Amplification:Article Title: Channel synapse mediates neurotransmission of airway protective chemoreflexes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Pkd2l1-Cre Huang et al.71 N/A Mouse: Pou2f3 KO Matsumoto et al.32 N/A Mouse: Pou2f3mCherry-P2A-CreERT2 This Paper N/A Mouse: TIGRE2.0-ASAP4e This Paper N/A Mouse: TIGRE2.0-GRABatp1.0 This Paper N/A Mouse: Trpm5 KO This Paper N/A Hartley guinea pig Shimizu Laboratory Supplies N/A Oligonucleotides TSO for Smart-seq3: 50-biotin-AGAGACA GATTGCGCAATGNNNNNNNNrGrGrG-30 M Hagemann-Jensen et al.72 N/A OligodT30VN for Smart-seq3: 50-biotinACGAGCATCAGCAGCATACGA T30VN-30 M Hagemann-Jensen et al.72 N/A Primers for linear amplification and PCR-based target enrichment, see the Method section This paper N/A Software and algorithms zUMIs Parekh et al.73 https://github.com/sdparekh/zUMIs STAR Dobin et al.74 https://github.com/alexdobin/STAR Cell Ranger 10x Genomics https://www.10xgenomics.com/jp/ support/software/cell-ranger/latest Seurat Hao et al.75 https://satijalab.org/seurat/ Fiji - ImageJ 2.0.0- NIH RRID: SCR_002285; http://fiji.sc ZEISS ZEN 3.2 (blue edition) Carl Zeiss RRID: SCR_013672 VS200 ASW Evident https://www.olympus-lifescience.com/ BZ-X Viewer Keyence https://www.keyence.co.jp/ Lab Chart AD Instruments RRID: SCR_017551; https://www.adinstruments.com/ products/labchart MATLAB MathWorks RRID: SCR_001622; http://www.mathworks.com/ products/matlab/ Python Python Software Foundation RRID: SCR_008394; http://www.python.org/ Spike2 software Cambridge Electronic Design RRID: SCR_000903 IGOR Pro waveMetrics RRID: SCR_000325 G*Power Faul et al.76 RRID: SCR_013726; http://www.gpower.hhu.de/ FV31S-SW Evident https://evidentscientific.com/en/downloads CNMFe Zhou et al.77 https://github.com/zhoupc/CNMF_E RStudio version 2023.06.0 Posit RRID: SCR_000432 R R Core Team https://www.r-project.org/ ll OPEN ACCESS Please cite this article in press as: Soma et al., Channel synapse mediates neurotransmission of airway protective chemoreflexes, Cell (2025), https://doi.org/10.1016/j.cell.2025.03.007 ..
Polymerase Chain Reaction:Article Title: Channel synapse mediates neurotransmission of airway protective chemoreflexes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Pkd2l1-Cre Huang et al.71 N/A Mouse: Pou2f3 KO Matsumoto et al.32 N/A Mouse: Pou2f3mCherry-P2A-CreERT2 This Paper N/A Mouse: TIGRE2.0-ASAP4e This Paper N/A Mouse: TIGRE2.0-GRABatp1.0 This Paper N/A Mouse: Trpm5 KO This Paper N/A Hartley guinea pig Shimizu Laboratory Supplies N/A Oligonucleotides TSO for Smart-seq3: 50-biotin-AGAGACA GATTGCGCAATGNNNNNNNNrGrGrG-30 M Hagemann-Jensen et al.72 N/A OligodT30VN for Smart-seq3: 50-biotinACGAGCATCAGCAGCATACGA T30VN-30 M Hagemann-Jensen et al.72 N/A Primers for linear amplification and PCR-based target enrichment, see the Method section This paper N/A Software and algorithms zUMIs Parekh et al.73 https://github.com/sdparekh/zUMIs STAR Dobin et al.74 https://github.com/alexdobin/STAR Cell Ranger 10x Genomics https://www.10xgenomics.com/jp/ support/software/cell-ranger/latest Seurat Hao et al.75 https://satijalab.org/seurat/ Fiji - ImageJ 2.0.0- NIH RRID: SCR_002285; http://fiji.sc ZEISS ZEN 3.2 (blue edition) Carl Zeiss RRID: SCR_013672 VS200 ASW Evident https://www.olympus-lifescience.com/ BZ-X Viewer Keyence https://www.keyence.co.jp/ Lab Chart AD Instruments RRID: SCR_017551; https://www.adinstruments.com/ products/labchart MATLAB MathWorks RRID: SCR_001622; http://www.mathworks.com/ products/matlab/ Python Python Software Foundation RRID: SCR_008394; http://www.python.org/ Spike2 software Cambridge Electronic Design RRID: SCR_000903 IGOR Pro waveMetrics RRID: SCR_000325 G*Power Faul et al.76 RRID: SCR_013726; http://www.gpower.hhu.de/ FV31S-SW Evident https://evidentscientific.com/en/downloads CNMFe Zhou et al.77 https://github.com/zhoupc/CNMF_E RStudio version 2023.06.0 Posit RRID: SCR_000432 R R Core Team https://www.r-project.org/ ll OPEN ACCESS Please cite this article in press as: Soma et al., Channel synapse mediates neurotransmission of airway protective chemoreflexes, Cell (2025), https://doi.org/10.1016/j.cell.2025.03.007 ..
Software:Article Title: Channel synapse mediates neurotransmission of airway protective chemoreflexes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Pkd2l1-Cre Huang et al.71 N/A Mouse: Pou2f3 KO Matsumoto et al.32 N/A Mouse: Pou2f3mCherry-P2A-CreERT2 This Paper N/A Mouse: TIGRE2.0-ASAP4e This Paper N/A Mouse: TIGRE2.0-GRABatp1.0 This Paper N/A Mouse: Trpm5 KO This Paper N/A Hartley guinea pig Shimizu Laboratory Supplies N/A Oligonucleotides TSO for Smart-seq3: 50-biotin-AGAGACA GATTGCGCAATGNNNNNNNNrGrGrG-30 M Hagemann-Jensen et al.72 N/A OligodT30VN for Smart-seq3: 50-biotinACGAGCATCAGCAGCATACGA T30VN-30 M Hagemann-Jensen et al.72 N/A Primers for linear amplification and PCR-based target enrichment, see the Method section This paper N/A Software and algorithms zUMIs Parekh et al.73 https://github.com/sdparekh/zUMIs STAR Dobin et al.74 https://github.com/alexdobin/STAR Cell Ranger 10x Genomics https://www.10xgenomics.com/jp/ support/software/cell-ranger/latest Seurat Hao et al.75 https://satijalab.org/seurat/ Fiji - ImageJ 2.0.0- NIH RRID: SCR_002285; http://fiji.sc ZEISS ZEN 3.2 (blue edition) Carl Zeiss RRID: SCR_013672 VS200 ASW Evident https://www.olympus-lifescience.com/ BZ-X Viewer Keyence https://www.keyence.co.jp/ Lab Chart AD Instruments RRID: SCR_017551; https://www.adinstruments.com/ products/labchart MATLAB MathWorks RRID: SCR_001622; http://www.mathworks.com/ products/matlab/ Python Python Software Foundation RRID: SCR_008394; http://www.python.org/ Spike2 software Cambridge Electronic Design RRID: SCR_000903 IGOR Pro waveMetrics RRID: SCR_000325 G*Power Faul et al.76 RRID: SCR_013726; http://www.gpower.hhu.de/ FV31S-SW Evident https://evidentscientific.com/en/downloads CNMFe Zhou et al.77 https://github.com/zhoupc/CNMF_E RStudio version 2023.06.0 Posit RRID: SCR_000432 R R Core Team https://www.r-project.org/ ll OPEN ACCESS Please cite this article in press as: Soma et al., Channel synapse mediates neurotransmission of airway protective chemoreflexes, Cell (2025), https://doi.org/10.1016/j.cell.2025.03.007 ..
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