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lambda zap express cdna synthesis kit  (Agilent technologies)


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    Agilent technologies lambda zap express cdna synthesis kit
    Lambda Zap Express Cdna Synthesis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+zap+express+kit/pm17933557-63-1-7
    Average 90 stars, based on 1 article reviews
    lambda zap express cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Molecular and functional characterization of hemocyanin of the giant African millipede, Archispirostreptus gigas.
    Article Snippet: Poly(A)+ RNA was purified from total RNA using a PolyATract kit (Promega, Madison, WI, USA); 5μg poly(A)+ RNA was then used for the construction of a directionally cloned cDNA expression library with the Lambda ZAP-cDNA synthesis kit (Stratagene, Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Globins and hypoxia adaptation in the goldfish, Carassius auratus.
    Article Snippet: Poly(A)+RNA was purified from total RNA using the PolyATractTM kit (Promega); 5 lg poly(A)+RNA were used for the construction of a FEBS Journal 275 (2008) 3633–3643 a 2008 The Authors Journal compilation a 2008 FEBS 3639 directionally cloned cDNA expression library applying the Lambda ZAP-cDNA synthesis kit (Stratagene, Heidelberg, Germany) according to the manufacturer’s instruction.

    Article Title: Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory-secretory products of Clonorchis sinensis adult worms.
    Article Snippet: 1 Division of Malaria and Parasitic Diseases, National Institute of Health, Seoul, Korea 2 Lab of Pathogenic Proteomics, National Institute of Health, Seoul, Korea 3 Department of Parasitology and Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju, Korea 4 BK21 Ubiquitous Information Appliances, Chonnam National University, Gwangju, Korea 5 Department of Biology (the Second Stage BK21 Program), College of Natural Sciences, Chonnam National University, Gwangju, Korea 6 Division of Translational Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea 7 Department of Parasitology, Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon, Korea

    Article Title: Limulus polyphemus hemocyanin: 10 A cryo-EM structure, sequence analysis, molecular modelling and rigid-body fitting reveal the interfaces between the eight hexamers.
    Article Snippet: 0022-2836/$ see front matter © 2006 E The blue copper protein hemocyanin from the horseshoe crab Limulus polyphemus is among the largest respiratory proteins found in nature (3.5 MDa) and exhibits a highly cooperative oxygen binding.. Its 48 subunits are arranged as eight hexamers (1×6mers) that form the native 8×6mer in a nested hierarchy of 2×6mers and 4×6mers.. This quaternary structure is established by eight subunit types (termed I, IIA, II, IIIA, IIIB, IV, V, and VI), of which only type II has been sequenced.

    cDNA Library Assay:

    Article Title: Transcriptional profiling of the chick pineal gland, a photoreceptive circadian oscillator and pacemaker.
    Article Snippet: On d 8 at midday (ZT-6) and midnight (ZT-18), chicks were killed, decapitated, and their pineal glands removed and pooled. mRNA was extracted using a MicroPolyA Pure Kit (Ambion). .. The mRNA was subsequently used to generate two cDNA libraries using a Lambda Zap cDNA Library Synthesis Kit (Stratagene, La Jolla, CA). ..

    Plasmid Preparation:

    Article Title: Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation.
    Article Snippet: Poly(A)+ RNA was purified using Dynabeads Oligo (dT)25 (Dynal). .. The cDNA was synthesised from the poly(A)+ RNA and then ligated to lambda phage vector using the lambda ZAP Express cDNA Synthesis Kit (Stratagene) according to the manufacturer’s instructions. .. In vitro packaging was carried out using Gigapack Gold III Packaging Extract (Stratagene).

    Article Title: Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis.
    Article Snippet: Contents lists available at ScienceDirect Comparative Biochemistry and Physiology, Part B j ourna l homepage: www.e lsev ie r.com/ locate /cbpb Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): Gene structure, genomic organization, and mRNA expression analysis Young Sun Cho, Sang Yoon Lee, Keun-Yong Kim, Yoon Kwon Nam ⁎ Department of Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea ⁎ Corresponding author.. Tel.. : +82 51 629 5918; fax: + E-mail address: yoonknam@pknu.ac.kr (Y.K.

    cDNA Synthesis:

    Article Title: Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation.
    Article Snippet: Poly(A)+ RNA was purified using Dynabeads Oligo (dT)25 (Dynal). .. The cDNA was synthesised from the poly(A)+ RNA and then ligated to lambda phage vector using the lambda ZAP Express cDNA Synthesis Kit (Stratagene) according to the manufacturer’s instructions. .. In vitro packaging was carried out using Gigapack Gold III Packaging Extract (Stratagene).

    Article Title: Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis.
    Article Snippet: Contents lists available at ScienceDirect Comparative Biochemistry and Physiology, Part B j ourna l homepage: www.e lsev ie r.com/ locate /cbpb Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): Gene structure, genomic organization, and mRNA expression analysis Young Sun Cho, Sang Yoon Lee, Keun-Yong Kim, Yoon Kwon Nam ⁎ Department of Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea ⁎ Corresponding author.. Tel.. : +82 51 629 5918; fax: + E-mail address: yoonknam@pknu.ac.kr (Y.K.

    Article Title: EST sequencing of Onychophora and phylogenomic analysis of Metazoa.
    Article Snippet: Onychophora (velvet worms) represent a small animal taxon considered to be related to Euarthropoda.. We have obtained 1873 50 cDNA sequences (expressed sequence tags, ESTs) from the velvet worm Epiperipatus sp., which were assembled into 833 contigs.. BLAST similarity searches revealed that 51.9% of the contigs had matches in the protein databases with expectation values lower than 10 .

    Ligation:

    Article Title: Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis.
    Article Snippet: Contents lists available at ScienceDirect Comparative Biochemistry and Physiology, Part B j ourna l homepage: www.e lsev ie r.com/ locate /cbpb Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): Gene structure, genomic organization, and mRNA expression analysis Young Sun Cho, Sang Yoon Lee, Keun-Yong Kim, Yoon Kwon Nam ⁎ Department of Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea ⁎ Corresponding author.. Tel.. : +82 51 629 5918; fax: + E-mail address: yoonknam@pknu.ac.kr (Y.K.

    In Vitro:

    Article Title: Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis.
    Article Snippet: Contents lists available at ScienceDirect Comparative Biochemistry and Physiology, Part B j ourna l homepage: www.e lsev ie r.com/ locate /cbpb Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): Gene structure, genomic organization, and mRNA expression analysis Young Sun Cho, Sang Yoon Lee, Keun-Yong Kim, Yoon Kwon Nam ⁎ Department of Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea ⁎ Corresponding author.. Tel.. : +82 51 629 5918; fax: + E-mail address: yoonknam@pknu.ac.kr (Y.K.

    Cloning:

    Article Title: EST sequencing of Onychophora and phylogenomic analysis of Metazoa.
    Article Snippet: Onychophora (velvet worms) represent a small animal taxon considered to be related to Euarthropoda.. We have obtained 1873 50 cDNA sequences (expressed sequence tags, ESTs) from the velvet worm Epiperipatus sp., which were assembled into 833 contigs.. BLAST similarity searches revealed that 51.9% of the contigs had matches in the protein databases with expectation values lower than 10 .



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