1× lds (lithium dodecyl sulfate) sample loading buffer (Thermo Fisher)

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Thermo Fisher 1× lds (lithium dodecyl sulfate) sample loading buffer
1× Lds (Lithium Dodecyl Sulfate) Sample Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lithium+loading+buffer/pm39482359-326-10-17
Average 90 stars, based on 1 article reviews

1× lds (lithium dodecyl sulfate) sample loading buffer - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Hypoxia-induced histone methylation and NF-κB activation in pancreas cancer fibroblasts promote EMT-supportive growth factor secretion
Article Snippet: 20 μg of protein were mixed with 10× NuPAGE reducing agent (Invitrogen: #NP0005), 4× LDS sample buffer (Invitrogen: NP0007), and ultrapure water.

Article Title: Cell Membrane-Integrated Neuroligin-1 Regulates the Anti-Inflammatory Effects of CRC Cell-Derived Exosomes.
Article Snippet: The beads were pelleted and washed 3 times with lysis buffer at 1000× g for 5 min. After the fourth wash, the supernatant was aspirated and the beads were resuspended in 50 μL of LDS sample buffer (1×, NuPAGETM, Invitrogen, Waltham, MA, USA) and boiled at 95 ◦C for 8 min. At the end, the sample buffer was centrifuged at 10,000× g for 1 min at 4 ◦C and the protein solution was analyzed by SDS-PAGE electrophoresis gel, using nitrocellulose (NC) membrane.

Article Title: DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 16% paraformaldehyde Alfa Aesar Cat#43368 16% formaldehyde Thermo Fisher Cat#28906 RiboLock RNase Inhibitor Thermo Fishier Cat#EO0381 DDX3X protein MyBioSource Cat#MBS1093676 DDX18 protein Origene Cat#TP760566 DDX39B protein MyBioSource Cat#MBS203605 Myc-UPF1 protein Origene Cat#TP308018 QuantSeq 30 mRNA-Seq Library Prep Kit FWD for Illumina Lexogen Cat#015.96 QuantSeq 30 mRNA-Seq V2 Library Prep Kit FWD with UDI Lexogen Cat#191.96 UMI Second Strand Synthesis Module for QuantSeq FWD Lexogen Cat#018.96 Dynabeads MyOne streptavidin C1 magnetic beads Invitrogen Cat#65002 BS3 (bis(sulfosuccinimidyl)suberate) ThermoFisher Cat#21580 LDS sample loading buffer Invitrogen Cat#NP0008 Trypsin/Lys-C Promega Cat#V5071 Iodoacetamide Sigma Cat#I1149-5G Dithiothreitol MP Biomedicals Cat#856126 OMIX C18 pipet tips Agilent Technologies Cat#A57003100 IgG CST Cat#2729 Protein G dynabeads Invitrogen Cat#10004D Ambion RNAse I ThermoFisher Cat#AM2294 AMPure XP beads Beckman Cat#A63880 4% agarose E-Gel with SYBR Safe DNA Gel Stain ThermoFisher Cat#A42136 anti-HA magnetic beads ThermoFisher Cat#88837 RNaseA Qiagen Cat#1032724 Duolink In Situ Mounting Medium with DAPI Sigma Cat#DUO82040-5ML MEGAscript T7 transcription kit ThermoFisher Cat#AM1334 Bio-11-UTP ThermoFisher Cat#AM8450 Dimethyl Sulfate Sigma Cat#D186309 Critical commercial assays LSM880 laser scanning confocal microscope Zeiss Cat#LSM880 DynabeadsTM mRNA DIRECTTM Purification Kit Invitrogen Cat#61012 Duolink In Situ Orange Starter Kit Mouse/Rabbit Sigma Cat#DUO92102-1KT Qiagen gel extraction kit Qiagen Cat#28706X4 RNeasy Plus Kit QIAGEN Cat#74136 iScript cDNA Synthesis Kit Bio-Rad Cat#1708891 PCR purification kit Qiagen Cat#28106 SMARTer Stranded Total RNA-SeqKit v3 -Pico Input Mammalian Takara Cat#634485 Human Keratinocyte NucleofectorTM Kit Lonza Cat#VPD-1002 NEB Ultra II (non-directional) kit NEB Cat#E6111 ADP-Glo kinase assay kit Promega Cat#V6930 Deposited data RNA-seq in keratinocytes dbGAP phs003766.v1.p1 RNA-seq in cervical cell and mouse cell Gene Expression Omnibus GSE252442 CLIP-seq dbGAP phs003766.v1.p1 (Continued on next page) Cell Reports 44, 115174, January 28, 2025 19

Article Title: Proinflammatory cytokines mediate pancreatic β-cell specific alterations to Golgi morphology via iNOS-dependent mitochondrial inhibition
Article Snippet: Clarified cell lysates were resuspended in LDS sample buffer (Thermo Life Technologies), resolved on 4-12 % NuPAGE gels (Thermo Life Technologies), and transferred to supported nitrocellulose membranes (BioRad).

Recombinant:

Article Title: DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs.
Article Snippet: .. For DDX50 BS3 crosslinking samples, DDX50 recombinant protein (50 ng/mL) was incubated with or without 1 mM ATP (Promega, V915A) or 1 mM glucose at room temperature for 15 min and was crosslinked following published protocol74 by 10 mM BS3 (ThermoFisher, #21580) at room temperature for 1 h and then quenched by adding LDS sample loading buffer (Invitrogen, NP0008) with 10% b-ME. .. For DDX50-STAU1 crosslinking-MS, DDX50 recombinant protein (50 ng/mL) was incubated with STAU1 recombinant protein (40 ng/mL) at room temperature for 15 min and was crosslinked following published protocol74 by 50 mM BS3 (ThermoFisher, #21580) at room temperature for 1 h and then quenched by adding LDS sample loading buffer (Invitrogen, NP0008) with 10% b-ME.

Article Title: DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs.
Article Snippet: For DDX50 BS3 crosslinking samples, DDX50 recombinant protein (50 ng/mL) was incubated with or without 1 mM ATP (Promega, V915A) or 1 mM glucose at room temperature for 15 min and was crosslinked following published protocol74 by 10 mM BS3 (ThermoFisher, #21580) at room temperature for 1 h and then quenched by adding LDS sample loading buffer (Invitrogen, NP0008) with 10% b-ME. .. For DDX50-STAU1 crosslinking-MS, DDX50 recombinant protein (50 ng/mL) was incubated with STAU1 recombinant protein (40 ng/mL) at room temperature for 15 min and was crosslinked following published protocol74 by 50 mM BS3 (ThermoFisher, #21580) at room temperature for 1 h and then quenched by adding LDS sample loading buffer (Invitrogen, NP0008) with 10% b-ME. ..

Incubation:

SDS Page:

Article Title: TIFA renders intestinal epithelial cells responsive to microbial ADP-heptose and drives colonic inflammation in mice.
Article Snippet: .. After boiling the samples in LDS sample buffer 4x (Invitrogen) for 5 minutes at 95°C, the proteins were separated by SDS-PAGE using MiniPROTEAN Precast Gels 4-15 % polyacrylamide (Bio-Rad). .. Proteins were blotted onto PVDF membranes (Bio-Rad) using the TransBlot Turbo Transfer System (Bio-Rad).

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