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sample preparation and microarray hybridization protocols  (Arraystar inc)

 
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    Structured Review

    Arraystar inc sample preparation and microarray hybridization protocols
    Sample Preparation And Microarray Hybridization Protocols, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+protocols/microarray+hybridization/pm39695693-64-4-13
    Average 90 stars, based on 1 article reviews
    sample preparation and microarray hybridization protocols - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sample Prep:

    Article Title: Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.
    Article Snippet: .. All sample preparation and microarray hybridization were performed according to the protocols of Arraystar (Rockville, MD, USA). .. Briefly, circRNAs were enriched by removing linear RNAs with Rnase R. The enriched circRNAs were then amplified and transcribed into fluorescent cRNA with a random priming method (Arraystar Super RNA Labeling Kit).

    Article Title: Circular RNA circTATDN3 promotes the Warburg effect and proliferation in colorectal cancer.
    Article Snippet: As one of the key metabolic enzymes in the glycolytic pathway, lactate dehydrogenase A (LDHA) might be linked to tumor proliferation by driving the Warburg effect.. Circular RNAs (circRNAs) are widely implicated in tumor progression.. Here, we report that circTATDN3, a circular RNA that interacts with LDHA, plays a critical role in proliferation and energy metabolism in CRC.

    Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
    Article Snippet: The total RNA of each sample was quantified by the NanoDrop ND-1000 spectrophotometer, and the integrity of RNA was evaluated by the Bioanalyzer 2100 or Mops electrophoresis. .. Following Arraystar’s standard protocol, sample preparation and microarray hybridization were executed. ..

    Article Title: CircXPO6 promotes breast cancer progression through competitively inhibiting the ubiquitination degradation of c-Myc.
    Article Snippet: The number of breast cancer (BC) patients is increasing year by year, which is severely endangering to human life and health. c-Myc is a transcription factor, studies have shown that it is a very significant factor in tumor progression, but how it is regulated in BC is still not well understood.. Here, we used the RIP microarray sequencing to confirm circXPO6, which had a high affinity with c-Myc and highly expressed in triple-negative breast cancer (TNBC) tissues and cells.. CircXPO6 overexpression promoted tumor growth in vivo and in vitro.

    Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
    Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

    Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
    Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

    Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
    Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

    Microarray:

    Article Title: Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.
    Article Snippet: .. All sample preparation and microarray hybridization were performed according to the protocols of Arraystar (Rockville, MD, USA). .. Briefly, circRNAs were enriched by removing linear RNAs with Rnase R. The enriched circRNAs were then amplified and transcribed into fluorescent cRNA with a random priming method (Arraystar Super RNA Labeling Kit).

    Article Title: Circular RNA circTATDN3 promotes the Warburg effect and proliferation in colorectal cancer.
    Article Snippet: As one of the key metabolic enzymes in the glycolytic pathway, lactate dehydrogenase A (LDHA) might be linked to tumor proliferation by driving the Warburg effect.. Circular RNAs (circRNAs) are widely implicated in tumor progression.. Here, we report that circTATDN3, a circular RNA that interacts with LDHA, plays a critical role in proliferation and energy metabolism in CRC.

    Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
    Article Snippet: The total RNA of each sample was quantified by the NanoDrop ND-1000 spectrophotometer, and the integrity of RNA was evaluated by the Bioanalyzer 2100 or Mops electrophoresis. .. Following Arraystar’s standard protocol, sample preparation and microarray hybridization were executed. ..

    Article Title: CircXPO6 promotes breast cancer progression through competitively inhibiting the ubiquitination degradation of c-Myc.
    Article Snippet: The number of breast cancer (BC) patients is increasing year by year, which is severely endangering to human life and health. c-Myc is a transcription factor, studies have shown that it is a very significant factor in tumor progression, but how it is regulated in BC is still not well understood.. Here, we used the RIP microarray sequencing to confirm circXPO6, which had a high affinity with c-Myc and highly expressed in triple-negative breast cancer (TNBC) tissues and cells.. CircXPO6 overexpression promoted tumor growth in vivo and in vitro.

    Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
    Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

    Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
    Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

    Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
    Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

    Hybridization:

    Article Title: Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.
    Article Snippet: .. All sample preparation and microarray hybridization were performed according to the protocols of Arraystar (Rockville, MD, USA). .. Briefly, circRNAs were enriched by removing linear RNAs with Rnase R. The enriched circRNAs were then amplified and transcribed into fluorescent cRNA with a random priming method (Arraystar Super RNA Labeling Kit).

    Article Title: Circular RNA circTATDN3 promotes the Warburg effect and proliferation in colorectal cancer.
    Article Snippet: As one of the key metabolic enzymes in the glycolytic pathway, lactate dehydrogenase A (LDHA) might be linked to tumor proliferation by driving the Warburg effect.. Circular RNAs (circRNAs) are widely implicated in tumor progression.. Here, we report that circTATDN3, a circular RNA that interacts with LDHA, plays a critical role in proliferation and energy metabolism in CRC.

    Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
    Article Snippet: The total RNA of each sample was quantified by the NanoDrop ND-1000 spectrophotometer, and the integrity of RNA was evaluated by the Bioanalyzer 2100 or Mops electrophoresis. .. Following Arraystar’s standard protocol, sample preparation and microarray hybridization were executed. ..

    Article Title: CircXPO6 promotes breast cancer progression through competitively inhibiting the ubiquitination degradation of c-Myc.
    Article Snippet: The number of breast cancer (BC) patients is increasing year by year, which is severely endangering to human life and health. c-Myc is a transcription factor, studies have shown that it is a very significant factor in tumor progression, but how it is regulated in BC is still not well understood.. Here, we used the RIP microarray sequencing to confirm circXPO6, which had a high affinity with c-Myc and highly expressed in triple-negative breast cancer (TNBC) tissues and cells.. CircXPO6 overexpression promoted tumor growth in vivo and in vitro.

    Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
    Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

    Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
    Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

    Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
    Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.



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    Image Search Results


    (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Microarray, Expressing, Transfection, shRNA, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Recombinant, Microarray, shRNA, Software