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multiplex stained tissue microarray section  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences multiplex stained tissue microarray section
    Multiplex Stained Tissue Microarray Section, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 5463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+multiplex/PhenoImager+HT/pm41385179-63-1-13
    Average 99 stars, based on 5463 article reviews
    multiplex stained tissue microarray section - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Pdk1-dependent metabolic heterogeneity promotes aggressive colorectal cancer through cell-intrinsic interferon signaling
    Article Snippet: Opal multiplex immunofluorescent staining on the Leica Bond RX platform was performed by the IRB-Barcelona Histopathology service on formalin-fixed paraffin-embedded tissue sections (3-4 μm in thickness) using the following antibodies and Opal reagents (Akoya, NEL871001KT): anti-RFP/mCherry (Rockland, 600-401-379; ER2, 20’, 1:500) – Opal 570; anti-Ki67 (Abcam, ab15580; ER2, 20’, 1:500) – Opal 620; anti-EpCAM (E6V8Y) (Cell Signaling, 93790; ER2, 20’, 1:500) – Opal 690; and anti-CD31 (Abcam, ab28364; ER2, 20’1:200) – Opal 480. .. Immunofluorescence images were acquired with a Phenoimager HT digital scanner (Akoya Biosciences) with a 20X objective. ..

    Staining:

    Article Title: STAT3D as a new image-tailored Xenium-based spatial transcriptomics tool for accurately dissecting the tumor microenvironment.
    Article Snippet: Chromogenic IHC staining was performed manually following ImmPRESS guidelines, using antibodies against Cytokeratin (HistoLab, AE-1/AE-3) at a dilution of 1:300, LAMP3 (Abcam, ab1010E1.01) at 1:50, CD8a (Thermo Fisher Scientific, OTI3h6) at 1:1000, CCR7 (Abcam, ab253187) at 1:250, and IDO1 (Abcam, Ab211017) at 1:500. .. Staining intensity was evaluated using a PhenoImager HT (1.0.13, Akoya Bioscience, Marlborough, MA) microscope at 20 × and 40 × magnification. ..

    Article Title: Pericyte loss and endothelial activation define distinct vascular states in cerebral small vessel disease.
    Article Snippet: .. Stained tissue sections were imaged at 20 × magnification using the PhenoImager HT Automated Quantitative Pathology Imaging System (Akoya Biosciences). ..

    Microscopy:

    Article Title: STAT3D as a new image-tailored Xenium-based spatial transcriptomics tool for accurately dissecting the tumor microenvironment.
    Article Snippet: Chromogenic IHC staining was performed manually following ImmPRESS guidelines, using antibodies against Cytokeratin (HistoLab, AE-1/AE-3) at a dilution of 1:300, LAMP3 (Abcam, ab1010E1.01) at 1:50, CD8a (Thermo Fisher Scientific, OTI3h6) at 1:1000, CCR7 (Abcam, ab253187) at 1:250, and IDO1 (Abcam, Ab211017) at 1:500. .. Staining intensity was evaluated using a PhenoImager HT (1.0.13, Akoya Bioscience, Marlborough, MA) microscope at 20 × and 40 × magnification. ..

    Article Title: Multiplex hyperspectral IHC: A 12-plex assay for high-dimensional analysis of the tumor microenvironment.
    Article Snippet: .. Other hyperspectral commercial bright field microscopy systems could also be used to image the slides such as with the Mantra or PhenoImager HT produced by Akoya Biosciences. ..

    Produced:

    Article Title: Multiplex hyperspectral IHC: A 12-plex assay for high-dimensional analysis of the tumor microenvironment.
    Article Snippet: .. Other hyperspectral commercial bright field microscopy systems could also be used to image the slides such as with the Mantra or PhenoImager HT produced by Akoya Biosciences. ..

    Immunohistochemistry:

    Article Title: EGFR-targeted antibody-toxin conjugate in recurrent glioma: a phase 1 trial.
    Article Snippet: D2C7-ATC is a recombinant antibody−toxin conjugate (ATC) targeting wild-type epidermal growth factor receptor (EGFR) and mutant EGFR variant III (EGFRvIII).. We conducted a single-arm, single-site, open-label phase 1 trial evaluating a single infusion of D2C7-ATC via convection-enhanced delivery in the enhancing disease of 81 patients with biopsy-confirmed recurrent malignant glioma.. The primary objective was to determine toxicity and the recommended phase 2 dose (RP2D) of D2C7-ATC.

    Imaging:

    Article Title: Macrophage-derived MMP14 fosters immunosuppressive tumor microenvironment by regulating soluble semaphorin 4D
    Article Snippet: All the slides were counterstained with Cell Death Discovery spectral DAPI (Akoya Biosciences) and mounted with an anti-fade fluorescence mounting medium (ab104135, Abcam). .. Multichannel imaging was performed using the Vectra Polaris Imaging System (Akoya Biosciences). ..

    Article Title: Pericyte loss and endothelial activation define distinct vascular states in cerebral small vessel disease.
    Article Snippet: .. Stained tissue sections were imaged at 20 × magnification using the PhenoImager HT Automated Quantitative Pathology Imaging System (Akoya Biosciences). ..

    Tissue:

    Article Title: Pericyte loss and endothelial activation define distinct vascular states in cerebral small vessel disease.
    Article Snippet: .. Stained tissue sections were imaged at 20 × magnification using the PhenoImager HT Automated Quantitative Pathology Imaging System (Akoya Biosciences). ..

    Automated:

    Article Title: Pericyte loss and endothelial activation define distinct vascular states in cerebral small vessel disease.
    Article Snippet: .. Stained tissue sections were imaged at 20 × magnification using the PhenoImager HT Automated Quantitative Pathology Imaging System (Akoya Biosciences). ..

    Pathology:

    Article Title: Pericyte loss and endothelial activation define distinct vascular states in cerebral small vessel disease.
    Article Snippet: .. Stained tissue sections were imaged at 20 × magnification using the PhenoImager HT Automated Quantitative Pathology Imaging System (Akoya Biosciences). ..



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    Thermo Fisher isac multiplex microarray isac chip
    (A–C) The odds ratio with 95% confidence interval between (A) blood eosinophilia (%), (B) FeNO, and (C) lung function measurements and >0.3 <t>ISAC‐standardized</t> units sensitization to individual IgE components of furry animals. Blood eosinophil count ≥4% was considered eosinophilia. ≥40% increase in R5 in IOS, ≥12% decrease in FEV1 in spirometria, or both were interpreted as positive BDT. FeNO z ‐score ≥2 SD was considered positive. BDT, bronchodilation test; CI, confidence interval; FeNO, fractional concentration of exhaled nitric oxide; IOS, impulse oscillometry.
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    Image Search Results


    (A–C) The odds ratio with 95% confidence interval between (A) blood eosinophilia (%), (B) FeNO, and (C) lung function measurements and >0.3 ISAC‐standardized units sensitization to individual IgE components of furry animals. Blood eosinophil count ≥4% was considered eosinophilia. ≥40% increase in R5 in IOS, ≥12% decrease in FEV1 in spirometria, or both were interpreted as positive BDT. FeNO z ‐score ≥2 SD was considered positive. BDT, bronchodilation test; CI, confidence interval; FeNO, fractional concentration of exhaled nitric oxide; IOS, impulse oscillometry.

    Journal: Clinical and Translational Allergy

    Article Title: Pets, furry animal allergen components, and asthma in childhood

    doi: 10.1002/clt2.12337

    Figure Lengend Snippet: (A–C) The odds ratio with 95% confidence interval between (A) blood eosinophilia (%), (B) FeNO, and (C) lung function measurements and >0.3 ISAC‐standardized units sensitization to individual IgE components of furry animals. Blood eosinophil count ≥4% was considered eosinophilia. ≥40% increase in R5 in IOS, ≥12% decrease in FEV1 in spirometria, or both were interpreted as positive BDT. FeNO z ‐score ≥2 SD was considered positive. BDT, bronchodilation test; CI, confidence interval; FeNO, fractional concentration of exhaled nitric oxide; IOS, impulse oscillometry.

    Article Snippet: An ISAC multiplex microarray (ISAC Chip, Thermo Fisher Scientific) was used to analyze IgE‐sensitization to 112 different allergen components.

    Techniques: Concentration Assay

    Connection between asthma symptoms over the last year and sensitization (>0.3 ISAC standardized units) to individual IgE components of furry animals.

    Journal: Clinical and Translational Allergy

    Article Title: Pets, furry animal allergen components, and asthma in childhood

    doi: 10.1002/clt2.12337

    Figure Lengend Snippet: Connection between asthma symptoms over the last year and sensitization (>0.3 ISAC standardized units) to individual IgE components of furry animals.

    Article Snippet: An ISAC multiplex microarray (ISAC Chip, Thermo Fisher Scientific) was used to analyze IgE‐sensitization to 112 different allergen components.

    Techniques: