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massarray® data  (agena bioscience)


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    Structured Review

    agena bioscience massarray® data
    Massarray® Data, supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray%C2%AE+data/massarray+system/pm38270218-78-0-9
    Average 90 stars, based on 1 article reviews
    massarray® data - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: LncRNA C5orf64 polymorphisms (rs12518552 and rs2950218) decreases pulmonary tuberculosis susceptibility
    Article Snippet: The polymerase chain reaction (PCR) and single base extension (SBE) primers for these SNPs were designed via Agena’s tool and synthesized by Sangon Biotech (Shanghai), with sequences in Supplement Table 1 . .. The PCR thermal conditions were as follows: initial denaturation at 94 °C for 15 min, followed by 45 cycles of (denaturation at 94 °C for 20 s, annealing at 56 °C for 30 s, and extension at 72 °C for 1 min), with a final extension at 72 °C for 3 min. SNP genotyping was carried out on the Agena MassARRAY system per the manufacturer’s instructions, with results visualized via Agena Bioscience TYPER software (v4.0). ..

    Article Title: Impact of body weight on mycophenolic acid population pharmacokinetics in paediatric lupus nephritis: a pharmacogenomic integration study.
    Article Snippet: Whole blood samples were used for genomic DNA isolation, with DNA extracted using the TIANGEN Blood Genome Extraction Kit (TIANGEN, Beijing, China). .. All 54 SNPs were analysed employing the previously published Agena MassARRAY System technique (Agena, California, USA) according to the manufacturer’s instructions.17 The PCR primers were designed using Agena Bioscience Assay Design software and are listed in online supplemental table 2. ..

    Software:

    Article Title: LncRNA C5orf64 polymorphisms (rs12518552 and rs2950218) decreases pulmonary tuberculosis susceptibility
    Article Snippet: The polymerase chain reaction (PCR) and single base extension (SBE) primers for these SNPs were designed via Agena’s tool and synthesized by Sangon Biotech (Shanghai), with sequences in Supplement Table 1 . .. The PCR thermal conditions were as follows: initial denaturation at 94 °C for 15 min, followed by 45 cycles of (denaturation at 94 °C for 20 s, annealing at 56 °C for 30 s, and extension at 72 °C for 1 min), with a final extension at 72 °C for 3 min. SNP genotyping was carried out on the Agena MassARRAY system per the manufacturer’s instructions, with results visualized via Agena Bioscience TYPER software (v4.0). ..

    Article Title: Impact of body weight on mycophenolic acid population pharmacokinetics in paediatric lupus nephritis: a pharmacogenomic integration study.
    Article Snippet: Whole blood samples were used for genomic DNA isolation, with DNA extracted using the TIANGEN Blood Genome Extraction Kit (TIANGEN, Beijing, China). .. All 54 SNPs were analysed employing the previously published Agena MassARRAY System technique (Agena, California, USA) according to the manufacturer’s instructions.17 The PCR primers were designed using Agena Bioscience Assay Design software and are listed in online supplemental table 2. ..

    Article Title: LncRNA C5orf64 polymorphisms (rs12518552 and rs2950218) decreases pulmonary tuberculosis susceptibility.
    Article Snippet: .. 94 °C for 15 min, followed by 45 cycles of (denaturation at 94 °C for 20 s, annealing at 56 °C for 30 s, and extension at 72 °C for 1 min), with a final extension at 72 °C for 3 min. SNP genotyping was carried out on the Agena MassARRAY system per the manufacturer’s instructions, with results visualized via Agena Bioscience TYPER software (v4.0). ..

    other:

    Article Title: Association of homozygous fat mass and obesity-associated (FTO rs9939609) gene with body mass, body mass index (BMI), and the binge eating scale in women: A cross-sectional study.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Real-time Polymerase Chain Reaction:




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    Image Search Results


    This figure shows the 4 steps of the SNPflow data analysis wizard. In brief, the first step allows the uploading of either SDS files (ABI 7900HT) or TyperAnalyzer 4.0 (MassARRAY) files. For TyperAnalyzer files, a second input field asks for the name of the result group under which results shall be grouped. The second step checks the validity of the IDs found in the uploaded and reports IDs, which are not found in the study definition and shall thus be deleted. Step 3 converts the found genotype designations to standard genotypes and allows entering the name of the result, the operator name, an optional comment and the rs-number of the SNP in HapMap and the 1000 Genomes Project. This is useful in case that a SNP ID changed between the current dbSNP release and the dbSNP release used in HapMap, as happened for some SNPs. For convenience, the result name is automatically pre-set as HapMap lookup. In multiplex assays, pagination allows navigating through the single SNPs. For quality reasons a manual confirmation of each conversion is required (Checkbox “All settings are correct”). Finally, step 4 confirms the successful analysis and brings the user to the report. Exemplary abbreviation used in this figure: NTC, Non-template control; ER, annulled samples (“error”), i.e. DNA samples present on the plates, but known to be flawed. The IDs of these samples were thus replaced with “ER” in order to avoid data collection for these samples.

    Journal: PLoS ONE

    Article Title: SNPflow: A Lightweight Application for the Processing, Storing and Automatic Quality Checking of Genotyping Assays

    doi: 10.1371/journal.pone.0059508

    Figure Lengend Snippet: This figure shows the 4 steps of the SNPflow data analysis wizard. In brief, the first step allows the uploading of either SDS files (ABI 7900HT) or TyperAnalyzer 4.0 (MassARRAY) files. For TyperAnalyzer files, a second input field asks for the name of the result group under which results shall be grouped. The second step checks the validity of the IDs found in the uploaded and reports IDs, which are not found in the study definition and shall thus be deleted. Step 3 converts the found genotype designations to standard genotypes and allows entering the name of the result, the operator name, an optional comment and the rs-number of the SNP in HapMap and the 1000 Genomes Project. This is useful in case that a SNP ID changed between the current dbSNP release and the dbSNP release used in HapMap, as happened for some SNPs. For convenience, the result name is automatically pre-set as HapMap lookup. In multiplex assays, pagination allows navigating through the single SNPs. For quality reasons a manual confirmation of each conversion is required (Checkbox “All settings are correct”). Finally, step 4 confirms the successful analysis and brings the user to the report. Exemplary abbreviation used in this figure: NTC, Non-template control; ER, annulled samples (“error”), i.e. DNA samples present on the plates, but known to be flawed. The IDs of these samples were thus replaced with “ER” in order to avoid data collection for these samples.

    Article Snippet: SNPflow is a lightweight, intuitive and easily deployable application, which processes genotype data from Sequenom MassARRAY (iPLEX) and ABI 7900HT (TaqMan, KASPar) systems and is extendible to other genotyping methods as well.

    Techniques: Multiplex Assay

    The basic information of the selected  SNPs  in this study

    Journal: BMC Oral Health

    Article Title: Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population

    doi: 10.1186/s12903-022-02051-2

    Figure Lengend Snippet: The basic information of the selected SNPs in this study

    Article Snippet: The haplotype program revealed that one SNP (rs6446693) out of the six SNPs from Sequenom Massarray data was not associated with peg-shaped teeth.

    Techniques: Variant Assay

    The MSX1  SNPs  with their minor allele frequencies and HWE P -values for cases and controls at each locus based on the (36) families

    Journal: BMC Oral Health

    Article Title: Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population

    doi: 10.1186/s12903-022-02051-2

    Figure Lengend Snippet: The MSX1 SNPs with their minor allele frequencies and HWE P -values for cases and controls at each locus based on the (36) families

    Article Snippet: The haplotype program revealed that one SNP (rs6446693) out of the six SNPs from Sequenom Massarray data was not associated with peg-shaped teeth.

    Techniques:

    Haploview plot of linkage disequilibrium (r 2 ) between (rs8670, rs12532, and rs4464513) within MSX1 located on chromosome 4p16.2 represent SNP-SNP relationship. A black diamond without a number represents complete linkage disequilibrium between SNPs (r 2 = 1)

    Journal: BMC Oral Health

    Article Title: Family and case–control genetic study of MSX1 polymorphisms in peg-shaped teeth Jordanian population

    doi: 10.1186/s12903-022-02051-2

    Figure Lengend Snippet: Haploview plot of linkage disequilibrium (r 2 ) between (rs8670, rs12532, and rs4464513) within MSX1 located on chromosome 4p16.2 represent SNP-SNP relationship. A black diamond without a number represents complete linkage disequilibrium between SNPs (r 2 = 1)

    Article Snippet: The haplotype program revealed that one SNP (rs6446693) out of the six SNPs from Sequenom Massarray data was not associated with peg-shaped teeth.

    Techniques:

    基于65样本集的MassARRAY和焦磷酸测序两种方法的相关性分析 Correlation analysis of  MassARRAY  and pyrosequencing based on 65 samples

    Journal: Journal of Southern Medical University

    Article Title: 焦磷酸测序和MassARRAY定量检测DNA甲基化在年龄推断中的差异

    doi: 10.12122/j.issn.1673-4254.2020.12.21

    Figure Lengend Snippet: 基于65样本集的MassARRAY和焦磷酸测序两种方法的相关性分析 Correlation analysis of MassARRAY and pyrosequencing based on 65 samples

    Article Snippet: A , B : Plots based on data without Z-score transformation typed from MassARRAY and pyrosequencing., : Plots based on data after Z-score transformation typed from MassARRAY and pyrosequencing.

    Techniques:

    基于62样本集的MassARRAY和焦磷酸测序两种方法的相关性分析 Correlation analysis of  MassARRAY  and pyrosequencing based on 62 samples

    Journal: Journal of Southern Medical University

    Article Title: 焦磷酸测序和MassARRAY定量检测DNA甲基化在年龄推断中的差异

    doi: 10.12122/j.issn.1673-4254.2020.12.21

    Figure Lengend Snippet: 基于62样本集的MassARRAY和焦磷酸测序两种方法的相关性分析 Correlation analysis of MassARRAY and pyrosequencing based on 62 samples

    Article Snippet: A , B : Plots based on data without Z-score transformation typed from MassARRAY and pyrosequencing., : Plots based on data after Z-score transformation typed from MassARRAY and pyrosequencing.

    Techniques:

    The IFNG single nucleotide polymorphisms (SNP) are designated according to the nucleotide position relative to the transcriptional starting site of IFNG .

    Journal: Wellcome Open Research

    Article Title: Interferon-gamma polymorphisms and risk of iron deficiency and anaemia in Gambian children

    doi: 10.12688/wellcomeopenres.15750.2

    Figure Lengend Snippet: The IFNG single nucleotide polymorphisms (SNP) are designated according to the nucleotide position relative to the transcriptional starting site of IFNG .

    Article Snippet: Agena Biosciences (formerly SEQUENOM) MassARRAY ® primer-extension definitions data for the five IFNG polymorphisms Extended datafile 3.

    Techniques:

    Genotype frequencies for  BST1 rs4698412  in PD and HC groups

    Journal: CNS Neuroscience & Therapeutics

    Article Title: BST1 rs4698412 allelic variant increases the risk of gait or balance deficits in patients with Parkinson’s disease

    doi: 10.1111/cns.13099

    Figure Lengend Snippet: Genotype frequencies for BST1 rs4698412 in PD and HC groups

    Article Snippet: The BST1 rs4698412 data were processed and analyzed using MassARRAY TYPER 4.0 software (Agena Bioscience, San Diego, CA, USA) via the Beijing Genomics Institute (BGI).

    Techniques:

    Groups × genotypes ANCOVA of ALFF. (A) Main effect of diagnostic groups on ALFF in PD and HC was shown in the left cerebellum_8 and cerebellum_9. (B) Interaction between BST1 rs4698412 alleles and diagnostic groups was found in the right lingual gyrus. These findings were obtained via two‐way factorial analysis of covariance (ANCOVA: groups × genotypes; groups: PD and HC, genotypes: GG carriers and GA/AA carriers) adjusting for age, gender, and education years. Thresholds were set at a corrected P < 0.001, determined by Monte Carlo simulation. The color bar indicates the F values from ANCOVA. ALFF, amplitude of low‐frequency fluctuations; PD, Parkinson’s disease; HC, healthy control; BST1, bone marrow stromal cell antigen‐1; R, right; L, left

    Journal: CNS Neuroscience & Therapeutics

    Article Title: BST1 rs4698412 allelic variant increases the risk of gait or balance deficits in patients with Parkinson’s disease

    doi: 10.1111/cns.13099

    Figure Lengend Snippet: Groups × genotypes ANCOVA of ALFF. (A) Main effect of diagnostic groups on ALFF in PD and HC was shown in the left cerebellum_8 and cerebellum_9. (B) Interaction between BST1 rs4698412 alleles and diagnostic groups was found in the right lingual gyrus. These findings were obtained via two‐way factorial analysis of covariance (ANCOVA: groups × genotypes; groups: PD and HC, genotypes: GG carriers and GA/AA carriers) adjusting for age, gender, and education years. Thresholds were set at a corrected P < 0.001, determined by Monte Carlo simulation. The color bar indicates the F values from ANCOVA. ALFF, amplitude of low‐frequency fluctuations; PD, Parkinson’s disease; HC, healthy control; BST1, bone marrow stromal cell antigen‐1; R, right; L, left

    Article Snippet: The BST1 rs4698412 data were processed and analyzed using MassARRAY TYPER 4.0 software (Agena Bioscience, San Diego, CA, USA) via the Beijing Genomics Institute (BGI).

    Techniques: Diagnostic Assay, Control

    The interaction of groups (PD or HC) and genotypes (GG or GA/AA) on ALFF values. ***Post hoc tests were corrected by Bonferroni correction with a significant different P < 0.008 (0.05/6 [number of pair‐comparisons]). The ALFF values were decreased in the right lingual gyrus in PD patients with GA/AA carriers compared to GG carriers and increased in HC subjects. ALFF, amplitude of low‐frequency fluctuations; PD, Parkinson’s disease; HC, healthy control; BST1, bone marrow stromal cell antigen‐1

    Journal: CNS Neuroscience & Therapeutics

    Article Title: BST1 rs4698412 allelic variant increases the risk of gait or balance deficits in patients with Parkinson’s disease

    doi: 10.1111/cns.13099

    Figure Lengend Snippet: The interaction of groups (PD or HC) and genotypes (GG or GA/AA) on ALFF values. ***Post hoc tests were corrected by Bonferroni correction with a significant different P < 0.008 (0.05/6 [number of pair‐comparisons]). The ALFF values were decreased in the right lingual gyrus in PD patients with GA/AA carriers compared to GG carriers and increased in HC subjects. ALFF, amplitude of low‐frequency fluctuations; PD, Parkinson’s disease; HC, healthy control; BST1, bone marrow stromal cell antigen‐1

    Article Snippet: The BST1 rs4698412 data were processed and analyzed using MassARRAY TYPER 4.0 software (Agena Bioscience, San Diego, CA, USA) via the Beijing Genomics Institute (BGI).

    Techniques: Control