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Ebiogen Inc microarray data analysis
Microarray Data Analysis, supplied by Ebiogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+and+data+analysis/cdna+microarray/pm39567419-46-0-6
Average 90 stars, based on 1 article reviews
microarray data analysis - by Bioz Stars, 2026-10
90/100 stars

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Microarray:

Article Title: Circular RNAs trigger nonsense-mediated mRNA decay.
Article Snippet: Primary antibodies against the following proteins were purchased and used for IP, RNA-IP, or western blotting [listed in the format ‘‘protein name (catalog number, supplier)’’]: HA (11867431001, Roche), b-actin (A5441, Sigma-Aldrich), eIF4A3 (sc-365549, Santa Cruz Biotechnology), Y14 (MAB2484, Abnova), MAGOH (ab38768, Abcam), MLN51 (ab90651, Abcam), GAPDH (LF-PA0212, AbFrontier), SMG5 (ab33033, Abcam), STAU1 (A303-956A, Bethyl Laboratories), ATG5 (12994, Cell Signaling Technology), LC3B (3868, Cell Signaling Technology), cleaved-PARP (9541, Cell Signaling Technology), BCL2L11 (2933, Cell Signaling Technology), eIF4E (610269, BD Biosciences; for western blotting), HRSP12 (PA5-31352, Thermo Fisher Scientific), POP1 (12029-1-AP, Proteintech), ADAR1 (ab168809, Abcam), Drosha (ab245398, Abcam), Dicer (PA5-17021, Invitrogen), AGO1 (5053, Cell Signaling Technology), AGO2 (07-590, Merck Millipore), AGO3 (SAB4200112, Sigma-Aldrich), PACT (sc-377103, Santa Cruz Biotechnology), and TRBP (15753-1-AP, Proteintech). .. Microarray analysis was performed by Ebiogen, Inc. (South Korea). .. Briefly, poly(A)+ RNA microarray and circRNA microarray analyses were conducted using the Affymetrix gene chip (ebiogen Inc.) and ArrayStar gene chip (ArrayStar Inc., MD, USA), respectively.

Article Title: Adipose-derived stem cell-based anti-inflammatory paracrine factor regulation for the treatment of inflammatory bowel disease.
Article Snippet: Stem cell-based therapies offer promising avenues for treating inflammatory diseases owing to their immunomodulatory properties.. However, challenges persist regarding their survival and efficacy in inflamed tissues.. Our study introduces a novel approach by engineering adipose-derived stem cells (ADSCs) to enhance their viability in inflammatory environments and boost the secretion of paracrine factors for treating inflammatory bowel disease (IBD).

Article Title: Oncogenic fusion of CD63-BCAR4 contributes cancer stem cell-like properties via ALDH1 activity.
Article Snippet: Labeled cells were analyzed by using flow cytometry (BD FACSLyric, BD Biosciences) and analyzed using the FlowJo software (BD). .. The total RNA from each cell line was analyzed using an RNA Microarray provided by Ebiogen Inc. .. Briefly, TRIzol (Invitrogen)‐based extracted RNA was used to synthesize cDNA, and the amplified cRNA was hybridized with the Agilent Human Gene Expression 4× 44K Microarray (Agilent Technologies) according to the manufacturer's instructions.

Article Title: Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.
Article Snippet: Multikinase inhibitors, such as sorafenib, are used for the treatment of advanced carcinomas but the response shows limited efficacy or varies a lot with patients.. Here we adopted the systems approach combined with high-throughput data analysis to discover key mechanism embedded in the drug response.. When analyzing the transcriptomic data from the Cancer Cell Line Encyclopedia (CCLE) database, endothelin 1 (EDN1) was enriched in cancer cells with low responsiveness to sorafenib.

Article Title: AURKB, in concert with REST, acts as an oxygen-sensitive epigenetic regulator of the hypoxic induction of MDM2
Article Snippet: ChIP assay (Merck, Darmstadt, Germany) and RT-qPCR (Enzynomics, Daejeon, South Korea) kits were used as per the manufacturer’s instructions. contains the primer sequences for PCR. .. DNA microarray was performed commercially by Ebiogen (Seoul, South Korea). ..

Article Title: The facilitated osteogenic differentiation by extracellular proline treatment in in vitro cell cultivation using MC3T3E1 and hPDLF.
Article Snippet: Collagen proteins are the most dominant component of the extracellular matrix.. These collagen proteins are expected to function as ground substances to maintain the integrity of connective tissues such as bones, cartilage, muscles, and skin (Selvaraj et al. 2024).. The detailed gene expressions and physiological regulations of collagen biosynthesis have been well studied alongside related gene expression patterns and physiological productions with secretion, assembling, and turnover to understand the development of physiology and tissue regeneration.

Article Title: Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells
Article Snippet: For the microarray experiment, total RNAs were isolated from MG-63 cultured on a parylene N-coated or control plate using a RNeasy Mini Kit (Qiagen, Hilden, Germany). .. The commercial microarray service was performed in eBiogen (Seoul, Republic of Korea). .. High-throughput sequencing was performed as single-end 75 sequencing using NextSeq 500 (Illumina).

Article Title: Simplified Brain Organoids for Rapid and Robust Modeling of Brain Disease
Article Snippet: .. For comprehensive gene expression analysis, 12 organoids were used for each sample and cDNA microarray was performed at e-biogen (ebiogen, Seoul, South Korea) using an Agilent Human GE 4 × 44K v2 Microarray Kit (Agilent, Santa Clara, CA, United States), as described previously ( ). .. Data normalization was performed with GeneSpring software (Agilent) and differentially expressed genes were sorted by fold-change of gene expression and heat map was presented using the Multi Experiment Viewer software (MeV version 4.9.0 ).

Isolation:

Article Title: Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.
Article Snippet: Multikinase inhibitors, such as sorafenib, are used for the treatment of advanced carcinomas but the response shows limited efficacy or varies a lot with patients.. Here we adopted the systems approach combined with high-throughput data analysis to discover key mechanism embedded in the drug response.. When analyzing the transcriptomic data from the Cancer Cell Line Encyclopedia (CCLE) database, endothelin 1 (EDN1) was enriched in cancer cells with low responsiveness to sorafenib.

Amplification:

Article Title: Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.
Article Snippet: Multikinase inhibitors, such as sorafenib, are used for the treatment of advanced carcinomas but the response shows limited efficacy or varies a lot with patients.. Here we adopted the systems approach combined with high-throughput data analysis to discover key mechanism embedded in the drug response.. When analyzing the transcriptomic data from the Cancer Cell Line Encyclopedia (CCLE) database, endothelin 1 (EDN1) was enriched in cancer cells with low responsiveness to sorafenib.

Gene Expression:

Article Title: Simplified Brain Organoids for Rapid and Robust Modeling of Brain Disease
Article Snippet: .. For comprehensive gene expression analysis, 12 organoids were used for each sample and cDNA microarray was performed at e-biogen (ebiogen, Seoul, South Korea) using an Agilent Human GE 4 × 44K v2 Microarray Kit (Agilent, Santa Clara, CA, United States), as described previously ( ). .. Data normalization was performed with GeneSpring software (Agilent) and differentially expressed genes were sorted by fold-change of gene expression and heat map was presented using the Multi Experiment Viewer software (MeV version 4.9.0 ).



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