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data analysis microarray slides  (Danaher Inc)


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    Danaher Inc data analysis microarray slides
    Data Analysis Microarray Slides, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+data+analysis+slides/GenePix+Microarray+Systems/pm20193762-131-2-13
    Average 94 stars, based on 460 article reviews
    data analysis microarray slides - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Microarray:

    Article Title: Unraveling the role of HDAC3 as an immunotherapy prognostic biomarker and therapeutic target in advanced non-small cell lung cancer
    Article Snippet: The microarray was retrieved from − 80 °C storage and blocked with bovine serum albumin (BSA, 9048-46-8, Sigma) for 1.5 h. Following the blocking step, the microarray was incubated with plasma samples at a 1:1000 dilution for 1 h. After washing with 0.1% phosphate-buffered saline-Tween 20, the plates were incubated with Alexa Fluor 647 goat anti-human IgG (109-605-003, Jackson) diluted in 5% BSA. .. The microarray was then scanned using a GenePix 4300 A microarray scanner (141095, Molecular Devices) with a 635 nm excitation laser. .. Signal intensities of IgG for each protein were quantified using GenePix Pro v.6.0 software (Molecular Devices).

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Article Title: Supporting Information _ Lee and Fan _ Yale
    Article Snippet: .. An Axon Genepix microarray scanner 4000B (Molecular Devices, USA) was used to image DRAQ5-stained cells (emitting 635 nm) on the STRQNW arrays. ..

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    other:

    Article Title: Development of Lamprey-derived Antibodies Against Human Blood Group Antigens.
    Article Snippet: A major challenge in the glycosciences is the scarcity of sensitive and specific glycanbinding reagents, such as monoclonal antibodies, for detecting and isolating glycans.. Here we report the development and characterization of new monoclonal antibodies (mAbs) that bind carbohydrate-based red blood cell (RBC) antigens including the ABO(H) antigens.. This approach exploits the immune system of the sea lamprey (Petromyzon marinus), which strongly responds to human glycans to enable the generation of high affinity antibodies.

    Article Title: Development of lamprey-derived antibodies against human blood group antigens
    Article Snippet: The scans were analyzed using GenePix (Molecular Devices) software and GLAD (GLycan Array Dashboard) software ( ).

    Binding Assay:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Incubation:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Software:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Fluorescence:

    Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development
    Article Snippet: After being washed three times and air-dried through brief centrifugation, the slides were scanned using a Genepix 4200A scanner (Molecular Devices, CA, USA). .. The median foreground and background fluorescence intensities were measured for each spot on the protein microarrays using GenePix Pro 6.0 (Molecular Devices, CA, USA). ..



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    Danaher Inc microarray data analysis
    FIG. 1. MKP-1 mRNA levels are up- regulated by hypoxia in <t>microarray</t> analysis. PC12 cells were exposed to nor- moxia or hypoxia (1% O2) for 6 h, and total RNA was isolated as described under “Ex- perimental Procedures.” A, comparison of control versus control gene expression pattern. Cy3- and Cy5-labeled probes were each generated from RNA derived from cells maintained in normoxia. Probes were hybridized to arrays as de- scribed under “Experimental Proce- dures.” Background-subtracted median pixel intensities are plotted from a repre- sentative experiment. Each symbol repre- sents one spot on the array. B, compari- son of control versus hypoxia gene expression pattern. The Cy5-labeled probe was generated from RNA derived from cells maintained in normoxia, whereas the Cy3-labeled probe was gen- erated from RNA derived from cells ex- posed to hypoxia. The data shown are rep- resentative of results obtained in three separate hybridization experiments.
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    Image Search Results


    FIG. 1. MKP-1 mRNA levels are up- regulated by hypoxia in microarray analysis. PC12 cells were exposed to nor- moxia or hypoxia (1% O2) for 6 h, and total RNA was isolated as described under “Ex- perimental Procedures.” A, comparison of control versus control gene expression pattern. Cy3- and Cy5-labeled probes were each generated from RNA derived from cells maintained in normoxia. Probes were hybridized to arrays as de- scribed under “Experimental Proce- dures.” Background-subtracted median pixel intensities are plotted from a repre- sentative experiment. Each symbol repre- sents one spot on the array. B, compari- son of control versus hypoxia gene expression pattern. The Cy5-labeled probe was generated from RNA derived from cells maintained in normoxia, whereas the Cy3-labeled probe was gen- erated from RNA derived from cells ex- posed to hypoxia. The data shown are rep- resentative of results obtained in three separate hybridization experiments.

    Journal: Journal of Biological Chemistry

    Article Title: Hypoxia-induced Regulation of MAPK Phosphatase-1 as Identified by Subtractive Suppression Hybridization and cDNA Microarray Analysis

    doi: 10.1074/jbc.m103346200

    Figure Lengend Snippet: FIG. 1. MKP-1 mRNA levels are up- regulated by hypoxia in microarray analysis. PC12 cells were exposed to nor- moxia or hypoxia (1% O2) for 6 h, and total RNA was isolated as described under “Ex- perimental Procedures.” A, comparison of control versus control gene expression pattern. Cy3- and Cy5-labeled probes were each generated from RNA derived from cells maintained in normoxia. Probes were hybridized to arrays as de- scribed under “Experimental Proce- dures.” Background-subtracted median pixel intensities are plotted from a repre- sentative experiment. Each symbol repre- sents one spot on the array. B, compari- son of control versus hypoxia gene expression pattern. The Cy5-labeled probe was generated from RNA derived from cells maintained in normoxia, whereas the Cy3-labeled probe was gen- erated from RNA derived from cells ex- posed to hypoxia. The data shown are rep- resentative of results obtained in three separate hybridization experiments.

    Article Snippet: Microarray Data Analysis—Slides were scanned with a GenePix 4000A scanner (Axon Instruments, Inc., Foster City, CA) at 532 nm (Cy3) and 635 nm (Cy5) simultaneously.

    Techniques: Microarray, Isolation, Comparison, Control, Gene Expression, Labeling, Generated, Derivative Assay, Hybridization