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microarray chambers  (Agilent technologies)


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    Structured Review

    Agilent technologies microarray chambers
    Microarray Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+chambers/pmc10222845-207-19-18
    Average 90 stars, based on 1 article reviews
    microarray chambers - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:


    Article Title: Profiling transcriptional heterogeneity with Seq-Well S 3 : A low-cost, portable, high-fidelity platform for massively-parallel single-cell RNA-seq
    Article Snippet: 28 28 Platform for X-Y Rotator (Thermo-Fisher Cat. No. 88880106).

    Article Title: Promoter variants for protein production
    Article Snippet: The Gene Expression Hybridisation Kit (Agilent, Cat. No. 5188-5242) was used for hybridisation of the labelled sample cRNAs.

    Article Title: Systematic detection of m 6 A-modified transcripts at single-molecule and single-cell resolution.
    Article Snippet: For the detection and quantification ofm6A and cap-adjacentm6Am in 2x polyA+RNA, 400 ng of 2x polyA+RNAwas decapped using 25 Units of RppH (NEB, Cat. #M0356S) in 1X ThermoPol buffer (NEB, Cat. #B9004S) for 3 hours at 37 C, followed by clean up with Zymo RNAClean and Concentrator-5 Kit (Cat. #R1013).

    Article Title: miR-337-3p and Its Targets STAT3 and RAP1A Modulate Taxane Sensitivity in Non-Small Cell Lung Cancers
    Article Snippet: Protein expression levels were quantified using MicroVigene (Vigene Tech, Carlisle, MA) software and normalized for differences in protein loading using Sypro Ruby (Life Technologies) protein stain signals obtained on a separate slide printed in the same batch.

    Hybridization:

    Article Title: Whole Blood Transcriptomics in Cardiac Surgery Identifies a Gene Regulatory Network Connecting Ischemia Reperfusion with Systemic Inflammation
    Article Snippet: Fragmentation was stopped by adding 55 μl 2x GEx hybridisation buffer HI-RPM leading to a final volume of 110 μl (Gene expression hybridization kit, Agilent #5188–5242). .. Chip hybridization was performed using the gasket/slide-sandwich system and the hybridisation chamber (Agilent, #G2534A) according to manufacturer instructions. .. During hybridisation slides were rotated at 10 rpm and 65°C for 16 h. Microarray slides were washed 1 min in GE Wash Buffer 1 (Agilent, #5188–5326) at room temperature, 1 min in GE Wash Buffer 2 (Agilent, #5188–5326) at room temperature and 30s in Acetonitril at room temperature on a magnetic stirrer.

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

    Microarray:

    Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
    Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

    Control:

    Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
    Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

    Software:

    Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
    Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

    Labeling:

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

    Gene Expression:

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.



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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing