., 2008) using Affymetrix U133 + 2.0 microarrays (Affymetrix, 2003) with ow nloaded from https://academ ic.oup.com /hum rep/article/27/9/2747/627856 by guest on 16 June 2024 independent validation by a second Agilent 4×44 microarray platform (AgilentTechnologies, 2009) on a subset of samples. .. For data analysis of array platforms hybridized with mRNA isolated from the stromal and glandular epithelium, the Affymetrix raw data were background corrected, quantile normalized and summarized using the RMA method from the Affymetrix package from Bioconductor (Gautier et al., 2004) and Agilent data were quantile normalized.

    Biomarker Discovery:

    Article Title: Gene expression confirms a potentially receptive endometrium identified by histology in fertile women.
    Article Snippet: .. Linear polymerase chain reaction (PCR) was performed to increase the mRNA abundance for microarrays (NuGENTechnologies1, 2008). mRNA expression was measured (Caretti et al., 2008) using Affymetrix U133 + 2.0 microarrays (Affymetrix, 2003) with ow nloaded from https://academ ic.oup.com /hum rep/article/27/9/2747/627856 by guest on 16 June 2024 independent validation by a second Agilent 4×44 microarray platform (AgilentTechnologies, 2009) on a subset of samples. .. For data analysis of array platforms hybridized with mRNA isolated from the stromal and glandular epithelium, the Affymetrix raw data were background corrected, quantile normalized and summarized using the RMA method from the Affymetrix package from Bioconductor (Gautier et al., 2004) and Agilent data were quantile normalized.



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