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lambda gt11 vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda gt11 vector
    Lambda Gt11 Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+vector+system+gt11/us08227584-367-12-15
    Average 90 stars, based on 1 article reviews
    lambda gt11 vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Selective oxidation of 5-methylcytosine by TET-family proteins
    Article Snippet: .. Suitable vectors include, but are not limited to, the following viral vectors such as lambda vector system gt11, gt WES.tB, Charon 4, and plasmid vectors such as pBR322, pBR325, pACYC177, pACYC184, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pKC37, pKC101, SV 40, pBluescript II SK+/− or KS+/− (see “Stratagene Cloning Systems” Catalog (1993) from Stratagene, La Jolla, CA, which is hereby incorporated by reference in its entirety), pQE, pIH821, pGEX, pET series (see F. W. Studier et. al., “Use of T7 RNA Polymerase to Direct Expression of Cloned Genes,” Gene Expression Technology Vol. ..

    Article Title: Endoglucanases
    Article Snippet: .. An AEPII1a genomic library was constructed in the Lambda gt11 cloning vector (Stratagene Cloning Systems). .. The library was screened in Y1090 E. coli cells (Stratagene) for endoglucanase activity and a positive clone was identified and isolated.

    Article Title: Ostertagia vaccine
    Article Snippet: Three μg of mRNA was converted into first strand cDNA with random hexamer primers (SuperScript® Choice System for cDNA Synthesis, GibcoBRL, Life Technologies). .. Double stranded cDNA was modified with EcoRI-NotI adapters and cloned into the lambda gt11 vector (Stratagene). .. Recombinant lambda phages were packaged (Gigapack®III Gold Packaging Extract, Stratagene) and the packaging reaction was titrated.

    Cloning:

    Article Title: Selective oxidation of 5-methylcytosine by TET-family proteins
    Article Snippet: .. Suitable vectors include, but are not limited to, the following viral vectors such as lambda vector system gt11, gt WES.tB, Charon 4, and plasmid vectors such as pBR322, pBR325, pACYC177, pACYC184, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pKC37, pKC101, SV 40, pBluescript II SK+/− or KS+/− (see “Stratagene Cloning Systems” Catalog (1993) from Stratagene, La Jolla, CA, which is hereby incorporated by reference in its entirety), pQE, pIH821, pGEX, pET series (see F. W. Studier et. al., “Use of T7 RNA Polymerase to Direct Expression of Cloned Genes,” Gene Expression Technology Vol. ..

    Article Title: Endoglucanases
    Article Snippet: .. An AEPII1a genomic library was constructed in the Lambda gt11 cloning vector (Stratagene Cloning Systems). .. The library was screened in Y1090 E. coli cells (Stratagene) for endoglucanase activity and a positive clone was identified and isolated.

    Expressing:

    Article Title: Selective oxidation of 5-methylcytosine by TET-family proteins
    Article Snippet: .. Suitable vectors include, but are not limited to, the following viral vectors such as lambda vector system gt11, gt WES.tB, Charon 4, and plasmid vectors such as pBR322, pBR325, pACYC177, pACYC184, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pKC37, pKC101, SV 40, pBluescript II SK+/− or KS+/− (see “Stratagene Cloning Systems” Catalog (1993) from Stratagene, La Jolla, CA, which is hereby incorporated by reference in its entirety), pQE, pIH821, pGEX, pET series (see F. W. Studier et. al., “Use of T7 RNA Polymerase to Direct Expression of Cloned Genes,” Gene Expression Technology Vol. ..

    Article Title: Preparation of protective antigen
    Article Snippet: Restriction enzyme sites at the 3′-ends of the leader sequence-rPA sequence “cassettes” are variable and engineered to be compatible with choice of E. coli expression vector. .. The choice of available E. coli expression vectors is varied and examples include: pMTL series of vectors (proprietary to HPA), pET series (Novagen), pRSET, pET and pBAD series (Invitrogen), pQE series (Qiagen), pMal series (New England Biolabs), pPROTet series (Clontech), pGEX series (GE Healthcare), pEK/LIC series (EMD Biosciences), pIVEX series for cell-free expression (Roche) and bacteriophage Lambda gt11 and Lambda ZAP expression vectors (Stratagene). ..

    Article Title: Impaired salivary gland activity in patients with autoimmune polyendocrine syndrome type I.
    Article Snippet: Autoimmune polyendocrine syndrome type I (APS-I) is a severe disease caused by mutations in the autoimmune regulator (AIRE) gene.. We hypothesized that salivary gland dysfunction could be a possible unexplored component of these patients and here aimed to investigate salivary and lachrymal symptoms in the Norwegian cohort of APS-I patients (N1⁄4 41) and the aetiology behind it.. Sicca symptoms and possible corresponding underlying factors were assessed by subjective reports combined with objective measures of saliva and tear flow, serological testing, immune fluorescence microscopy, ultrasonography and searching for putative autoantibodies in the salivary glands.

    Article Title: Preparation of protective antigen
    Article Snippet: .. The choice of available E.coli expression vectors is varied and examples include: pMTL series of vectors (proprietary to HPA), pET series (Novagen), pRSET, pET and pBAD series (Invitrogen), pQE series (Qiagen), pMaI series (New England Biolabs), pPROTet series (Clontech), pGEX series (GE Healthcare), pEK/LIC series (EMD Biosciences), pIVEX series for cell-free expression (Roche) and bacteriophage Lambda gt11 and Lambda ZAP expression vectors (Stratagene). ..

    Clone Assay:

    Article Title: Selective oxidation of 5-methylcytosine by TET-family proteins
    Article Snippet: .. Suitable vectors include, but are not limited to, the following viral vectors such as lambda vector system gt11, gt WES.tB, Charon 4, and plasmid vectors such as pBR322, pBR325, pACYC177, pACYC184, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pKC37, pKC101, SV 40, pBluescript II SK+/− or KS+/− (see “Stratagene Cloning Systems” Catalog (1993) from Stratagene, La Jolla, CA, which is hereby incorporated by reference in its entirety), pQE, pIH821, pGEX, pET series (see F. W. Studier et. al., “Use of T7 RNA Polymerase to Direct Expression of Cloned Genes,” Gene Expression Technology Vol. ..

    Article Title: Ostertagia vaccine
    Article Snippet: Three μg of mRNA was converted into first strand cDNA with random hexamer primers (SuperScript® Choice System for cDNA Synthesis, GibcoBRL, Life Technologies). .. Double stranded cDNA was modified with EcoRI-NotI adapters and cloned into the lambda gt11 vector (Stratagene). .. Recombinant lambda phages were packaged (Gigapack®III Gold Packaging Extract, Stratagene) and the packaging reaction was titrated.

    Gene Expression:

    Article Title: Selective oxidation of 5-methylcytosine by TET-family proteins
    Article Snippet: .. Suitable vectors include, but are not limited to, the following viral vectors such as lambda vector system gt11, gt WES.tB, Charon 4, and plasmid vectors such as pBR322, pBR325, pACYC177, pACYC184, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pKC37, pKC101, SV 40, pBluescript II SK+/− or KS+/− (see “Stratagene Cloning Systems” Catalog (1993) from Stratagene, La Jolla, CA, which is hereby incorporated by reference in its entirety), pQE, pIH821, pGEX, pET series (see F. W. Studier et. al., “Use of T7 RNA Polymerase to Direct Expression of Cloned Genes,” Gene Expression Technology Vol. ..

    other:

    Article Title: Cloning, expression, and mapping of allergenic determinants of alphaS1-casein, a major cow's milk allergen.
    Article Snippet: The double-stranded cDNA was methylated, ligated to EcoRI linkers, digested with EcoRI, and inserted into dephosphorylated gt11 EcoRI-cut arms and packed into phages using the Lambda gt11/ EcoR I/CIAP-Treated/Gigapack III cloning kit (Stratagene).

    cDNA Library Assay:

    Article Title: Impaired salivary gland activity in patients with autoimmune polyendocrine syndrome type I.
    Article Snippet: Autoimmune polyendocrine syndrome type I (APS-I) is a severe disease caused by mutations in the autoimmune regulator (AIRE) gene.. We hypothesized that salivary gland dysfunction could be a possible unexplored component of these patients and here aimed to investigate salivary and lachrymal symptoms in the Norwegian cohort of APS-I patients (N1⁄4 41) and the aetiology behind it.. Sicca symptoms and possible corresponding underlying factors were assessed by subjective reports combined with objective measures of saliva and tear flow, serological testing, immune fluorescence microscopy, ultrasonography and searching for putative autoantibodies in the salivary glands.

    Immunopeptidomics:

    Article Title: Impaired salivary gland activity in patients with autoimmune polyendocrine syndrome type I.
    Article Snippet: Autoimmune polyendocrine syndrome type I (APS-I) is a severe disease caused by mutations in the autoimmune regulator (AIRE) gene.. We hypothesized that salivary gland dysfunction could be a possible unexplored component of these patients and here aimed to investigate salivary and lachrymal symptoms in the Norwegian cohort of APS-I patients (N1⁄4 41) and the aetiology behind it.. Sicca symptoms and possible corresponding underlying factors were assessed by subjective reports combined with objective measures of saliva and tear flow, serological testing, immune fluorescence microscopy, ultrasonography and searching for putative autoantibodies in the salivary glands.

    Extraction:

    Article Title: Improved quantitative PCR protocols for adenovirus and CMV with an internal inhibition control system and automated nucleic acid isolation.
    Article Snippet: With the establishment of routine virus load (DNAemia) screening for Human adenovirus (HAdV) and Cytomegalovirus (CMV) in posttransplant care quality standards for quantitative PCR-assays are increasing.. Established real-time PCR assays were improved with a fully automated DNA-extraction and with a competitive internal control DNA packaged into a lambda phage which serves as an extraction and amplification control in each sample.. HAdV and CMV DNA were detected and quantified simultaneously in various types of diagnostic samples like blood, feces or respiratory tract materials.

    Construct:

    Article Title: Endoglucanases
    Article Snippet: .. An AEPII1a genomic library was constructed in the Lambda gt11 cloning vector (Stratagene Cloning Systems). .. The library was screened in Y1090 E. coli cells (Stratagene) for endoglucanase activity and a positive clone was identified and isolated.

    Modification:

    Article Title: Ostertagia vaccine
    Article Snippet: Three μg of mRNA was converted into first strand cDNA with random hexamer primers (SuperScript® Choice System for cDNA Synthesis, GibcoBRL, Life Technologies). .. Double stranded cDNA was modified with EcoRI-NotI adapters and cloned into the lambda gt11 vector (Stratagene). .. Recombinant lambda phages were packaged (Gigapack®III Gold Packaging Extract, Stratagene) and the packaging reaction was titrated.



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