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surescan microarray scanner laser reader  (Agilent technologies)


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    Agilent technologies surescan microarray scanner laser reader
    Surescan Microarray Scanner Laser Reader, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+laser+scanner/pm38801463-54-16-22
    Average 90 stars, based on 1 article reviews
    surescan microarray scanner laser reader - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Epigenetic Effects of Resveratrol on Oncogenic Signaling in Breast Cancer
    Article Snippet: Alexa Flor 555 Conjugate Streptavidin (Thermo Fisher Scientific, Waltham, MA, USA) was used as a dye for detection. .. Array slides were scanned to detect florescence intensity using the Agilent G4900DA SureScan Microarray Scanner System (Agilent, Santa Clara, CA, USA). ..

    Article Title: Increased copy-number variant load of associated risk genes in sporadic cases of amyotrophic lateral sclerosis.
    Article Snippet: .. After hybridization, the array slides were washed and scanned at 3 μm resolution on a G4900DA SureScan Microarray Scanner System (Agilent Technologies, Santa Clara, CA). ..

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Potential molecular patterns for tuberculosis susceptibility in diabetic patients with poor glycaemic control: a pilot study.
    Article Snippet: Type 2 diabetes (DM2) is an increasingly prevalent disease that challenges tuberculosis (TB) control strategies worldwide.. It is significant that DM2 patients with poor glycemic control (PDM2) are prone to developing tuberculosis.. Furthermore, elucidating the molecular mechanisms that govern this susceptibility is imperative to address this problem.

    Article Title: Revealing the potential role of hsa-miR-663a in modulating the PI3K-Akt signaling pathway via miRNA microarray in spinal muscular atrophy patient fibroblast-derived iPSCs.
    Article Snippet: .. The array was analyzed using Agilent SureScan Microarray Scanner (G4900DA). .. Normalized intensities were extracted using the Agilent Feature Extraction Software and the analysis were performed using Agilent GeneSpring Analysis Software version 14.9.1.

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
    Article Snippet: After fragmentation steps following the manufacturer’s protocol, the cRNAs were immediately hybridized into the Agilent SurePrint G3 Human Gene Expression v3 8x60K for 17 h at 65 °C in a rotating microarray hybridization oven (Agilent, Santa Clara, CA, USA) prior to washing. .. Slides were immediately scanned using the Agilent SureScan Microarray Scanner (G4900DA) with 3 μM resolution at 532 nm wavelength. .. Normalized intensities were extracted using the Agilent Feature Extraction Software v9.1 and were uploaded to the Agilent GeneSpring Software v14.8 for analysis.

    Hybridization:

    Article Title: Increased copy-number variant load of associated risk genes in sporadic cases of amyotrophic lateral sclerosis.
    Article Snippet: .. After hybridization, the array slides were washed and scanned at 3 μm resolution on a G4900DA SureScan Microarray Scanner System (Agilent Technologies, Santa Clara, CA). ..

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Prognostic Value of Tumor Tissue Up-regulated microRNAs in Clear Cell Renal Cell Carcinoma (ccRCC)
    Article Snippet: .. Following the hybridization, microarrays were scanned using the Agilent G4900DA scanner. .. The probe intensities were then extracted using the Agilent Feature Extractor software, version 11.5.1.1.

    Incubation:

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Fluorescence:

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Potential molecular patterns for tuberculosis susceptibility in diabetic patients with poor glycaemic control: a pilot study.
    Article Snippet: Type 2 diabetes (DM2) is an increasingly prevalent disease that challenges tuberculosis (TB) control strategies worldwide.. It is significant that DM2 patients with poor glycemic control (PDM2) are prone to developing tuberculosis.. Furthermore, elucidating the molecular mechanisms that govern this susceptibility is imperative to address this problem.

    Glass:

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Extraction:

    Article Title: Serum miR‑29 is increased in mice with early liver fibrosis
    Article Snippet: .. The Cy3-labeled miRNA was incubated for hybridization with miRNA microarray slides (SurePrint G3 Mouse 8x60-K miRNA microarray slides (cat. no. G4872A; Agilent Technologies, Inc.) at 55 ̊C and 20 rpm for 20 h. Cy3 fluorescence signals on glass slides were detected using a SureScan microarray scanner (cat. no. G4900DA; Agilent Technologies, Inc.), and fluorescence quantification was done with Agilent Feature Extraction 12.0 (Agilent Technologies, Inc.). .. As a method of evaluating Spike-In, Agilent Feature Extraction 12.0 was used to ensure that the calculated values of LabelingSpike-InSignal and HybSpike-InSignal were both >2.5.

    Article Title: Potential molecular patterns for tuberculosis susceptibility in diabetic patients with poor glycaemic control: a pilot study.
    Article Snippet: Type 2 diabetes (DM2) is an increasingly prevalent disease that challenges tuberculosis (TB) control strategies worldwide.. It is significant that DM2 patients with poor glycemic control (PDM2) are prone to developing tuberculosis.. Furthermore, elucidating the molecular mechanisms that govern this susceptibility is imperative to address this problem.



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    Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Journal: BMC Cancer

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    doi: 10.1186/s12885-024-12794-6

    Figure Lengend Snippet: Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Article Snippet: The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 µm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions.

    Techniques: Microarray, Transfection, Plasmid Preparation, Expressing, Over Expression, Control, Binding Assay, In Silico

    The prediction information between miR-141 and targeted genes identified by microarray analysis using in-silico miRWalk tool

    Journal: BMC Cancer

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    doi: 10.1186/s12885-024-12794-6

    Figure Lengend Snippet: The prediction information between miR-141 and targeted genes identified by microarray analysis using in-silico miRWalk tool

    Article Snippet: The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 µm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions.

    Techniques: Microarray, Binding Assay