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lambda embl4 arms (Agilent technologies)

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Agilent technologies lambda embl4 arms
Lambda Embl4 Arms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+embl4/us06908615-189-20-25
Average 90 stars, based on 1 article reviews

lambda embl4 arms - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Lim1 related homeobox gene (HpLim1) expressed in sea urchin embryos.
Article Snippet: Isolated cDNA was inserted into an EcoR1 site of lgt10 vector by lgt10/EcoR1/CIAPtreated vector kit (Stratagene, La Jolla, CA, USA) and packaged using Gigapack III Gold Packaging Extract (Stratagene).

Article Title: Rehydratable food
Article Snippet: The cDNA was resuspended in 9 μL EcoR1 (an endonuclease enzyme isolated from strains of E. coli) adapters (Stratagene) and incubated at 8 degrees centigrade overnight with 4 units of T4 DNA ligase (Stratagene) (T4 is a bacteriophage of E. coli) in 1× ligase buffer (Stratagene)+1 mM ribonucleotide ATP (rATP) (Stratagene).

Article Title: Transcriptional profiling of the spleen in progressive visceral leishmaniasis reveals mixed expression of type 1 and type 2 cytokine-responsive genes
Article Snippet: The dscDNAs were then directionally cloned into EcoR1-NotI digested pBluescript II SK + phagemid vector (Stratagene).

Article Title: Nucleic acid encoding pigment protein from coral tissue
Article Snippet: 162, 156–159 (1987)). (ii) cDNA made with SMART PCR cDNA Synthesis Kit from CLONTECH (iii) cDNA ligated into Stratagene LambdaZapII EcoR1 cut/CIAP and packaged by Stratagene Gigapack II.

Article Title: The Songbird Neurogenomics (SoNG) Initiative: Community-based tools and strategies for study of brain gene function and evolution
Article Snippet: The cDNA was then directionally cloned into EcoR1-NotI digested pBluescript II SK(+) vector (Stratagene).

Ligation:

Article Title: The construction of a cDNA library enriched for immune genes and the analysis of 7535 ESTs from Chinese mitten crab Eriocheir sinensis.
Article Snippet: Chinese mitten crab Eriocheir sinensis is one of the most important aquaculture crustacean species in China.. A cDNA library was constructed from hemocytes of E. sinensis challenged with the mixture of Listonella anguillarum and Staphylococcus aureus, and randomly sequenced to collect genomic information and identify genes involved in immune defense response.. Single-pass 50 sequencing of 10368 clones yielded 7535 high quality ESTs (Expressed Sequence Tags) and these ESTs were assembled into 2943 unigenes.

Cloning:

Article Title: Methods and compositions for NAD(P)(H) oxidases
Article Snippet: .. Cloning Nox-specific DNA from L. sanfranciscensis was ligated into pBluescript (Stratagene, La Jolla) using EcoR1 (5′) and HindIII (3′) restriction sites and accordingly, nox from B. burgdorferi with BamH1(5′) and HindIII (3′) restriction sites. ..